KEGG   ENZYME: 1.14.13.208Help
Entry
EC 1.14.13.208              Enzyme                                 

Name
benzoyl-CoA 2,3-epoxidase;
benzoyl-CoA dioxygenase/reductase (incorrect);
BoxBA;
BoxA/BoxB system;
benzoyl-CoA 2,3-dioxygenase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
benzoyl-CoA,NADPH:oxygen oxidoreductase (2,3-epoxydizing)
Reaction(IUBMB)
benzoyl-CoA + NADPH + H+ + O2 = 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O [RN:R09555]
Reaction(KEGG)
Substrate
benzoyl-CoA [CPD:C00512];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,3-epoxy-2,3-dihydrobenzoyl-CoA [CPD:C19684];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii. BoxB functions as the oxygenase part of benzoyl-CoA oxygenase in conjunction with BoxA, the reductase component, which upon binding of benzoyl-CoA, transfers two electrons to the ring in the course of monooxygenation. BoxA is a homodimeric 46 kDa iron-sulfur-flavoprotein (FAD), BoxB is a monomeric iron-protein [1].
History
EC 1.14.13.208 created 2010 as EC 1.14.12.21, transferred 2015 to EC 1.14.13.208
Pathway
ec00362  Benzoate degradation
Orthology
K15511  benzoyl-CoA 2,3-epoxidase subunit A
K15512  benzoyl-CoA 2,3-epoxidase subunit B
Genes
XAL: XALC_1517(albXII)
ADI: B5T_02165 B5T_02166
AXE: P40_10385 P40_10390
VFF: VITFI_CDS1569 VITFI_CDS1589 VITFI_CDS1590
RPJ: N234_07040 N234_07045 N234_31275 N234_31280
REU: Reut_A1323 Reut_A1324
REH: H16_A1408(bdxA) H16_B1912(boxA) H16_B1913(boxB)
CNC: CNE_1c14360(boxA) CNE_1c14370(boxB) CNE_2c18730(boxA) CNE_2c18740(boxB) CNE_BB1p04280(boxA)
CUH: BJN34_07180 BJN34_07185 BJN34_32090 BJN34_32095
RME: Rmet_1220(boxA) Rmet_1221(boxB)
CTI: RALTA_A1321(bdxA) RALTA_A1322(bdxB) RALTA_B1611(boxA) RALTA_B1612(boxB)
CGD: CR3_3379(boxB) CR3_3380(boxA)
CPAU: EHF44_15400(boxB) EHF44_15405(boxA)
COX: E0W60_03165(boxA) E0W60_03170(boxB) E0W60_16990(boxA) E0W60_16995(boxB)
BXE: Bxe_A1423(boxB) Bxe_C0890(boxA) Bxe_C0891(boxB)
BXB: DR64_3587(boxB) DR64_3588(boxA) DR64_7559(boxB) DR64_7560(boxA)
BFN: OI25_2716(boxB) OI25_2717(boxA)
PARB: CJU94_11400(boxB) CJU94_11405(boxA)
PHS: C2L64_05440(boxB) C2L64_05445(boxA)
PTER: C2L65_05225(boxB) C2L65_05230(boxA)
PGP: CUJ91_12135(boxA) CUJ91_12140(boxB)
PCJ: CUJ87_05135(boxA)
PTS: CUJ90_12200(boxA) CUJ90_12205(boxB)
PAND: DRB87_15890(boxB)
BPT: Bpet3568(petH) Bpet3569(pbcA)
BHO: D560_2021(boxB) D560_2022(boxA)
BHM: D558_2006(boxB) D558_2007(boxA)
BHZ: ACR54_04083(boxA) ACR54_04084(boxB)
AXY: AXYL_05030(boxA) AXYL_05031(boxB)
ASW: CVS48_13535(boxB) CVS48_13540(boxA)
ACHB: DVB37_21270(boxA) DVB37_21275(boxB)
PUS: CKA81_01055(boxB) CKA81_01060(boxA)
AMIM: MIM_c27160(boxA) MIM_c27170(boxB)
PIG: EGT29_17285(boxA) EGT29_17290(boxB)
RHF: EUB48_04950(boxB) EUB48_04955(boxA)
POS: DT070_17675(boxB)
VPD: VAPA_1c00960(boxB) VAPA_1c00970(boxA)
VBO: CKY39_00545(boxB) CKY39_00550(boxA)
VAM: C4F17_18550(boxA) C4F17_18555(boxB)
RTA: Rta_22300
LIM: L103DPR2_00830(boxB) L103DPR2_00831(boxA)
LIH: L63ED372_02245(boxA) L63ED372_02246(boxB)
HYR: BSY239_635(boxB) BSY239_636(boxA)
HYC: E5678_00695(boxB) E5678_00700(boxA)
HPSE: HPF_06705(boxB) HPF_06710(boxA)
CBAA: SRAA_0585(boxB) SRAA_0586(boxA)
THI: THI_0466(boxA) THI_0467(boxB)
SNN: EWH46_00460(boxB) EWH46_00465(boxA)
EBA: ebA2765(boxB) ebA2766(boxA)
AZO: azo3057(boxB) azo3058(boxA)
AZA: AZKH_4519(boxB) AZKH_4520(boXA)
AZD: CDA09_22740(boxB) CDA09_22745(boxA)
AZR: CJ010_18910(boxB) CJ010_18915(boxA)
SUR: STAUR_7626(boxB)
SCL: sce1060(yfcX)
HOH: Hoch_5225
RPOD: E0E05_11135(boxA) E0E05_11140(boxB)
ABAW: D5400_06470(boxB) D5400_06475(boxA)
BJA: blr1081
BRA: BRADO6788(boxB)
BBT: BBta_0750(boxB) BBta_6638(boxA) BBta_6639(boxB)
BRS: S23_67350
AOL: S58_06720
BRO: BRAD285_6936(boxB)
BRK: CWS35_10185(boxB)
BOT: CIT37_08870(boxB)
BRQ: CIT40_30010(boxB)
BGQ: X265_34635(boxB)
BGZ: XH91_33020(boxB)
RPA: RPA0677
RPB: RPB_4653
RPD: RPD_0881
RPE: RPE_0584
RPT: Rpal_0744
VGO: GJW-30_1_01040(boxB) GJW-30_1_01041(boxA)
RBM: TEF_07960
SIL: SPO3701(boxB) SPO3703(boxA)
JAN: Jann_0672(boxB)
PZH: CX676_12415(boxA)
PTP: RCA23_c19060(boxA) RCA23_c19070(boxB)
SULZ: C1J03_02545(boxB) C1J03_02555(boxA)
RID: RIdsm_00626(boxB) RIdsm_00628(boxA)
SAGU: CDO87_17735(boxB) CDO87_17745(boxA)
THAA: CFI11_22010(boxB)
SEDI: EBB79_19920(boxA) EBB79_19925(boxB)
OCD: FHY55_19195(boxA) FHY55_19200(boxB)
MAGX: XM1_1070(boxB) XM1_1071(boxA)
FER: FNB15_18640(boxB)
TOY: FO059_00815(boxB)
JLI: EXU32_11305(boxB)
BSD: BLASA_2556(boxB)
AMD: AMED_8610
AMN: RAM_44190
AMM: AMES_8479
AMZ: B737_8480
AOI: AORI_7396
AJA: AJAP_02020(boxB)
AMYC: CU254_35735(boxB)
PDX: Psed_1489
PSEA: WY02_22245
PSEE: FRP1_20445
PSEH: XF36_05450
PAUT: Pdca_13940
CAI: Caci_2007
 » show all
Taxonomy
Reference
1  [PMID:15458418]
  Authors
Zaar A, Gescher J, Eisenreich W, Bacher A, Fuchs G
  Title
New enzymes involved in aerobic benzoate metabolism in Azoarcus evansii.
  Journal
Mol Microbiol 54:223-38 (2004)
DOI:10.1111/j.1365-2958.2004.04263.x
  Sequence
Reference
2  [PMID:12399500]
  Authors
Gescher J, Zaar A, Mohamed M, Schagger H, Fuchs G
  Title
Genes coding for a new pathway of aerobic benzoate metabolism in Azoarcus evansii.
  Journal
J Bacteriol 184:6301-15 (2002)
DOI:10.1128/JB.184.22.6301-6315.2002
  Sequence
Reference
3  [PMID:11222587]
  Authors
Mohamed ME, Zaar A, Ebenau-Jehle C, Fuchs G
  Title
Reinvestigation of a new type of aerobic benzoate metabolism in the proteobacterium Azoarcus evansii.
  Journal
J Bacteriol 183:1899-908 (2001)
DOI:10.1128/JB.183.6.1899-1908.2001
Reference
4  [PMID:20452977]
  Authors
Rather LJ, Knapp B, Haehnel W, Fuchs G
  Title
Coenzyme A-dependent aerobic metabolism of benzoate via epoxide formation.
  Journal
J Biol Chem 285:20615-24 (2010)
DOI:10.1074/jbc.M110.124156
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.208
IUBMB Enzyme Nomenclature: 1.14.13.208
ExPASy - ENZYME nomenclature database: 1.14.13.208
BRENDA, the Enzyme Database: 1.14.13.208

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