| Entry |
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| Name |
4-methyl-5-nitrocatechol 5-monooxygenase;
dntB (gene name);
4-methyl-5-nitrocatechol oxygenase;
MNC monooxygenase
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
4-methyl-5-nitrocatechol,NAD(P)H:oxygen 5-oxidoreductase (5-hydroxylating, nitrite-forming)
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| Reaction(IUBMB) |
4-methyl-5-nitrocatechol + NAD(P)H + H+ + O2 = 2-hydroxy-5-methylquinone + nitrite + NAD(P)+ + H2O [RN: R09234 R11150]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Contains FAD. The enzyme, isolated from the bacterium Burkholderia sp. DNT, can use both NADH and NADPH, but prefers NADPH. It has a narrow substrate range, but can also act on 4-nitrocatechol.
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| History |
EC 1.14.13.210 created 2016
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| Pathway |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
| K21683 | 4-methyl-5-nitrocatechol 5-monooxygenase |
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| Reference |
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| Authors |
Haigler BE, Suen WC, Spain JC. |
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| Title |
Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Leungsakul T, Johnson GR, Wood TK. |
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| Title |
Protein engineering of the 4-methyl-5-nitrocatechol monooxygenase from Burkholderia sp. strain DNT for enhanced degradation of nitroaromatics. |
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| Journal |
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| Sequence |
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| Other DBs |
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