KEGG   ENZYME: 1.14.13.127Help
Entry
EC 1.14.13.127              Enzyme                                 

Name
3-(3-hydroxyphenyl)propanoate hydroxylase;
mhpA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-(3-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
(1) 3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+ [RN:R06786];
(2) (2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + H+ + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+ [RN:R06787]
Reaction(KEGG)
Substrate
3-(3-hydroxyphenyl)propanoate [CPD:C11457];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007];
(2E)-3-(3-hydroxyphenyl)prop-2-enoate [CPD:C12621]
Product
3-(2,3-dihydroxyphenyl)propanoate [CPD:C04044];
H2O [CPD:C00001];
NAD+ [CPD:C00003];
(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate [CPD:C12623]
Comment
A flavoprotein (FAD). This enzyme participates in a meta-cleavage pathway employed by the bacterium Escherichia coli for the degradation of various phenylpropanoid compounds.
History
EC 1.14.13.127 created 2011
Pathway
ec00360  Phenylalanine metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K05712  3-(3-hydroxy-phenyl)propionate hydroxylase
Genes
ECO: b0347(mhpA)
ECJ: JW0338(mhpA)
ECD: ECDH10B_1359(mhpA)
ECOK: ECMDS42_0269(mhpA)
ECE: Z0445(mhpA)
ECS: ECs0402(mhpA)
ECF: ECH74115_0422(mhpA)
ETW: ECSP_0411(mhpA)
ELX: CDCO157_0390(mhpA)
EOJ: ECO26_0383(mhpA)
EOI: ECO111_0383(mhpA)
EOH: ECO103_0329(mhpA)
EOK: G2583_0460(mhpA)
ELR: ECO55CA74_02210(mhpA)
ECX: EcHS_A0411(mhpA)
ECW: EcE24377A_0371(mhpA)
ECM: EcSMS35_0378(mhpA)
ECY: ECSE_0372
ECR: ECIAI1_0348(mhpA)
ECQ: ECED1_0375(mhpA)
ECK: EC55989_0354(mhpA)
ECT: ECIAI39_0331(mhpA)
EOC: CE10_0315(mhpA)
EUM: ECUMN_0390(mhpA)
ELO: EC042_0384(mhpA)
ELH: ETEC_0403
ESE: ECSF_0318
ESO: O3O_05555(mhpA)
ESM: O3M_19725(mhpA)
ESL: O3K_19740(mhpA)
EBR: ECB_00301(mhpA)
EBD: ECBD_3310
EKF: KO11_21845(mhpA)
EDJ: ECDH1ME8569_0334(mhpA)
ENA: ECNA114_0330(mhpA)
ELW: ECW_m0425(mhpA)
ELL: WFL_02090(mhpA)
ELP: P12B_c0307(mhpA) P12B_c0365(mhpA)
EBL: ECD_00301(mhpA)
EBE: B21_00305(mhpA)
ECOL: LY180_02125(mhpA)
ECOJ: P423_01825(mhpA)
ECOO: ECRM13514_0499(mhpA)
ECOH: ECRM13516_0327(mhpA)
ECOS: EC958_0491
EMA: C1192_10920(mhpA)
SSN: SSON_0297(mhpA)
SHQ: A0259_05405(mhpA)
KPN: KPN_02122(mhpA)
KPU: KP1_3217(mhpA)
KPP: A79E_2122
KPK: A593_25665(mhpA)
KPH: KPNIH24_12930(mhpA)
KPZ: KPNIH27_14865(mhpA)
KPV: KPNIH29_15525(mhpA)
KPW: KPNIH30_15805(mhpA)
KPY: KPNIH31_14660(mhpA)
KPG: KPNIH32_15700(mhpA)
KPC: KPNIH10_15325(mhpA)
KPQ: KPR0928_15300(mhpA)
KPT: VK055_0339(mhpA)
KPE: KPK_2201(mhpA)
KPO: KPN2242_13565(mhpA)
KPJ: N559_2167
KPI: D364_10875(mhpA)
KPX: PMK1_04486(mhpA)
KPB: FH42_06705(mhpA)
KPNE: KU54_010815(mhpA)
KPNU: LI86_10770(mhpA)
KPNK: BN49_3227(mhpA)
KVA: Kvar_2153
KVD: KR75_23585(mhpA)
KVQ: SP68_04240(mhpA)
KOX: KOX_22695(mhpA)
KOE: A225_3416
KOY: J415_14950(mhpA)
KOK: KONIH1_16730(mhpA)
KOC: AB185_19175(mhpA)
KOM: HR38_20990(mhpA)
KQU: AVR78_19690(mhpA)
KQV: B8P98_11790(mhpA)
CFD: CFNIH1_11830(mhpA)
CWE: CO701_16995(mhpA)
EBT: EBL_c20040(mhpA1)
ROR: RORB6_04275(mhpA)
RPLN: B1209_10930(mhpA)
PGE: LG71_09620(mhpA)
KGO: CEW81_11370(mhpA)
EBF: D782_1982
SERA: Ser39006_017355(mhpA)
PLU: plu1437
PAY: PAU_02254
LEZ: GLE_5300
VNA: PN96_06275(mhpA)
VEJ: VEJY3_06780(mhpA)
PCQ: PcP3B5_43040(mhpA_3)
PPUN: PP4_19460
PSYR: N018_13885
PPZ: H045_07775(mhpA)
PSTT: CH92_04330
PPUU: PputUW4_02144(mhpA)
PPSY: AOC04_16940(mhpA)
PSET: THL1_2655
PSIL: PMA3_17995
ALC: OTEC02_08885(mhpA)
AEI: AOY20_07915(mhpA)
GAI: IMCC3135_20595(mhpA)
HCO: LOKO_00974(pcpB) LOKO_00984(mhpA)
ADI: B5T_01229
APAC: S7S_05355
ZDF: AN401_03000(mhpA)
RSO: RSp0702
RSN: RSPO_m01032(ubiF)
RSE: F504_4517
RPI: Rpic_4529
REH: H16_B0971(h16_B0971) H16_B1546(h16_B1546) H16_B2557(h16_B2557)
CNC: CNE_2c15470(mhpA1) CNE_2c24670(mhpA2) CNE_BB1p14220(mhpA)
RME: Rmet_0339
CGD: CR3_3181(mhpA) CR3_4109(mhpA)
BMA: BMA2059
BMAL: DM55_2396
BMAE: DM78_2627
BMAQ: DM76_2381
BMAI: DM57_847
BMAF: DM51_1717
BMAZ: BM44_1291
BMAB: BM45_853
BPSE: BDL_2704
BPSM: BBQ_569
BPSU: BBN_697
BPSD: BBX_1102
BPK: BBK_2190
BPSH: DR55_1776
BPSA: BBU_2821
BPSO: X996_1416
BTE: BTH_I1395
BTQ: BTQ_2538
BTJ: BTJ_3156
BTZ: BTL_1087
BTD: BTI_862
BTV: BTHA_1179
BTHE: BTN_301
BTHM: BTRA_1295
BTHA: DR62_467
BTHL: BG87_1284
BCEO: I35_0670 I35_0874 I35_1234(mhpA) I35_4084(mhpA)
BDL: AK34_2355
BUB: BW23_920
BSTG: WT74_03995
BGU: KS03_3563
BPE: BP3131
BPC: BPTD_3093
BPER: BN118_2796
BPET: B1917_0691
BPEU: Q425_31090
BPA: BPP0811
BPAR: BN117_0841
BBR: BB0895
BHZ: ACR54_00736(mhpA_1) ACR54_04380(mhpA_2) ACR54_04635(mhpA_3)
BTRM: SAMEA390648701822(mhpA)
AXX: ERS451415_00865(mhpA_1) ERS451415_05371(mhpA_2) ERS451415_05492(mhpA_3)
PUT: PT7_2127
AMIM: MIM_c24060
AFQ: AFA_02775
PNA: Pnap_0616
AAA: Acav_1712
LIM: L103DPR2_02457(mhpA_1) L103DPR2_02630(mhpA_2)
LIH: L63ED372_01280(mhpA_1) L63ED372_02668(mhpA_2)
CBAA: SRAA_1361
LCH: Lcho_4300
TIN: Tint_2994
THI: THI_3545
SHD: SUTH_02820(mhpA)
EBA: ebA3297
AZO: azo3681(mhpA)
AOA: dqs_3826
CCX: COCOR_04507(mhpA)
BBA: Bd1456
MLO: mll0858
PLA: Plav_1541
SME: SMa1939
RLG: Rleg_5524
BJA: bll0346 bll2329 blr5284(mhpA)
RPA: RPA4675
RPB: RPB_0903
RPC: RPC_0888
RPD: RPD_1015
RPE: RPE_0909
RPT: Rpal_5156
NHA: Nham_0924
VGO: GJW-30_1_03692(mhpA)
AZC: AZC_2882
MNO: Mnod_1196
MAQU: Maq22A_c21910(ubiH)
MMED: Mame_00145(mhpA)
RBM: TEF_20555
CSE: Cseg_1743
SIL: SPO0682
JAN: Jann_1432
DSH: Dshi_3815
PSF: PSE_p0359
PGA: PGA1_c00410(mhpA)
PGL: PGA2_c30450(mhpA)
PHP: PhaeoP97_00271(mhpA)
PPIC: PhaeoP14_03012(mhpA)
OAR: OA238_c45820(mhpA)
MALG: MALG_01680
RSU: NHU_04299
RHC: RGUI_2337
SPSE: SULPSESMR1_02737(otcC)
LVS: LOKVESSMR4R_00436(mhpA)
AHT: ANTHELSMS3_02396(otcC)
HBA: Hbal_2586
NAR: Saro_3413
SPHP: LH20_19515
STAX: MC45_16555
SPHR: BSY17_3122
SCLO: SCLO_3000700(opdA) SCLO_4000240(nmoA)
BLAS: BSY18_2911
AZL: AZL_024700(mhpA)
BTW: BF38_4197
BACO: OXB_0850
BKW: BkAM31D_08515(pcpB)
AAD: TC41_1825
SAY: TPY_3023(mhpA)
MPA: MAP_0694
MAO: MAP4_3170
MAVI: RC58_15710
MAVU: RE97_15740
MAVD: NF84_03735
MAVR: LA63_03835
MAVA: LA64_03845
MIT: OCO_07340
MIA: OCU_07350
MID: MIP_01270
MYO: OEM_07420
MIR: OCQ_07490
MMC: Mmcs_3786
MKM: Mkms_3859
MJL: Mjls_3790
MMM: W7S_03590(mhpA)
MSM: MSMEG_2485(mhpA) MSMEG_4866(mhpA)
MGI: Mflv_2401
MPHL: MPHLCCUG_03811(mhpA)
MVQ: MYVA_4048(mhpA)
MAB: MAB_2573c
MABB: MASS_2521(mhpA2)
MABO: NF82_12855
MCHE: BB28_12825
CTER: A606_01150(mhpA)
CAMG: CAMM_02615
NFR: ERS450000_00746(mhpA_1) ERS450000_01152(mhpA_2)
RER: RER_39300
REY: O5Y_18240
RFA: A3L23_02166(mhpA)
RHS: A3Q41_01213(mhpA_1) A3Q41_02053(mhpA_2)
RHU: A3Q40_02905(mhpA_2) A3Q40_03719(mhpA_3)
TPR: Tpau_2814
SRT: Srot_1726
SCO: SCO5773(SC4H8.12c)
SMA: SAVERM_563(mhpA)
SGR: SGR_1740
SGB: WQO_26800
SFA: Sfla_1578
SVE: SVEN_5428
SALS: SLNWT_1511
STRP: F750_5287
SFI: SFUL_5696
SALU: DC74_5863
SALL: SAZ_12610
STRE: GZL_02980
SPRI: SPRI_2127
SRW: TUE45_00110(mhpA_2) TUE45_06270(mhpA_3)
SLE: sle_19000(sle_19000)
SRN: A4G23_04290(mhpA)
STRD: NI25_10355
SLAU: SLA_5642
SALF: SMD44_06397(mhpA)
SALJ: SMD11_1832(mhpA)
SLX: SLAV_11045(mhpA)
SFK: KY5_5906c
KSK: KSE_53620
CFL: Cfla_0297
KFL: Kfla_3146
NDA: Ndas_1867
NAL: B005_5046
TCU: Tcur_0725
NML: Namu_1344
AMD: AMED_5351(mhpA)
AMN: RAM_26760 RAM_27265(mhpA)
AMM: AMES_5286(mhpA)
AMZ: B737_5286(mhpA)
PDX: Psed_4218
PSEE: FRP1_28405
KAL: KALB_2278
ALL: CRK59933
MIL: ML5_4551
ASE: ACPL_5050(mhpA)
AFS: AFR_22975
ACTS: ACWT_4920
CAI: Caci_6461
CWO: Cwoe_3896
HHB: Hhub_2446
 » show all
Taxonomy
Reference
1  [PMID:6345502]
  Authors
Burlingame R, Chapman PJ.
  Title
Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli.
  Journal
J Bacteriol 155:113-21 (1983)
Reference
2  [PMID:3531186]
  Authors
Burlingame RP, Wyman L, Chapman PJ
  Title
Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation.
  Journal
J Bacteriol 168:55-64 (1986)
DOI:10.1128/JB.168.1.55-64.1986
Reference
3  [PMID:9098055]
  Authors
Ferrandez A, Garcia JL, Diaz E
  Title
Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12.
  Journal
J Bacteriol 179:2573-81 (1997)
DOI:10.1128/JB.179.8.2573-2581.1997
  Sequence
[eco:b0347]
Reference
4  [PMID:9603882]
  Authors
Diaz E, Ferrandez A, Garcia JL.
  Title
Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12.
  Journal
J Bacteriol 180:2915-23 (1998)
  Sequence
[eco:b0347]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.127
IUBMB Enzyme Nomenclature: 1.14.13.127
ExPASy - ENZYME nomenclature database: 1.14.13.127
BRENDA, the Enzyme Database: 1.14.13.127

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