KEGG   ENZYME: 1.14.13.127
Entry
EC 1.14.13.127              Enzyme                                 

Name
3-(3-hydroxyphenyl)propanoate hydroxylase;
mhpA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
3-(3-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
(1) 3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+ [RN:R06786];
(2) (2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + H+ + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+ [RN:R06787]
Reaction(KEGG)
R06786 R06787
Substrate
3-(3-hydroxyphenyl)propanoate [CPD:C11457];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007];
(2E)-3-(3-hydroxyphenyl)prop-2-enoate [CPD:C12621]
Product
3-(2,3-dihydroxyphenyl)propanoate [CPD:C04044];
H2O [CPD:C00001];
NAD+ [CPD:C00003];
(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate [CPD:C12623]
Comment
A flavoprotein (FAD). This enzyme participates in a meta-cleavage pathway employed by the bacterium Escherichia coli for the degradation of various phenylpropanoid compounds.
History
EC 1.14.13.127 created 2011
Pathway
ec00360  Phenylalanine metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K05712  3-(3-hydroxy-phenyl)propionate hydroxylase
Genes
ECO: b0347(mhpA)
ECJ: JW0338(mhpA)
ECD: ECDH10B_1359(mhpA)
ECOK: ECMDS42_0269(mhpA)
ECE: Z0445(mhpA)
ECS: ECs0402(mhpA)
ECF: ECH74115_0422(mhpA)
ETW: ECSP_0411(mhpA)
ELX: CDCO157_0390(mhpA)
EOI: ECO111_0383(mhpA)
EOJ: ECO26_0383(mhpA)
EOH: ECO103_0329(mhpA)
ECOO: ECRM13514_0499(mhpA)
ECOH: ECRM13516_0327(mhpA)
ESL: O3K_19740(mhpA)
ESO: O3O_05555(mhpA)
ESM: O3M_19725(mhpA)
ECK: EC55989_0354(mhpA)
EOK: G2583_0460(mhpA)
ELR: ECO55CA74_02210(mhpA)
ELH: ETEC_0403
ECW: EcE24377A_0371(mhpA)
ENA: ECNA114_0330(mhpA)
ECOS: EC958_0491
ECX: EcHS_A0411(mhpA)
ECM: EcSMS35_0378(mhpA)
ECY: ECSE_0372
ECR: ECIAI1_0348(mhpA)
ECQ: ECED1_0375(mhpA)
EUM: ECUMN_0390(mhpA)
ECT: ECIAI39_0331(mhpA)
EOC: CE10_0315(mhpA)
EBR: ECB_00301(mhpA)
EBL: ECD_00301(mhpA)
EBE: B21_00305(mhpA)
EBD: ECBD_3310
ELO: EC042_0384(mhpA)
ESE: ECSF_0318
EKF: KO11_21845(mhpA)
EDJ: ECDH1ME8569_0334(mhpA)
ELW: ECW_m0425(mhpA)
ELL: WFL_02090(mhpA)
ELP: P12B_c0307(mhpA) P12B_c0365(mhpA)
ECOL: LY180_02125(mhpA)
ECOJ: P423_01825(mhpA)
EMA: C1192_10920(mhpA)
SSN: SSON_0297(mhpA)
SHQ: A0259_05405(mhpA)
KPN: KPN_02122(mhpA)
KPU: KP1_3217(mhpA)
KPP: A79E_2122
KPH: KPNIH24_12930(mhpA)
KPZ: KPNIH27_14865(mhpA)
KPV: KPNIH29_15525(mhpA)
KPW: KPNIH30_15805(mhpA)
KPY: KPNIH31_14660(mhpA)
KPG: KPNIH32_15700(mhpA)
KPC: KPNIH10_15325(mhpA)
KPQ: KPR0928_15300(mhpA)
KPT: VK055_0339(mhpA)
KPO: KPN2242_13565(mhpA)
KPR: KPR_2668
KPJ: N559_2167
KPI: D364_10875(mhpA)
KPX: PMK1_04486(mhpA)
KPB: FH42_06705(mhpA)
KPNE: KU54_010815(mhpA)
KPNU: LI86_10770(mhpA)
KPNK: BN49_3227(mhpA)
KVA: Kvar_2153
KPE: KPK_2201(mhpA)
KPK: A593_25665(mhpA)
KVD: KR75_23585(mhpA)
KVQ: SP68_04240(mhpA)
KOX: KOX_22695(mhpA)
KOE: A225_3416
KOY: J415_14950(mhpA)
KOM: HR38_20990(mhpA)
KOK: KONIH1_16730(mhpA)
KOC: AB185_19175(mhpA)
KQU: AVR78_19690(mhpA)
KQV: B8P98_11790(mhpA)
KLL: BJF97_16850(mhpA)
CFD: CFNIH1_11830(mhpA)
CWE: CO701_16995(mhpA)
CYO: CD187_18595(mhpA)
CFQ: C2U38_05245(mhpA)
CPAR: CUC49_01470(mhpA)
EBT: EBL_c20040(mhpA1)
ROR: RORB6_04275(mhpA)
RPLN: B1209_10930(mhpA)
REE: electrica_02135(mhpA)
PGE: LG71_09620(mhpA)
KGO: CEW81_11370(mhpA)
EBF: D782_1982
EBU: CUC76_00380(mhpA)
SERA: Ser39006_017355(mhpA)
SERQ: CWC46_17355(mhpA)
PLU: plu1437
PAY: PAU_02254
LAB: LA76x_4323(cabe)
LAQ: GLA29479_2257(cabe)
LCP: LC55x_0853(cabe)
LGU: LG3211_1992(cabe)
LEZ: GLE_5300
LEM: LEN_2426
RBD: ALSL_1097
VNA: PN96_06275(mhpA)
VEJ: VEJY3_06780(mhpA)
PCQ: PcP3B5_26560(pnpA) PcP3B5_43040(mhpA_3)
PPUN: PP4_19460
PPUD: DW66_3565
PSYR: N018_13885
PPZ: H045_07775(mhpA)
PSTT: CH92_04330
PPUU: PputUW4_02144(mhpA)
PPSY: AOC04_16940(mhpA)
PSET: THL1_2655
PSIL: PMA3_17995
ALC: OTEC02_08885(mhpA)
AEI: AOY20_07915(mhpA)
AGU: AS4_29920
GAI: IMCC3135_20595(mhpA)
HCO: LOKO_00974(pcpB) LOKO_00984(mhpA)
ADI: B5T_01229
APAC: S7S_05355
ZDF: AN401_03000(mhpA)
RSO: RSp0702
RSN: RSPO_m01032(ubiF)
RSE: F504_4517
RPI: Rpic_4529
REH: H16_B0971(h16_B0971) H16_B1546(h16_B1546) H16_B2557(h16_B2557)
CNC: CNE_2c15470(mhpA1) CNE_2c24670(mhpA2) CNE_BB1p14220(mhpA)
RME: Rmet_0339
CGD: CR3_3181(mhpA) CR3_4109(mhpA)
BMA: BMA2059
BPK: BBK_2190
BGO: BM43_2317
CABA: SBC2_07000(mhpA_1)
BPE: BP3131
BPC: BPTD_3093
BPER: BN118_2796
BPET: B1917_0691
BPEU: Q425_31090
BPAR: BN117_0841
BPA: BPP0811
BBR: BB0895
BHO: D560_1693
BHM: D558_1682
BHZ: ACR54_00736(mhpA_1) ACR54_04380(mhpA_2) ACR54_04635(mhpA_3)
BTRM: SAMEA390648701822(mhpA)
AXX: ERS451415_00865(mhpA_1) ERS451415_05371(mhpA_2) ERS451415_05492(mhpA_3)
PUT: PT7_2127
AMIM: MIM_c24060
AFQ: AFA_02775
PNA: Pnap_0616
AAA: Acav_1712
LIM: L103DPR2_02457(mhpA_1) L103DPR2_02630(mhpA_2)
LIH: L63ED372_01280(mhpA_1) L63ED372_02668(mhpA_2)
HPSE: HPF_04845(mhpA2) HPF_10825(mhpA3) HPF_15650(mhpA4) HPF_20465(pcpB)
CBAA: SRAA_1361
LCH: Lcho_4300
TIN: Tint_2994
THI: THI_3545
SHD: SUTH_02820(mhpA)
EBA: ebA3297
OTR: OTERR_30100(mhpA)
AZO: azo3681(mhpA)
AOA: dqs_3826
SCL: sce2246(yhjG2)
HOH: Hoch_1342
MLO: mll0858
MHUA: MCHK_2533
PLA: Plav_1541
SME: SMa1939
BJA: bll0346 bll2329 blr5284(mhpA)
RPA: RPA4675
RPB: RPB_0903
RPC: RPC_0888
RPD: RPD_1015
RPE: RPE_0909
RPT: Rpal_5156
NHA: Nham_0924
VGO: GJW-30_1_03692(mhpA)
AZC: AZC_2882
MNO: Mnod_1196
MOR: MOC_3589
MAQU: Maq22A_c19785(ubiH) Maq22A_c21910(ubiH)
MMED: Mame_00145(mhpA)
RBM: TEF_20555
CSE: Cseg_1743
SIL: SPO0682
RUT: FIU92_02300(mhpA)
JAN: Jann_1432
PSF: PSE_p0359
PGA: PGA1_c00410(mhpA)
PGL: PGA2_c30450(mhpA)
PHP: PhaeoP97_00271(mhpA)
PPIC: PhaeoP14_03012(mhpA)
OAR: OA238_c45820(mhpA)
LAQU: R2C4_16655
MALG: MALG_01680
RSU: NHU_04299
RHC: RGUI_2337
LABT: FIU93_00130(mhpA1) FIU93_25800(mhpA2) FIU93_26335(mhpA3)
SPSE: SULPSESMR1_02737(otcC)
RID: RIdsm_04844(mhpA)
LVS: LOKVESSMR4R_00436(mhpA)
AHT: ANTHELSMS3_02396(otcC)
ROT: FIV09_08430(mhpA)
HBA: Hbal_2586
NAR: Saro_3413
SPHP: LH20_19515
STAX: MC45_16555
SPHI: TS85_14980
SPHR: BSY17_3122
SCLO: SCLO_3000700(opdA) SCLO_4000240(nmoA)
SFLA: SPHFLASMR4Y_02977(mhpA)
BLAS: BSY18_2911
SMIC: SmB9_08720
MGRY: MSR1_09680(mhpA)
AZL: AZL_024700(mhpA)
BBA: Bd1456
HTL: HPTL_0481
BHA: BH1998
BTW: BF38_4197
BACO: OXB_0850
BKW: BkAM31D_08515(pcpB)
AAD: TC41_1825
SAY: TPY_3023(mhpA)
MIT: OCO_07340
MIA: OCU_07350
MID: MIP_01270
MYO: OEM_07420
MIR: OCQ_07490
MMC: Mmcs_3786
MKM: Mkms_3859
MJL: Mjls_3790
MMM: W7S_03590(mhpA)
MSHG: MSG_02890 MSG_03542(mhpA_1) MSG_04315(mhpA_2)
MFJ: MFLOJ_03380(mhpA_1) MFLOJ_43000(mhpA_2)
MSM: MSMEG_2485(mhpA) MSMEG_4866(mhpA)
MGI: Mflv_2401
MPHL: MPHLCCUG_03811(mhpA)
MVQ: MYVA_4048(mhpA)
MTHN: 4412656_03105(mhpA)
MHAS: MHAS_02632(mhpA)
MDU: MDUV_04250(mhpA)
MCHT: MCHIJ_29330(mhpA_1) MCHIJ_29940(mhpA_2)
MAB: MAB_2573c
MABB: MASS_2521(mhpA2)
MCHE: BB28_12825
MSTE: MSTE_03924
MSAL: DSM43276_03570(mhpA_3)
MKR: MKOR_38630(mhpA)
CTER: A606_01150(mhpA)
CAMG: CAMM_02615
NFR: ERS450000_00746(mhpA_1) ERS450000_01152(mhpA_2)
RER: RER_39300
REY: O5Y_18240
RFA: A3L23_02166(mhpA)
RHS: A3Q41_01213(mhpA_1) A3Q41_02053(mhpA_2)
RHU: A3Q40_02905(mhpA_2) A3Q40_03719(mhpA_3)
RRT: 4535765_01429(mhpA_1) 4535765_03253(mhpA_2) 4535765_04588(mhpA_3)
TPR: Tpau_2814
SRT: Srot_1726
SCO: SCO5773(SC4H8.12c)
SMA: SAVERM_563(mhpA)
SGR: SGR_1740
SGB: WQO_26800
SFA: Sfla_1578
SVE: SVEN_5428
SALS: SLNWT_1511
STRP: F750_5287
SFI: SFUL_5696
SALU: DC74_5863
SALL: SAZ_12610
STRE: GZL_02980
SPRI: SPRI_2127
SRW: TUE45_00110(mhpA_2) TUE45_06270(mhpA_3)
SLE: sle_19000(sle_19000)
SRN: A4G23_04290(mhpA)
STRD: NI25_10355
SLAU: SLA_5642
SALF: SMD44_06397(mhpA)
SALJ: SMD11_1832(mhpA)
SLX: SLAV_11045(mhpA)
SFK: KY5_5906c
SGE: DWG14_00218(mhpA_1) DWG14_00480(mhpA_2) DWG14_02284(mhpA_3)
SRJ: SRO_2069
KSK: KSE_53620
ACH: Achl_0090
CFL: Cfla_0297
NCA: Noca_0653
KFL: Kfla_3146
NDA: Ndas_1867
NAL: B005_5046
STRR: EKD16_08965(pcpB)
TCU: Tcur_0725
NML: Namu_1344
SACC: EYD13_10210(mhpA2)
AMD: AMED_5351(mhpA)
AMN: RAM_26760 RAM_27265(mhpA)
AMM: AMES_5286(mhpA)
AMZ: B737_5286(mhpA)
AMYY: YIM_30285(pcpB4) YIM_36220(pcpB6)
PDX: Psed_4218
PSEE: FRP1_28405
ACTI: UA75_19560
ALO: CRK59933
MIL: ML5_4551
ASE: ACPL_5050(mhpA)
AFS: AFR_22975
ACTS: ACWT_4920
CAI: Caci_6461
CWO: Cwoe_3896
AFO: Afer_0814
GLJ: GKIL_2558(mhpA)
GAU: GAU_0810
GBA: J421_0988
HHB: Hhub_2446
 » show all
Reference
1  [PMID:6345502]
  Authors
Burlingame R, Chapman PJ.
  Title
Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli.
  Journal
J Bacteriol 155:113-21 (1983)
DOI:10.1128/JB.155.1.113-121.1983
Reference
2  [PMID:3531186]
  Authors
Burlingame RP, Wyman L, Chapman PJ
  Title
Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation.
  Journal
J Bacteriol 168:55-64 (1986)
DOI:10.1128/JB.168.1.55-64.1986
Reference
3  [PMID:9098055]
  Authors
Ferrandez A, Garcia JL, Diaz E
  Title
Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12.
  Journal
J Bacteriol 179:2573-81 (1997)
DOI:10.1128/JB.179.8.2573-2581.1997
  Sequence
[eco:b0347]
Reference
4  [PMID:9603882]
  Authors
Diaz E, Ferrandez A, Garcia JL.
  Title
Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12.
  Journal
J Bacteriol 180:2915-23 (1998)
DOI:10.1128/JB.180.11.2915-2923.1998
  Sequence
[eco:b0347]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.127
IUBMB Enzyme Nomenclature: 1.14.13.127
ExPASy - ENZYME nomenclature database: 1.14.13.127
BRENDA, the Enzyme Database: 1.14.13.127

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