KEGG   ENZYME: 1.14.13.175Help
Entry
EC 1.14.13.175              Enzyme                                 

Name
aflatoxin B synthase;
ordA (gene name);
8-O-methylsterigmatocystin,NADPH:O2 oxidoreductase (aflatoxin-B forming)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
8-O-methylsterigmatocystin,NADPH:oxygen oxidoreductase (aflatoxin-B forming)
Reaction(IUBMB)
(1) 8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2 = aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2 [RN:R10319];
(2) 8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2 = aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2 [RN:R10320]
Reaction(KEGG)
Substrate
8-O-methylsterigmatocystin [CPD:C03686];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
8-O-methyldihydrosterigmatocystin [CPD:C03944]
Product
aflatoxin B1 [CPD:C06800];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
methanol [CPD:C00132];
CO2 [CPD:C00011];
aflatoxin B2 [CPD:C16753]
Comment
A heme-thiolate (P-450) enzyme. Isolated from the mold Aspergillus parasiticus.
History
EC 1.14.13.175 created 2013
Pathway
ec00254  Aflatoxin biosynthesis
Orthology
K17651  aflatoxin B synthase
Genes
AOR: AO090026000027
AFV: AFLA_139200
Taxonomy
Reference
1  [PMID:1902378]
  Authors
Bhatnagar D, Cleveland TE, Kingston DG
  Title
Enzymological evidence for separate pathways for aflatoxin B1 and B2 biosynthesis.
  Journal
Biochemistry 30:4343-50 (1991)
Reference
2  [PMID:9835571]
  Authors
Yu J, Chang PK, Ehrlich KC, Cary JW, Montalbano B, Dyer JM, Bhatnagar D, Cleveland TE
  Title
Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2.
  Journal
Appl Environ Microbiol 64:4834-41 (1998)
  Sequence
Reference
3  [PMID:11996570]
  Authors
Udwary DW, Casillas LK, Townsend CA
  Title
Synthesis of 11-hydroxyl O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis.
  Journal
J Am Chem Soc 124:5294-303 (2002)
DOI:10.1021/ja012185v
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.175
IUBMB Enzyme Nomenclature: 1.14.13.175
ExPASy - ENZYME nomenclature database: 1.14.13.175
BRENDA, the Enzyme Database: 1.14.13.175

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