KEGG   ENZYME: 1.14.13.211
Entry
EC 1.14.13.211              Enzyme                                 

Name
rifampicin monooxygenase;
RIF-O
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
rifampicin:NAD(P)H:oxygen oxidoreductase (2'-N-hydroxyrifampicin-forming)
Reaction(IUBMB)
rifampicin + NAD(P)H + O2 = 2'-N-hydroxyrifampicin + NAD(P)+ + H2O [RN:R11151 R11152]
Reaction(KEGG)
R11151 R11152
Substrate
rifampicin [CPD:C06688];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007]
Product
2'-N-hydroxyrifampicin [CPD:C21143];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme has been found in the Corynebacteria Rhodococcus equi and Nocardia farcinica. It confers increased resistance to the antibiotic rifampicin by initiating its degradation.
History
EC 1.14.13.211 created 2016
Orthology
K21267  rifampicin monooxygenase
Genes
LMB: C9I47_1848
RHJ: HZY79_00590
MMC: Mmcs_2661
MKM: Mkms_2706
MJL: Mjls_2691
MYV: G155_10900
MSM: MSMEG_2252
MSG: MSMEI_2197
MSB: LJ00_11210
MSN: LI99_11210
MTHN: 4412656_04597(pcpB_2)
ASD: AS9A_2324
NFA: NFA_35380(rox)
NFR: ERS450000_00511(pcpB_1)
NOD: FOH10_14330(rox)
NAD: NCTC11293_02478(pcpB_3) NCTC11293_04330(pcpB_5)
RER: RER_20250
REY: O5Y_09730
ROP: ROP_37320
REQ: REQ_41440
RQI: C1M55_10225(iri)
RCR: NCTC10994_00474(pcpB)
GOM: D7316_01072(otcC)
SCO: SCO7626(SC2H2.24c)
SALB: XNR_3358
SBH: SBI_09593
SHY: SHJG_0647
SVE: SVEN_0481
SFI: SFUL_1083
SLD: T261_4795
STRM: M444_16460
SRW: TUE45_01151(pcpB_1)
STRD: NI25_36215
SLX: SLAV_11085(pcpB4)
SFK: KY5_0184
SGE: DWG14_01874(otcC_2) DWG14_06120(otcC_4)
SRJ: SRO_6922
SKY: D0C37_14590(rox)
SGD: ELQ87_04590(rox)
SCYA: EJ357_38800(rox)
KSK: KSE_07580
STRI: C7M71_013320(rox)
ART: Arth_1940
NDK: I601_2824(pcpB)
KFL: Kfla_1528
TFU: Tfu_2841
NDA: Ndas_2748
SRO: Sros_4669
NOW: GBF35_25415(rox)
SEN: SACE_4643
SACE: GIY23_21205(rox)
SACG: FDZ84_31035(rox)
SACC: EYD13_11195(pcpB2)
AMD: AMED_1629
AMN: RAM_08270
AMM: AMES_1617
AMZ: B737_1618
AOI: AORI_3930(mhpA)
AMYY: YIM_09330(pcpB1) YIM_16465(pcpB3)
AORI: SD37_20160
PDX: Psed_0253
PSEE: FRP1_14780
PAUT: Pdca_02570
AMI: Amir_2013
SSYI: EKG83_16990(rox)
KAL: KALB_4274
ACTI: UA75_15955
ACAD: UA74_15395
ALO: CRK57149
MIL: ML5_0967
MTEM: GCE86_10990(rox)
MCAB: HXZ27_08900(rox)
ASE: ACPL_5566
ACTN: L083_3384
ACTS: ACWT_5436
SNA: Snas_3217
CWO: Cwoe_4431
 » show all
Reference
1  [PMID:8980786]
  Authors
Andersen SJ, Quan S, Gowan B, Dabbs ER
  Title
Monooxygenase-like sequence of a Rhodococcus equi gene conferring increased resistance to rifampin by inactivating this antibiotic.
  Journal
Antimicrob Agents Chemother 41:218-21 (1997)
  Sequence
Reference
2  [PMID:19942945]
  Authors
Hoshino Y, Fujii S, Shinonaga H, Arai K, Saito F, Fukai T, Satoh H, Miyazaki Y, Ishikawa J
  Title
Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance.
  Journal
J Antibiot (Tokyo) 63:23-8 (2010)
DOI:10.1038/ja.2009.116
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.211
IUBMB Enzyme Nomenclature: 1.14.13.211
ExPASy - ENZYME nomenclature database: 1.14.13.211
BRENDA, the Enzyme Database: 1.14.13.211

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