Entry
Name
4-hydroxybenzoate 3-monooxygenase;
p-hydroxybenzoate hydrolyase;
p-hydroxybenzoate hydroxylase;
4-hydroxybenzoate 3-hydroxylase;
4-hydroxybenzoate monooxygenase;
4-hydroxybenzoic hydroxylase;
p-hydroxybenzoate-3-hydroxylase;
p-hydroxybenzoic acid hydrolase;
p-hydroxybenzoic acid hydroxylase;
p-hydroxybenzoic hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
4-hydroxybenzoate,NADPH:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
4-hydroxybenzoate + NADPH + H+ + O2 = 3,4-dihydroxybenzoate + NADP+ + H2O [RN:
R01298 ]
Reaction(KEGG)
Substrate
Product
Comment
A flavoprotein (FAD). Most enzymes from Pseudomonas are highly specific for NADPH (cf. EC
1.14.13.33 4-hydroxybenzoate 3-monooxygenase [NAD(P)H]).
History
EC 1.14.13.2 created 1972, modified 1999
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
K00481 p-hydroxybenzoate 3-monooxygenase
Genes
REE : electrica_04361(pobA)
RTG : NCTC13098_06382(pobA)
KIE : NCTC12125_03101(pobA)
SFJ : SAMEA4384070_2556(pobA)
XPH : XppCFBP6546_10040(pobA)
XHD : LMG31886_03980(pobA)
VIB : VspSTUT11_39090(pobA)
PAEB : NCGM1900_0238(pobA)
PNT : G5B91_06935(pobA) G5B91_12885(pobA)
PVD : CFBP1590__1782(pobA)
PPRC : PFLCHA0_c51300(pobA)
PMUD : NCTC8068_01387(pobA)
PFW : PF1751_v1c14910(pobA)
PBC : CD58_06740 CD58_18720
PPUU : PputUW4_04363(pobA)
PTRT : HU722_0008540(pobA)
PASG : KSS96_08180(pobA) KSS96_11245(pobA)
PTW : TUM18999_24630(pobA)
PJU : L1P09_13820(pobA) L1P09_15200(pobA)
PKM : PZ739_15600(pobA) PZ739_27655(pobA)
GAI : IMCC3135_15590(pobA)
HAK : KO116_00173 KO116_00196
HAAH : HALA3H3_370022(pobA)
CNC : CNE_2c22540(pobA) CNE_BB1p04760(pobA)
REU : Reut_B4006 Reut_B5020
RME : Rmet_4018(pobA) Rmet_5490(pobA)
CNAN : A2G96_29575 A2G96_30415
BMN : BMA10247_A0055(pobA)
BPM : BURPS1710b_A1551(pobA)
BPL : BURPS1106A_A0053(pobA)
BPD : BURPS668_A0066(pobA)
BPZ : BP1026B_II0043(pobA)
BCEP : APZ15_25430 APZ15_29790
BURK : DM992_18230 DM992_26235(pobA)
BCAI : K788_0006322 K788_0007693
PHS : C2L64_19175 C2L64_19780
PTER : C2L65_17050 C2L65_17645
PMEG : FNZ07_03310 FNZ07_14630
PSAA : QEN71_15625(pobA) QEN71_18085
PLAD : PPGU16_45870(pobA_1) PPGU16_47050(pobA_2)
PLG : NCTC10937_03379(pobA)
CABK : NK8_35500 NK8_73210
TVL : FAZ95_14815 FAZ95_22195(pobA)
BOJ : CBF45_11670 CBF45_11910
CDN : BN940_06651 BN940_08471
PIG : EGT29_01995 EGT29_05815(pobA) EGT29_16585
KGY : EHF36_11925(pobA) EHF36_12475(pobA)
RHF : EUB48_07550 EUB48_11140(pobA)
ACK : C380_01240 C380_19695
ACIQ : ACDW_02900(pobA) ACDW_34420(pobA)
ACIV : EXV95_11715(pobA) EXV95_17765(pobA)
CTT : CtCNB1_2156 CtCNB1_2416
LIM : L103DPR2_01960(pobA_2)
LIH : L63ED372_01799(pobA)
HYL : LPB072_07785 LPB072_12310
HYN : F9K07_14030(pobA) F9K07_15800
KIA : G8A07_08935(pobA) G8A07_24925(pobA)
SMIO : CATMQ487_07430(pobA)
URU : DSM104443_02508(praI)
SMEL : SM2011_b20583(pobA)
SFD : USDA257_c09700(pobA)
SAME : SAMCFNEI73_pC0867(pobA)
ALF : CFBP5473_15900(pobA)
AVV : RvVAT039_42990(pobA)
AVF : RvVAR031_35580(pobA)
ASAL : CFBP5507_16655(pobA)
RPUS : CFBP5875_14925(pobA)
ACUC : KZ699_22715(pobA) KZ699_24905(pobA)
ALEG : CFBP4996_24070(pobA)
REC : RHECIAT_CH0003682(pobA)
REL : REMIM1_CH03508(pobA)
REP : IE4803_CH03747(pobA)
REI : IE4771_CH03712(pobA)
RTR : RTCIAT899_PC07405(pobA)
RHL : LPU83_pLPU83d0110(pobA)
RGA : RGR602_PC00883(pobA)
RPHA : AMC79_CH03604(pobA)
RLW : RlegWSM1455_17585(pobA)
RBW : RLCC275e_17800(pobA)
BSW : IY71_00410 IY71_14260
BIO : BR141012304_20378(pobA)
BJA : bll7838(pobA) blr2337(pobA) blr6420(pobA)
BJU : BJ6T_75040 BJ6T_84600
BJP : RN69_36400 RN69_40880
BRS : S23_09490(pobA) S23_56910(pobA)
BRC : BCCGELA001_28325 BCCGELA001_32980
BRAD : BF49_2608 BF49_4153
BIC : LMTR13_35535 LMTR13_36400
BRK : CWS35_03005(pobA) CWS35_38425
BOT : CIT37_01360(pobA) CIT37_06540(pobA)
BRQ : CIT40_05940(pobA) CIT40_28150(pobA)
BGQ : X265_29520(pobA) X265_33245(pobA)
BGZ : XH91_27870(pobA) XH91_31640(pobA)
BSYM : CIT39_04575(pobA) CIT39_27155(pobA)
BBET : F8237_08240(pobA) F8237_19665(pobA) F8237_35200
BARH : WN72_05990(pobA) WN72_11655(pobA)
BVZ : BRAD3257_6910(pobA) BRAD3257_8260(pobA)
BDG : LPJ38_00655(pobA) LPJ38_34535(pobA)
BSEP : HAP48_0018975(pobA)
BSEI : KMZ68_23785(pobA) KMZ68_25400(pobA)
BCAN : BcanWSM471_05735(pobA) BcanWSM471_09820(pobA)
BCOU : IC761_28040(pobA) IC761_33270(pobA)
BPAH : QA639_12800 QA639_39435(pobA)
BXN : I3J27_07510(pobA) I3J27_36035(pobA)
BRAZ : LRP30_08910(pobA) LRP30_39425(pobA)
BRAY : ML401_06595(pobA) ML401_30625(pobA)
RHOL : RBJ75_05610 RBJ75_11385(pobA)
VGO : GJW-30_1_00442(pobA)
BVV : BHK69_10390 BHK69_29830
BVES : QO058_29060(pobA) QO058_29385
BOSE : RMR04_00445 RMR04_24040(pobA)
MAQU : Maq22A_c05395(ubiH)
RHZ : RHPLAN_00710 RHPLAN_43330
DEQ : XM25_06525 XM25_18595
YTI : FNA67_00245 FNA67_10640(pobA)
MHAR : L1P08_06825(pobA) L1P08_14260(pobA)
PSIN : CAK95_24850 CAK95_25310
PSTG : E8M01_02535 E8M01_13665(pobA)
RLI : RLO149_c021620(pobA)
PHP : PhaeoP97_03078(pobA)
PPIC : PhaeoP14_00199(pobA)
LEJ : ETW24_03645(pobA) ETW24_22650
LEV : ETW23_16550(pobA) ETW23_23395
MALG : MALG_02279 MALG_04243
SPSE : SULPSESMR1_03390(pobA)
AHT : ANTHELSMS3_00715(pobA)
LALG : LentiSH36_02394(pobA)
PARO : CUV01_04085(pobA) CUV01_09785(pobA)
PAEX : JHW48_16335(pobA) JHW48_17395(pobA)
PGV : SL003B_2722 SL003B_3140
NOV : TQ38_019270(pobA) TQ38_028375(pobA)
SBIN : SBA_ch2_7500 SBA_pBAR1_670
SYA : A6768_02815(pobA) A6768_12975
SPAG : sphantq_03919(pobA)
SFLA : SPHFLASMR4Y_00330(pobA)
BSON : S101395_00396(pobA)
CCYS : SAMEA4530656_0405(pobA)
NBR : O3I_021790 O3I_022545
NAD : NCTC11293_02937(pobA)
NYA : LTV02_34305 LTV02_37230
RGO : KYT97_13010 KYT97_16500
GPO : GPOL_c02570(pobA1) GPOL_c40540(pobA2)
GOM : D7316_02312(pobA) D7316_03129(praI)
GAM : GII34_05725 GII34_12225
GOI : LK459_04825 LK459_08665
SCO : SCO1308(SCBAC36F5.19) SCO3084(pobA)
SGRF : SGFS_010650 SGFS_097370 SGFS_102580
SCB : SCAB_11121 SCAB_84531(pobA) SCAB_88861
SVL : Strvi_0432 Strvi_2305
SHO : SHJGH_2493 SHJGH_4319
SRC : M271_14295 M271_20520 M271_38015
SMAL : SMALA_1621 SMALA_5898
SSOI : I1A49_11245 I1A49_33325 I1A49_43185
SDV : BN159_1192(pobA1) BN159_5194(pobA3) BN159_7296
SALS : SLNWT_0030 SLNWT_0892
SLV : SLIV_22275(pobA) SLIV_31250
STRE : GZL_02696 GZL_07357
SCW : TU94_05270 TU94_13515
SLC : SL103_12990 SL103_25665
STRC : AA958_17210 AA958_29240 AA958_32565
SAMB : SAM23877_3136(pobA)
SCX : AS200_06985 AS200_26970 AS200_30505 AS200_37255
SRW : TUE45_01686(pobA_1) TUE45_04574(pobA_2)
STRF : ASR50_06215 ASR50_19865
SLE : sle_01860(sle_01860) sle_41900(sle_41900) sle_57900(sle_57900)
SPAV : Spa2297_04795 Spa2297_13400
STSI : A4E84_06360 A4E84_16225
SGM : GCM10017557_32060 GCM10017557_48650 GCM10017557_80270(pobA)
SCAD : DN051_23190 DN051_32495
SPHW : NFX46_26940 NFX46_36295
SLS : SLINC_1488 SLINC_3509
SNR : SNOUR_11565 SNOUR_33840
SALU : DC74_1785 DC74_6312
SALL : SAZ_09505 SAZ_31695
SAUO : BV401_11570 BV401_33385
SPUN : BFF78_24905 BFF78_35215
SALF : SMD44_03437(pobA) SMD44_04582(pobA) SMD44_08597(pobA)
STRO : STRMOE7_07095 STRMOE7_29935
SNZ : DC008_04625 DC008_31000
SGE : DWG14_00771(praI_2) DWG14_01080(pobA_3) DWG14_04983(praI_3) DWG14_05369(pobA_4) DWG14_07188(praI_4)
SDX : C4B68_02855 C4B68_03710
SGD : ELQ87_07850 ELQ87_23030 ELQ87_27220 ELQ87_33330
SCYA : EJ357_04010 EJ357_07175 EJ357_18625
SAST : CD934_19910 CD934_29235
SKA : CP970_03505 CP970_25950
SHAW : CEB94_06815 CEB94_16820
SGAL : CP966_04795 CP966_14755
SFY : GFH48_02180 GFH48_04755 GFH48_24070
SAQU : EJC51_09000 EJC51_21065 EJC51_40875
SBY : H7H31_00470 H7H31_12515
SRIM : CP984_01830 CP984_08110
SPHV : F9278_02370 F9278_25795 F9278_43770
SPAC : B1H29_22080 B1H29_31060
SCHA : CP983_03860 CP983_20365 CP983_25740 CP983_36800
SGX : H4W23_19770 H4W23_37180
SCOE : CP976_06985 CP976_18040
SBAT : G4Z16_00490 G4Z16_05360 G4Z16_14555
SSPB : CP982_01965 CP982_02335 CP982_18225
SPLA : CP981_05700 CP981_30210
SBRO : GQF42_09035 GQF42_19145 GQF42_36910
STUI : GCM10017668_08840 GCM10017668_27970
SCHF : IPT68_05605 IPT68_14970
SFEU : IM697_05535 IM697_16260 IM697_19855
SDW : K7C20_05310 K7C20_28920
SMOB : J7W19_02340 J7W19_07290 J7W19_25015 J7W19_29985
SPRA : CP972_02445 CP972_05665
SROI : IAG44_07525 IAG44_24585 IAG44_34060
SANU : K7396_00645 K7396_30440
SINE : KI385_08380 KI385_34630
SLF : JEQ17_07265 JEQ17_19240 JEQ17_20900 JEQ17_27420
SDEC : L3078_03045 L3078_24505 L3078_41690
SAOV : G3H79_17310 G3H79_22040
SDUR : M4V62_25120 M4V62_39265
SAKB : K1J60_02030 K1J60_20595 K1J60_41990
STAA : LDH80_35510 LDH80_37555
SNIG : HEK616_22400 HEK616_49710
SCAE : IHE65_02990 IHE65_21170 IHE65_42320
SSPN : LXH13_05920 LXH13_24265 LXH13_35400
SCIR : STRCI_001214 STRCI_003448 STRCI_007246
SXT : KPP03845_100300(praI)
SLON : LGI35_05015 LGI35_19915
STEE : F3L20_11250 F3L20_28690
SENG : OJ254_21875 OJ254_29595
SYUN : MOV08_11355 MOV08_34075
SARG : HKX69_20420 HKX69_29705
SJN : RI060_25315 RI060_36035
SCYN : N8I84_16970 N8I84_37840
SKG : KJK29_17455 KJK29_22570 KJK29_32890
SCOA : QU709_25430 QU709_37230
SFP : QUY26_18505 QUY26_23695
SINN : ABB07_22460 ABB07_32340
STRZ : OYE22_00545 OYE22_12290
KBU : Q4V64_07595 Q4V64_20615
SARM : DVA86_14775 DVA86_15280
SPRF : K4G22_14050 K4G22_30170
SLUO : PS467_25085 PS467_30820
STPB : QR97_19125 QR97_19845
SRPA : HEP86_05855 HEP86_07185 HEP86_31450
SCAJ : O1G22_25025 O1G22_35365
STRL : HEP84_22330 HEP84_45055 HEP84_48005
SRPZ : HEP85_17095 HEP85_34330 HEP85_36340
KSK : KSE_55520(pobA2) KSE_75180(pobA1)
KIT : CFP65_0444 CFP65_5583
KCH : O1G21_04985 O1G21_06735
GYU : FE374_09870 FE374_17135
RAIN : Rai3103_09035 Rai3103_09195
NAQU : ENKNEFLB_00527(praI)
AGRA : AGRA3207_000019 AGRA3207_002290
ACTQ : OG417_15205 OG417_33385
NOA : BKM31_02955 BKM31_48070
NGN : LCN96_39135(pobA) LCN96_53945
NFS : OIE67_09555 OIE67_49825
SEN : SACE_0822(pobA) SACE_4035(pobA)
AMD : AMED_0392(pobA) AMED_1292(pobA) AMED_7579(pobA)
AMN : RAM_02010 RAM_06555 RAM_38940
AMM : AMES_0392(pobA) AMES_1283(pobA) AMES_7466(pobA)
AMZ : B737_0393(pobA) B737_1284(pobA) B737_7466(pobA)
AOI : AORI_1307(pobA) AORI_3518(pobA)
AJA : AJAP_19040 AJAP_21540(pobA) AJAP_33025
AMYB : BKN51_12545 BKN51_14895 BKN51_40635
AORI : SD37_18180 SD37_34660
APRT : MUY14_11415 MUY14_46445
AROO : NQK81_04920 NQK81_16325
ATHM : L1857_00325(pobA) L1857_16880
AMOG : QRX60_21720 QRX60_40370
ACOR : LCL61_24840 LCL61_27115 LCL61_38130
AMYZ : HUW46_04703(praI_1) HUW46_08334(praI_2) HUW46_08445(pobA)
PBRO : HOP40_01510 HOP40_32895
PSDS : K1T35_08225 K1T35_42990
SESP : BN6_35050 BN6_41680(pobA1) BN6_65580(pobA3)
SSYI : EKG83_20725 EKG83_37205
SACX : IOD16_12505 IOD16_26680
KAL : KALB_2911 KALB_5788 KALB_6506
KUT : JJ691_27890(pobA) JJ691_38230
KPHY : AOZ06_34190 AOZ06_42375
LED : BBK82_24885 BBK82_43435
CROS : N8J89_15160 N8J89_22950
ACTY : OG774_14415 OG774_16670
UME : RM788_02215 RM788_14160 RM788_18160 RM788_51000
ACTN : L083_3090 L083_8052(pobA)
ASIC : Q0Z83_059680 Q0Z83_060990
PFLA : Pflav_051720 Pflav_074380(pobA)
BALA : DSM104299_03149(praI)
SBAE : DSM104329_00839(praI)
MPRO : BJP34_11765 BJP34_12140
KBS : EPA93_29000 EPA93_45860
» show all
Taxonomy
Reference
Authors
Hosokawa K, Stanier RY.
Title
Crystallization and properties of p-hydroxybenzoate hydroxylase from Pseudomonas putida.
Journal
J Biol Chem 241:2453-60 (1966)
Reference
Authors
Howell LG, Spector T, Massey V.
Title
Purification and properties of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.
Journal
J Biol Chem 247:4340-50 (1972)
Reference
Authors
Spector T, Massey V.
Title
Studies on the effector specificity of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.
Journal
J Biol Chem 247:4679-87 (1972)
Reference
Authors
Spector T, Massey V.
Title
p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Evidence for an oxygenated flavin intermediate.
Journal
J Biol Chem 247:5632-6 (1972)
Reference
Authors
Spector T, Massey V.
Title
p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Reactivity with oxygen.
Journal
J Biol Chem 247:7123-7 (1972)
Reference
Authors
Seibold B, Matthes M, Eppink MH, Lingens F, Van Berkel WJ, Muller R
Title
4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 1.14.13.2
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.2