KEGG   ENZYME: 1.14.13.172Help
Entry
EC 1.14.13.172              Enzyme                                 

Name
salicylate 5-hydroxylase;
nagG (gene name);
nagH (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
salicylate,NADH:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
salicylate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O [RN:R07709]
Reaction(KEGG)
R07709;
(other) R07710
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Substrate
salicylate [CPD:C00805];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxybenzoate [CPD:C00628];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
This enzyme, which was characterized from the bacterium Ralstonia sp. U2, comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.7, ferredoxin---NAD(P)+ reductase), an iron-sulfur oxygenase, and ferredoxin.
History
EC 1.14.13.172 created 2013
Pathway
ec00626  Naphthalene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K18242  salicylate 5-hydroxylase large subunit
K18243  salicylate 5-hydroxylase small subunit
Genes
EBT: EBL_c34270 EBL_c34280
PLU: plu1000 plu1001
PTT: VY86_00150 VY86_00155
ALT: ambt_18005 ambt_18010
TTU: TERTU_1474 TERTU_1475
MMW: Mmwyl1_2669 Mmwyl1_2670
MME: Marme_2404 Marme_2405
MPC: Mar181_2357 Mar181_2358
GBI: PG2T_03615
RSO: RSc1089(nagH) RSc1090(nagG)
RSE: F504_1060(ohbA) F504_1061(ohbB)
REH: H16_A1632(nagH) H16_A1633(nagG)
CNC: CNE_1c16400(nagH) CNE_1c16410(nagG)
RME: Rmet_3712(nagH)
AXX: ERS451415_00956(andAd_1) ERS451415_00957(andAc_1)
AJS: Ajs_3090
LIM: L103DPR2_01957(nagH) L103DPR2_01958(nagG_2)
LIH: L63ED372_02714(nagH) L63ED372_02715(nagG_2)
AZO: azo2515(nagH) azo2516(nagG)
TMO: TMO_a0553 TMO_a0554(bphA)
ACTI: UA75_12800
ACAD: UA74_12720
AHG: AHOG_11995(nagG)
 » show all
Taxonomy
Reference
1  [PMID:9573207]
  Authors
Fuenmayor SL, Wild M, Boyes AL, Williams PA
  Title
A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2.
  Journal
J Bacteriol 180:2522-30 (1998)
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.172
IUBMB Enzyme Nomenclature: 1.14.13.172
ExPASy - ENZYME nomenclature database: 1.14.13.172
BRENDA, the Enzyme Database: 1.14.13.172

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