KEGG   ENZYME: 1.14.13.133Help
Entry
EC 1.14.13.133              Enzyme                                 

Name
pentalenene oxygenase;
PtlI
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
pentalenene,NADPH:oxygen 13-oxidoreductase
Reaction(IUBMB)
pentalenene + 2 NADPH + 2 H+ + 2 O2 = pentalen-13-al + 2 NADP+ + 3 H2O (overall reaction) [RN:R09814];
(1a) pentalenene + NADPH + H+ + O2 = pentalen-13-ol + NADP+ + H2O [RN:R09812];
(1b) pentalen-13-ol + NADPH + H+ + O2 = pentalen-13-al + NADP+ + 2 H2O [RN:R09813]
Reaction(KEGG)
Substrate
pentalenene [CPD:C01841];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
pentalen-13-ol [CPD:C19938]
Product
pentalen-13-al [CPD:C19939];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
pentalen-13-ol [CPD:C19938]
Comment
A heme-thiolate protein (P-450). The enzyme is involved in the biosynthesis of pentalenolactone and related antibiotics.
History
EC 1.14.13.133 created 2011
Pathway
ec00909  Sesquiterpenoid and triterpenoid biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K15907  pentalenene oxygenase
Genes
SMA: SAVERM_2999(ptlI)
SCT: SCAT_p0090(ptlI)
SCY: SCATT_p16540
SBH: SBI_09680(cyp48)
SDV: BN159_1442(ptlI)
SCI: B446_29700
SCW: TU94_27410
SLE: sle_13150(sle_13150)
KSK: KSE_03600
Taxonomy
Reference
1  [PMID:17017767]
  Authors
Quaderer R, Omura S, Ikeda H, Cane DE
  Title
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis.
  Journal
J Am Chem Soc 128:13036-7 (2006)
DOI:10.1021/ja0639214
  Sequence
[sma:SAV_2999]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.133
IUBMB Enzyme Nomenclature: 1.14.13.133
ExPASy - ENZYME nomenclature database: 1.14.13.133
BRENDA, the Enzyme Database: 1.14.13.133

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