KEGG ENZYME: 1.14.13.172

ENZYME: 1.14.13.172

Entry

EC 1.14.13.172              Enzyme

Name

salicylate 5-hydroxylase;
nagG (gene name);
nagH (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

salicylate,NADH:oxygen oxidoreductase (5-hydroxylating)

Reaction(IUBMB)

salicylate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O [RN:R07709]

Reaction(KEGG)

R07709;
(other) R07710

Substrate

salicylate [CPD:C00805];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

2,5-dihydroxybenzoate [CPD:C00628];
NAD+ [CPD:C00003];
H2O [CPD:C00001]

Comment

This enzyme, which was characterized from the bacterium Ralstonia sp. U2, comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.7, ferredoxin---NAD(P)+ reductase), an iron-sulfur oxygenase, and ferredoxin.

History

EC 1.14.13.172 created 2013

Pathway

ec00626

Naphthalene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K18242

salicylate 5-hydroxylase large subunit

K18243

salicylate 5-hydroxylase small subunit

Genes

EBT:	EBL_c34270 EBL_c34280

KAS:	KATP_22590 KATP_22600

KLU:	K7B04_17405 K7B04_17410

PLU:	plu1000 plu1001

PLUM:

A4R40_04995 A4R40_05000

PTT:	VY86_00150 VY86_00155

ALT:	ambt_18005 ambt_18010

TTU:	TERTU_1474 TERTU_1475

CYQ:	Q91_0876 Q91_2234

CZA:	CYCME_2552

CYY:	CPC19_01630

MMW:	Mmwyl1_2669 Mmwyl1_2670

MME:	Marme_2404 Marme_2405

MPC:	Mar181_2357 Mar181_2358

MPRI:

MP3633_2253 MP3633_2254

MARL:

HH196_06880 HH196_06885

FCE:	JN531_005580 JN531_005585

GBI:	PG2T_03615

RSO:	RSc1089(nagH) RSc1090(nagG)

RSE:	F504_1060(ohbA) F504_1061(ohbB)

RPU:	CDC45_05590 CDC45_05595

RNC:	GO999_11020 GO999_11025

RSL:	RPSI07_2301 RPSI07_2302

RSN:	RSPO_c02274 RSPO_c02275(nagH)

RSM:	CMR15_20208 CMR15_20209

RSY:	RSUY_15110 RSUY_15120

RSG:	JK151_19135 JK151_19140

RPJ:	N234_28705 N234_28710

RMN:	TK49_07930 TK49_07935

REH:	H16_A1632(nagH) H16_A1633(nagG)

CNC:	CNE_1c16400(nagH) CNE_1c16410(nagG)

CUH:	BJN34_29220 BJN34_29225

REU:	Reut_B4719 Reut_B4720

RME:	Rmet_3712(nagH)

CBW:	RR42_s2751 RR42_s2752

CCUP:

BKK81_31195 BKK81_31200

CUP:	BKK80_25150 BKK80_25155

CUU:	BKK79_30860 BKK79_30865

COX:	E0W60_10140 E0W60_10145

CCAX:

KZ686_18765 KZ686_18770

CUK:	KB879_08290 KB879_08295

BMK:	DM80_1440 DM80_1441

BCEP:

APZ15_32865 APZ15_32870

BPYR:

ABD05_24780 ABD05_24785

BUB:	BW23_5815 BW23_5816

BARI:

NLX30_26735 NLX30_26740

BSER:

R0290_17330 R0290_17335

BGL:	bglu_2g20110 bglu_2g20120

BGU:	KS03_3913 KS03_3914

BUG:	BC1001_0576 BC1001_0577

BUQ:	AC233_10900 AC233_10905

BURK:

DM992_22160 DM992_22165

BPX:	BUPH_04466 BUPH_04467

BUZ:	AYM40_33040 AYM40_33045 AYM40_33150 AYM40_33155

BFN:	OI25_3046 OI25_3047

PARB:

CJU94_38670 CJU94_38675

PTER:

C2L65_39070 C2L65_39075

PMEG:

FNZ07_23540 FNZ07_23545 FNZ07_29070 FNZ07_29075

PPAI:

E1956_38420 E1956_38425

POLY:

PSHI2_07660 PSHI2_07670 PSHI2_13350 PSHI2_13360

POLN:

PSHI8_07660 PSHI8_07670 PSHI8_13350 PSHI8_13360

PPK:	U875_04295 U875_04300

PPNO:

DA70_21370 DA70_21375

PPNM:

LV28_08945 LV28_08950

PRB:	X636_05145 X636_05150

PPUL:

RO07_03175 RO07_03180

PSPU:

NA29_24085 NA29_24090

PAPI:

SG18_03365 SG18_03370

PVE:	UC34_19330 UC34_19335

POX:	MB84_24085 MB84_24090

PFG:	AB870_06325 AB870_06330

PNR:	AT302_02760 AT302_02765

PAND:

DRB87_17855 DRB87_17860

PFIB:

PI93_008710 PI93_008715

PCOM:

NTU39_21090 NTU39_21095

PANN:

PanNE5_24100 PanNE5_24110

PANX:

OYT13_19450 OYT13_19455

CABK:

NK8_48060 NK8_48070

BPT:	Bpet2804 Bpet2805

AXY:	AXYL_00938 AXYL_00939 AXYL_06578 AXYL_06581(bphA)

AXO:	NH44784_011911 NH44784_011921

AXN:	AX27061_0965 AX27061_0966

AXX:	ERS451415_00956(andAd_1) ERS451415_00957(andAc_1)

ADT:	APT56_05010 APT56_05015

AIS:	BUW96_21015 BUW96_21020

ASW:	CVS48_01680 CVS48_01685

ACHR:

C2U31_09370

ACHB:

DVB37_04180 DVB37_04185

ADY:	HLG70_17185 HLG70_17190

ACHO:

H4P35_04190 H4P35_04195

AMUI:

PE062_01190 PE062_01195

APES:

FOC84_07680 FOC84_07685

AAEG:

RA224_27665 RA224_27670

ASEL:

RAS12_17385 RAS12_17390

PUD:	G9Q38_01795 G9Q38_01800 G9Q38_05245 G9Q38_05250

PIG:	EGT29_23975 EGT29_23980

PARK:

LSG25_18560 LSG25_18565

NARM:

N7E01_10645

RHF:	EUB48_13715 EUB48_13720

POL:	Bpro_0983 Bpro_0984

PNA:	Pnap_2462 Pnap_2487 Pnap_2488

POS:	DT070_14060 DT070_14065

POO:	F7R28_16540 F7R28_16545

AJS:

Ajs_3090

ACK:	C380_09995 C380_10000

ACIQ:

ACDW_45530 ACDW_45540

ACIV:

EXV95_08450 EXV95_08455

AAV:	Aave_0165 Aave_0166

AAA:	Acav_0196 Acav_0197

VAP:	Vapar_5348 Vapar_5349

VPE:	Varpa_2933 Varpa_2934

VPD:	VAPA_1c07030 VAPA_1c07040 VAPA_1c20400 VAPA_1c20410

VBO:	CKY39_17840 CKY39_17845

VAM:	C4F17_05690 C4F17_05695

VAZ:	H7F35_31265 H7F35_31270

VAV:	IG196_15110 IG196_15115

RTA:	Rta_25980 Rta_25990

RTEA:

HK414_18880 HK414_18885

LIM:	L103DPR2_01957(nagH) L103DPR2_01958(nagG_2)

LIH:	L63ED372_02714(nagH) L63ED372_02715(nagG_2)

HYR:	BSY239_1027 BSY239_1029

HYB:	Q5W_19150 Q5W_19155

HYL:	LPB072_20440 LPB072_20445

HPSE:

HPF_02890(nagG) HPF_02895(nagH)

HYN:	F9K07_14335 F9K07_14340

HTN:	KI616_03135 KI616_03140

HCZ:	G9Q37_10175 G9Q37_10180

HYY:	JNX00_12195 JNX00_12200 JNX00_19110 JNX00_19115

ATER:

MW290_00170 MW290_00175

LCH:	Lcho_0796 Lcho_0797

JAJ:	EKL02_16425 EKL02_16430

HEW:	HBDW_22680 HBDW_22690

MASW:

AM586_27630 AM586_27635 AM586_27695 AM586_27700 AM586_27790 AM586_27795

UND:	UNDKW_4640 UNDKW_4641

AZO:	azo2515(nagH) azo2516(nagG)

AOA:	dqs_2672 dqs_2673

AZR:	CJ010_15880 CJ010_15885

CFH:	C1707_18700 C1707_18705

BPON:

IFE19_06475 IFE19_06480

BBUL:

U0030_10915 U0030_10920

NAR:

Saro_3834

NPP:	PP1Y_AT15862

SWI:	Swit_0895 Swit_3056 Swit_3057

SPHD:

HY78_10290 HY78_10295 HY78_22215

SBAR:

H5V43_22430

SLAA:

EUU25_00835 EUU25_00840

BLAS:

BSY18_3955 BSY18_3956

PALG:

HFP57_01960 HFP57_01965

AZL:	AZL_a09200 AZL_a09210

ALI:	AZOLI_p20648 AZOLI_p20649

AZS:	E6C72_28690 E6C72_28695

FER:	FNB15_08990 FNB15_08995

TMO:	TMO_a0553 TMO_a0554(bphA)

NFR:	ERS450000_00310(andAd)

RHOJ:

JVH1_39340

AGRA:

AGRA3207_001975

ACTI:

UA75_12800

ACAD:

UA74_12720

AHG:	AHOG_11995(nagG)

LEPV:

J4558_27375

VAB:	WPS_05620 WPS_05630

» show all

Reference

1 [PMID:9573207]

Authors

Fuenmayor SL, Wild M, Boyes AL, Williams PA

Title

A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2.

Journal

J Bacteriol 180:2522-30 (1998)
DOI:10.1128/JB.180.9.2522-2530.1998

Sequence

[ag:AAD12607 AAD12608]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.172

IUBMB Enzyme Nomenclature:

1.14.13.172

ExPASy - ENZYME nomenclature database:

1.14.13.172

BRENDA, the Enzyme Database:

1.14.13.172

DBGET integrated database retrieval system