KEGG   ENZYME: 1.14.13.113
Entry
EC 1.14.13.113              Enzyme                                 

Name
FAD-dependent urate hydroxylase;
HpxO enzyme;
FAD-dependent urate oxidase;
urate hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
urate,NADH:oxygen oxidoreductase (5-hydroxyisourate forming)
Reaction(IUBMB)
urate + NADH + H+ + O2 = 5-hydroxyisourate + NAD+ + H2O [RN:R09514]
Reaction(KEGG)
R09514
Substrate
urate [CPD:C00366];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
5-hydroxyisourate [CPD:C11821];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
A flavoprotein. The reaction is part of the purine catabolic pathway in the bacterium Klebsiella pneumoniae. The enzyme is different from EC 1.7.3.3, factor-independent urate hydroxylase, found in most plants, which produces hydrogen peroxide. The product of the enzyme is a substrate for EC 3.5.2.17, hydroxyisourate hydrolase.
History
EC 1.14.13.113 created 2010
Pathway
ec00230  Purine metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K16839  FAD-dependent urate hydroxylase
K22879  FAD-dependent urate hydroxylase
Genes
CRE: CHLREDRAFT_104037
VCN: VOLCADRAFT_86764
CVR: CHLNCDRAFT_21314
ENC: ECL_02668
ENL: A3UG_07945
ECLG: EC036_15290
ECLN: ECNIH4_14645
EAU: DI57_10870
EKB: BFV64_07975
ENO: ECENHK_07995
EEC: EcWSU1_01566(nagX)
ECHG: FY206_09190(hpxO)
ESH: C1N69_08055(hpxO)
EBG: FAI37_21585(hpxO)
KPN: KPN_01663
KPU: KP1_2709
KPP: A79E_2563
KPR: KPR_2563
KPJ: N559_2645
KPX: PMK1_03994(hpxO)
KPNU: LI86_13180
KPNK: BN49_2770
KVA: Kvar_2665
KPE: KPK_2712
KOX: KOX_16735
KOE: A225_2194
EAE: EAE_19215
EAR: CCG30039
KLW: DA718_17870(hpxO)
RAO: DSD31_16360(hpxO)
RTG: NCTC13098_04816(nagX)
REE: electrica_03279(hpxO)
LNI: CWR52_04300(hpxO)
BUF: D8682_01995(hpxO)
SGOE: A8O29_012155(hpxO)
SPE: Spro_2318
SRL: SOD_c21490(hpxO)
SPLY: Q5A_011910(hpxO)
SMAF: D781_1702
SQU: E4343_04600(hpxO)
SFJ: SAMEA4384070_2390(xlnD)
RAA: Q7S_20625
DDQ: DDI_0294
BRB: EH207_15855(hpxO)
BNG: EH206_22785(hpxO)
IZH: FEM41_11980(hpxO)
EAM: EAMY_1750
EAY: EAM_1718
ETA: ETA_17480
EPY: EpC_18390
EGE: EM595_1762(hpxO)
PAM: PANA_1960(aba2)
PAJ: PAJ_1292(aba2)
PSTW: DSJ_14010
TPTY: NCTC11468_01923(nagX) NCTC11468_02749(xlnD)
XBO: XBJ1_0311
XBV: XBW1_4476
PSI: S70_02750
PSX: DR96_3720
XCC: XCC0279
XCB: XC_0289
XCP: XCR_4231
XCV: XCV0304
XAX: XACM_0281
XAC: XAC0296
XCI: XCAW_00696(trkA)
XOO: XOO4365
XOR: XOC_4624
XPH: XppCFBP6546_10400(XppCFBP6546P_10400)
PSD: DSC_09815
PSB: Psyr_2537
PSYR: N018_14590
PLUL: FOB45_03415(hpxO)
PSES: PSCI_0902
ACB: A1S_3356
ABM: ABSDF3498
ABY: ABAYE0129
ABN: AB57_3806
ABB: ABBFA_00123(hpxO_1)
ABX: ABK1_3605
ABZ: ABZJ_03745(hpxO)
ABH: M3Q_95
ABAD: ABD1_32520
ABAZ: P795_0640
ABAU: IX87_13830
ABAA: IX88_01560
ACC: BDGL_002824(aba2)
ACI: ACIAD3540
ASJ: AsACE_CH00104(hpxO)
AID: CTZ23_00505(hpxO)
ACUM: C9E88_012055(hpxO)
AGU: AS4_22300
ALW: FOB21_03635(hpxO)
ADS: FPL17_02805(hpxO)
ATN: FM020_03480(hpxO)
PPHE: PP2015_626
PSPO: PSPO_b1793
MVS: MVIS_1436
LOK: Loa_01595
CSA: Csal_1784
MARD: IBG28_12455(hpxO)
AMAH: DLM_1384
RSO: RSc1881
RSE: F504_1880
RPI: Rpic_2082
REH: H16_A1586(trkA1)
CGD: CR3_1449(trkA)
BTD: BTI_3700
BVE: AK36_4649
BCN: Bcen_3466
BCEN: DM39_4539
BMU: Bmul_5104
BMK: DM80_4281
BMUL: NP80_3619
BSEM: WJ12_23030
BPSL: WS57_10830
BMEC: WJ16_22065
BUK: MYA_3736
BUL: BW21_5699
PPNO: DA70_22775
PPNM: LV28_07945
PPUL: RO07_02085
PSPU: NA29_09505
PAPI: SG18_02365
CABA: SBC2_37640(hpyO)
BAV: BAV1564
BHO: D560_3852
BHM: D558_3825
AMIM: MIM_c03090
ODI: ODI_R2747
AAV: Aave_4505
AJS: Ajs_3884
VEI: Veis_0812
CTES: O987_01935
MHUA: MCHK_2829
SME: SM_b20280
SMER: DU99_31965
SMD: Smed_3851
AVI: Avi_5281
RLE: RL1041
RLG: Rleg_0669
BRA: BRADO1796
BRS: S23_46980
AOL: S58_57470
BRAD: BF49_1409
XAU: Xaut_2767
MET: M446_1514
MAQU: Maq22A_1p31745(trkA) Maq22A_c06055(trkA)
BID: Bind_1848
HDN: Hden_1335
HDI: HDIA_0374
RDE: RD1_0284
CID: P73_3149
MALU: KU6B_15890
SMAZ: LH19_05595
SPHI: TS85_03625
SSAN: NX02_01460
GOX: GOX0905
GDI: GDI1999
GDJ: Gdia_0222
BMQ: BMQ_1875
BMD: BMD_1868
BMEG: BG04_4208
BACW: QR42_04755
BFD: NCTC4823_00228(xlnD)
PASA: BAOM_5064
PSAB: PSAB_09145
PRI: PRIO_2419
MPA: MAP_4203
MAO: MAP4_4328
MAVI: RC58_21500
MAVU: RE97_21550
MAV: MAV_4429
MIT: OCO_43190
MIA: OCU_42960
MID: MIP_06487
MYO: OEM_43430
MIR: OCQ_44310
MUL: MUL_0460
MMC: Mmcs_5348
MKM: Mkms_5437
MJL: Mjls_5727
MMI: MMAR_4944
MMM: W7S_21580
MHAD: B586_12100
MVQ: MYVA_0046
MCHE: BB28_05800
MSTE: MSTE_01130(aba2)
MSAL: DSM43276_01036(hpxO_1)
MTER: 4434518_03282(xlnD)
MMIN: MMIN_10070
CVA: CVAR_2631
CTER: A606_11085
GBR: Gbro_3294
GOR: KTR9_3334
GOM: D7316_05395(hpxO_2)
GOD: GKZ92_16030(hpxO)
TPR: Tpau_2962
SRT: Srot_0187
SBH: SBI_06117
SHY: SHJG_8508
SVE: SVEN_4633
STRM: M444_15050
SPRI: SPRI_2883
SMAL: SMALA_4379
SALJ: SMD11_3780
SRJ: SRO_3129
MWA: E4K62_00465(hpxO)
MSED: E3O41_10165(hpxO)
HUM: DVJ78_05935(hpxO)
LMOI: VV02_02940
IDO: I598_1238(hpxO_2)
NCA: Noca_2658
NAL: B005_5470
TCU: Tcur_2786
SRO: Sros_5335
SACC: EYD13_07785(hpxO1)
AOI: AORI_7915
AMI: Amir_4971
SESP: BN6_60120
KAL: KALB_2368
ALO: CRK60358
SAQ: Sare_0982
MIL: ML5_1410
ASE: ACPL_1155(kmo)
AFS: AFR_06280
ACTS: ACWT_1036
CAI: Caci_6402
SNA: Snas_4581
AHW: NCTC11636_02117(nahG)
TBI: Tbis_3284
SYG: sync_1608
LET: O77CONTIG1_04142(hpxO)
THEU: HPC62_18695(hpxO)
NSH: GXM_07437
AVA: Ava_0103
CTHE: Chro_0933
RUL: UC8_47890(hpxO)
GMR: GmarT_00540(hpxO_1)
FTJ: FTUN_5324
ABAS: ACPOL_3535
NJA: NSJP_2538
HWA: HQ_1741A
HWC: Hqrw_1865
 » show all
Reference
1  [PMID:19260710]
  Authors
O'Leary SE, Hicks KA, Ealick SE, Begley TP
  Title
Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase.
  Journal
Biochemistry 48:3033-5 (2009)
DOI:10.1021/bi900160b
  Sequence
[kpn:KPN_01663]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.113
IUBMB Enzyme Nomenclature: 1.14.13.113
ExPASy - ENZYME nomenclature database: 1.14.13.113
BRENDA, the Enzyme Database: 1.14.13.113

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