KEGG   ENZYME: 1.14.13.210Help
Entry
EC 1.14.13.210              Enzyme                                 

Name
4-methyl-5-nitrocatechol 5-monooxygenase;
dntB (gene name);
4-methyl-5-nitrocatechol oxygenase;
MNC monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
4-methyl-5-nitrocatechol,NAD(P)H:oxygen 5-oxidoreductase (5-hydroxylating, nitrite-forming)
Reaction(IUBMB)
4-methyl-5-nitrocatechol + NAD(P)H + H+ + O2 = 2-hydroxy-5-methylquinone + nitrite + NAD(P)+ + H2O [RN:R09234 R11150]
Reaction(KEGG)
Substrate
4-methyl-5-nitrocatechol [CPD:C18315];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2-hydroxy-5-methylquinone [CPD:C18316];
nitrite [CPD:C00088];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Contains FAD. The enzyme, isolated from the bacterium Burkholderia sp. DNT, can use both NADH and NADPH, but prefers NADPH. It has a narrow substrate range, but can also act on 4-nitrocatechol.
History
EC 1.14.13.210 created 2016
Pathway
ec00633  Nitrotoluene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K21683  4-methyl-5-nitrocatechol 5-monooxygenase
Reference
1  [PMID:8830701]
  Authors
Haigler BE, Suen WC, Spain JC.
  Title
Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT.
  Journal
J Bacteriol 178:6019-24 (1996)
DOI:10.1128/JB.178.20.6019-6024.1996
  Sequence
Reference
2  [PMID:16751499]
  Authors
Leungsakul T, Johnson GR, Wood TK.
  Title
Protein engineering of the 4-methyl-5-nitrocatechol monooxygenase from Burkholderia sp. strain DNT for enhanced degradation of nitroaromatics.
  Journal
Appl Environ Microbiol 72:3933-9 (2006)
DOI:10.1128/AEM.02966-05
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.210
IUBMB Enzyme Nomenclature: 1.14.13.210
ExPASy - ENZYME nomenclature database: 1.14.13.210
BRENDA, the Enzyme Database: 1.14.13.210

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