Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

urate,NADH:oxygen oxidoreductase (5-hydroxyisourate forming)

urate + NADH + H+ + O2 = 5-hydroxyisourate + NAD+ + H2O [RN:R09514]

R09514

urate [CPD:C00366];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

A flavoprotein. The reaction is part of the purine catabolic pathway in the bacterium Klebsiella pneumoniae. The enzyme is different from EC 1.7.3.3, factor-independent urate hydroxylase, found in most plants, which produces hydrogen peroxide. The product of the enzyme is a substrate for EC 3.5.2.17, hydroxyisourate hydrolase.

EC 1.14.13.113 created 2010

1  [PMID:19260710]

O'Leary SE, Hicks KA, Ealick SE, Begley TP

Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase.

Biochemistry 48:3033-5 (2009)
DOI:10.1021/bi900160b

[kpn:KPN_01663]