Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

rifampicin:NAD(P)H:oxygen oxidoreductase (2'-N-hydroxyrifampicin-forming)

rifampicin + NAD(P)H + O2 = 2'-N-hydroxyrifampicin + NAD(P)+ + H2O [RN:R11151 R11152]

R11151 R11152
Reaction

rifampicin [CPD:C06688];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007]

The enzyme has been found in the Corynebacteria Rhodococcus equi and Nocardia farcinica. It confers increased resistance to the antibiotic rifampicin by initiating its degradation.

EC 1.14.13.211 created 2016

1  [PMID:8980786]

Andersen SJ, Quan S, Gowan B, Dabbs ER

Monooxygenase-like sequence of a Rhodococcus equi gene conferring increased resistance to rifampin by inactivating this antibiotic.

Antimicrob Agents Chemother 41:218-21 (1997)
DOI:10.1128/AAC.41.1.218

[ag:AAB41059]

2  [PMID:19942945]

Hoshino Y, Fujii S, Shinonaga H, Arai K, Saito F, Fukai T, Satoh H, Miyazaki Y, Ishikawa J

Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance.

J Antibiot (Tokyo) 63:23-8 (2010)
DOI:10.1038/ja.2009.116