Entry
Name
rifampicin monooxygenase;
RIF-O;
ROX;
RIFMO;
rifampicin:NAD(P)H:oxygen oxidoreductase (2'-N-hydroxyrifampicin-forming) (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
rifampicin:NAD(P)H:oxygen oxidoreductase (2-hydroxy-2,27-secorifampicin-forming; ring-cleaving)
Reaction(IUBMB)
rifampicin + NAD(P)H + O2 = 2-hydroxy-2,27-secorifampicin + NAD(P)+ + H2O [RN:
R11151 R11152 ]
Reaction(KEGG)
Substrate
Product
Comment
The enzyme has been found in a variety of environmental bacteria, notably Rhodococcus, Nocardia, and Streptomyces. It hydroxylates C-2 of rifampicin leading to its macro-ring cleaving.
History
EC 1.14.13.211 created 2016, modified 2022
Orthology
K21267 rifampicin monooxygenase
Genes
MTHN : 4412656_04597(pcpB_2)
NFR : ERS450000_00511(pcpB_1)
NOZ : DMB37_35700 DMB37_35980(rox)
NAH : F5544_22005(rox) F5544_22150(rox) F5544_28800
NAD : NCTC11293_02478(pcpB_3) NCTC11293_04330(pcpB_5)
RCR : NCTC10994_00474(pcpB)
RKO : JWS14_00845(rox) JWS14_21170(rox)
RPSK : JWS13_23710(rox) JWS13_45025(rox)
SCB : SCAB_86061 SCAB_87811
SVT : SVTN_01150 SVTN_31975
SPRI : SPRI_0130 SPRI_7223
SCX : AS200_01595 AS200_42295 AS200_44240
SGM : GCM10017557_73960 GCM10017557_80250 GCM10017557_82510
SALJ : SMD11_6238 SMD11_6656
SGE : DWG14_01874(otcC_2) DWG14_06120(otcC_4)
SKA : CP970_36715 CP970_42000(rox)
SNF : JYK04_00573(otcC_3) JYK04_00810(otcC_4)
SHUN : DWB77_00315(otcC_1) DWB77_07437(otcC_8)
SFEU : IM697_28010(rox) IM697_28330(rox)
SLF : JEQ17_20780(rox) JEQ17_46830(rox)
YIA : LO772_01570(rox) LO772_34465
KQI : F1D05_12920(rox) F1D05_27825(rox) F1D05_30245
NCX : Nocox_22045(pcpB2) Nocox_33465(pcpB6)
NGN : LCN96_27720 LCN96_32520(rox)
AMYC : CU254_15995 CU254_19080
AMYB : BKN51_12065 BKN51_12125
AAB : A4R43_00220 A4R43_03555
AMYY : YIM_09330(pcpB1) YIM_16465(pcpB3)
SESP : BN6_42360 BN6_44160
LED : BBK82_36645 BBK82_39920
ACTU : Actkin_03072(pcpB_3) Actkin_04232(pcpB_4)
MICH : FJK98_20740 FJK98_25315(rox)
ATL : Athai_28500 Athai_49560 Athai_60150
DVC : Dvina_10845 Dvina_22025(rox)
DAUR : Daura_18630(rox) Daura_28680 Daura_31715(rox)
DMAT : Dmats_10440 Dmats_17640(rox)
» show all
Taxonomy
Reference
Authors
Andersen SJ, Quan S, Gowan B, Dabbs ER
Title
Monooxygenase-like sequence of a Rhodococcus equi gene conferring increased resistance to rifampin by inactivating this antibiotic.
Journal
Sequence
Reference
Authors
Hoshino Y, Fujii S, Shinonaga H, Arai K, Saito F, Fukai T, Satoh H, Miyazaki Y, Ishikawa J
Title
Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance.
Journal
Sequence
Reference
Authors
Koteva K, Cox G, Kelso JK, Surette MD, Zubyk HL, Ejim L, Stogios P, Savchenko A, Sorensen D, Wright GD
Title
Rox, a Rifamycin Resistance Enzyme with an Unprecedented Mechanism of Action.
Journal
Sequence
Reference
Authors
Liu LK, Dai Y, Abdelwahab H, Sobrado P, Tanner JJ.
Title
Structural Evidence for Rifampicin Monooxygenase Inactivating Rifampicin by Cleaving Its Ansa-Bridge.
Journal
Sequence
Other DBs