KEGG   ENZYME: 1.14.13.186Help
Entry
EC 1.14.13.186              Enzyme                                 

Name
20-oxo-5-O-mycaminosyltylactone 23-monooxygenase;
tylH1 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
20-oxo-5-O-beta-mycaminosyltylactone,NADPH:oxygen oxidoreductase (23-hydroxylating)
Reaction(IUBMB)
20-oxo-5-O-beta-mycaminosyltylactone + NADPH + H+ + O2 = 5-O-beta-mycaminosyltylonolide + NADP+ + H2O [RN:R10656]
Reaction(KEGG)
Substrate
20-oxo-5-O-beta-mycaminosyltylactone [CPD:C20760];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
5-O-beta-mycaminosyltylonolide;
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A cytochrome P-450 heme-thiolate enzyme. Involved in the biosynthetic pathway of the macrolide antibiotic tylosin, which is produced by several species of Streptomyces bacteria.
History
EC 1.14.13.186 created 2014
Pathway
ec00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ec01130  Biosynthesis of antibiotics
Orthology
K15991  20-oxo-5-O-mycaminosyltylactone 23-monooxygenase
Reference
1  [PMID:7283418]
  Authors
Baltz RH, Seno ET
  Title
Properties of Streptomyces fradiae mutants blocked in biosynthesis of the macrolide antibiotic tylosin.
  Journal
Antimicrob Agents Chemother 20:214-25 (1981)
  Sequence
Reference
2  [PMID:15489173]
  Authors
Reeves CD, Ward SL, Revill WP, Suzuki H, Marcus M, Petrakovsky OV, Marquez S, Fu H, Dong SD, Katz L
  Title
Production of hybrid 16-membered macrolides by expressing combinations of polyketide synthase genes in engineered Streptomyces fradiae hosts.
  Journal
Chem Biol 11:1465-72 (2004)
DOI:10.1016/j.chembiol.2004.08.019
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.186
IUBMB Enzyme Nomenclature: 1.14.13.186
ExPASy - ENZYME nomenclature database: 1.14.13.186
BRENDA, the Enzyme Database: 1.14.13.186

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