Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

4-methyl-5-nitrocatechol,NAD(P)H:oxygen 5-oxidoreductase (5-hydroxylating, nitrite-forming)

4-methyl-5-nitrocatechol + NAD(P)H + H+ + O2 = 2-hydroxy-5-methylquinone + nitrite + NAD(P)+ + H2O [RN:R09234 R11150]

R09234 R11150
Reaction

4-methyl-5-nitrocatechol [CPD:C18315];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Contains FAD. The enzyme, isolated from the bacterium Burkholderia sp. DNT, can use both NADH and NADPH, but prefers NADPH. It has a narrow substrate range, but can also act on 4-nitrocatechol.

EC 1.14.13.210 created 2016

1  [PMID:8830701]

Haigler BE, Suen WC, Spain JC

Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT.

J Bacteriol 178:6019-24 (1996)
DOI:10.1128/JB.178.20.6019-6024.1996

[ag:AAC44479]

2  [PMID:16751499]

Leungsakul T, Johnson GR, Wood TK

Protein engineering of the 4-methyl-5-nitrocatechol monooxygenase from Burkholderia sp. strain DNT for enhanced degradation of nitroaromatics.

Appl Environ Microbiol 72:3933-9 (2006)
DOI:10.1128/AEM.02966-05

[ag:ABC00744]