KEGG   ENZYME: 1.14.13.211Help
Entry
EC 1.14.13.211              Enzyme                                 

Name
rifampicin monooxygenase;
RIF-O
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
rifampicin:NAD(P)H:oxygen oxidoreductase (2'-N-hydroxyrifampicin-forming)
Reaction(IUBMB)
rifampicin + NAD(P)H + O2 = 2'-N-hydroxyrifampicin + NAD(P)+ + H2O [RN:R11151 R11152]
Reaction(KEGG)
Substrate
rifampicin [CPD:C06688];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007]
Product
2'-N-hydroxyrifampicin [CPD:C21143];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme has been found in the Corynebacteria Rhodococcus equi and Nocardia farcinica. It confers increased resistance to the antibiotic rifampicin by initiating its degradation.
History
EC 1.14.13.211 created 2016
Orthology
K21267  rifampicin monooxygenase
Genes
RER: RER_20250
REY: O5Y_09730
REB: XU06_09965
Taxonomy
Reference
1  [PMID:8980786]
  Authors
Andersen SJ, Quan S, Gowan B, Dabbs ER
  Title
Monooxygenase-like sequence of a Rhodococcus equi gene conferring increased resistance to rifampin by inactivating this antibiotic.
  Journal
Antimicrob Agents Chemother 41:218-21 (1997)
  Sequence
Reference
2  [PMID:19942945]
  Authors
Hoshino Y, Fujii S, Shinonaga H, Arai K, Saito F, Fukai T, Satoh H, Miyazaki Y, Ishikawa J
  Title
Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance.
  Journal
J Antibiot (Tokyo) 63:23-8 (2010)
DOI:10.1038/ja.2009.116
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.211
IUBMB Enzyme Nomenclature: 1.14.13.211
ExPASy - ENZYME nomenclature database: 1.14.13.211
BRENDA, the Enzyme Database: 1.14.13.211

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