KEGG   ENZYME: 1.14.13.113Help
Entry
EC 1.14.13.113              Enzyme                                 

Name
FAD-dependent urate hydroxylase;
HpxO enzyme;
FAD-dependent urate oxidase;
urate hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
urate,NADH:oxygen oxidoreductase (5-hydroxyisourate forming)
Reaction(IUBMB)
urate + NADH + H+ + O2 = 5-hydroxyisourate + NAD+ + H2O [RN:R09514]
Reaction(KEGG)
Substrate
urate [CPD:C00366];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
5-hydroxyisourate [CPD:C11821];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
A flavoprotein. The reaction is part of the purine catabolic pathway in the bacterium Klebsiella pneumoniae. The enzyme is different from EC 1.7.3.3, factor-independent urate hydroxylase, found in most plants, which produces hydrogen peroxide. The product of the enzyme is a substrate for EC 3.5.2.17, hydroxyisourate hydrolase.
History
EC 1.14.13.113 created 2010
Pathway
ec00230  Purine metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K16839  FAD-dependent urate hydroxylase
Genes
ENC: ECL_02668
ENO: ECENHK_07995
EEC: EcWSU1_01566(nagX)
ENL: A3UG_07945
ECLG: EC036_15290
ECLN: ECNIH4_14645
EAS: Entas_1494
EAU: DI57_10870
EKB: BFV64_07975
ELG: BH714_03925
KPN: KPN_01663
KPU: KP1_2709
KPP: A79E_2563
KPE: KPK_2712
KPR: KPR_2563
KPJ: N559_2645
KPX: PMK1_03994(hpxO)
KPNU: LI86_13180
KPNK: BN49_2770
KVA: Kvar_2665
KOX: KOX_16735
KOE: A225_2194
EAE: EAE_19215
EAR: CCG30039
SPE: Spro_2318
SRL: SOD_c21490(hpxO)
SPLY: Q5A_011910(hpxO)
SMAF: D781_1702
RAA: Q7S_20625
DDQ: DDI_0294
ETA: ETA_17480
EPY: EpC_18390
EAM: EAMY_1750
EAY: EAM_1718
EBI: EbC_22690
EGE: EM595_1762(hpxO)
PAM: PANA_1960(aba2)
PAJ: PAJ_1292(aba2)
PVA: Pvag_1367(kmo)
PSTW: DSJ_14010
ACB: A1S_3356
ABM: ABSDF3498
ABY: ABAYE0129
ABN: AB57_3806
ABX: ABK1_3605
ABH: M3Q_95
ABAD: ABD1_32520
ABAZ: P795_0640
ABAU: IX87_13830
ABAA: IX88_01560
ACC: BDGL_002824(aba2)
ACI: ACIAD3540
ASJ: AsACE_CH00104(hpxO)
SPSW: Sps_04489
CSA: Csal_1784
KVU: EIO_2118
KVL: KVU_1677
MPA: MAP_4203
MAO: MAP4_4328
MAVI: RC58_21500
MAVU: RE97_21550
MAV: MAV_4429
MAVD: NF84_20350
MAVR: LA63_20550
MAVA: LA64_20530
MIT: OCO_43190
MIA: OCU_42960
MID: MIP_06487
MYO: OEM_43430
MIR: OCQ_44310
MMI: MMAR_4944
MMM: W7S_21580
MHAD: B586_12100
MVA: Mvan_5278
MCHE: BB28_05800
MSTE: MSTE_01130(aba2)
GBR: Gbro_3294
GOR: KTR9_3334
TPR: Tpau_2962
SRT: Srot_0187
MTS: MTES_1339
IDO: I598_1238(hpxO_2)
ACTN: L083_1264(kmo)
LET: O77CONTIG1_04142(hpxO)
CTHE: Chro_0933
 » show all
Taxonomy
Reference
1  [PMID:19260710]
  Authors
O'Leary SE, Hicks KA, Ealick SE, Begley TP
  Title
Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase.
  Journal
Biochemistry 48:3033-5 (2009)
DOI:10.1021/bi900160b
  Sequence
[kpn:KPN_01663]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.113
IUBMB Enzyme Nomenclature: 1.14.13.113
ExPASy - ENZYME nomenclature database: 1.14.13.113
BRENDA, the Enzyme Database: 1.14.13.113

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