KEGG   ENZYME: 1.14.13.212Help
Entry
EC 1.14.13.212              Enzyme                                 

Name
1,3,7-trimethyluric acid 5-monooxygenase;
tmuM (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
1,3,7-trimethylurate,NADH:oxygen oxidoreductase (1,3,7-trimethyl-5-hydroxyisourate forming)
Reaction(IUBMB)
1,3,7-trimethylurate + NADH + H+ + O2 = 1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O [RN:R11153]
Reaction(KEGG)
Substrate
1,3,7-trimethylurate [CPD:C16361];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
1,3,7-trimethyl-5-hydroxyisourate [CPD:C21144];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC 1.14.13.113, FAD-dependent urate hydroxylase.
History
EC 1.14.13.212 created 2016
Pathway
ec00232  Caffeine metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K00492  1,3,7-trimethyluric acid 5-monooxygenase
Reference
1  [PMID:22609920]
  Authors
Mohanty SK, Yu CL, Das S, Louie TM, Gakhar L, Subramanian M
  Title
Delineation of the caffeine C-8 oxidation pathway in Pseudomonas sp. strain CBB1  via characterization of a new trimethyluric acid monooxygenase and genes involved in trimethyluric acid metabolism.
  Journal
J Bacteriol 194:3872-82 (2012)
DOI:10.1128/JB.00597-12
  Sequence
Reference
2  [PMID:25678373]
  Authors
Summers RM, Mohanty SK, Gopishetty S, Subramanian M
  Title
Genetic characterization of caffeine degradation by bacteria and its potential applications.
  Journal
Microb Biotechnol 8:369-78 (2015)
DOI:10.1111/1751-7915.12262
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.212
IUBMB Enzyme Nomenclature: 1.14.13.212
ExPASy - ENZYME nomenclature database: 1.14.13.212
BRENDA, the Enzyme Database: 1.14.13.212

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