KEGG   ENZYME: 1.14.13.215Help
Entry
EC 1.14.13.215              Enzyme                                 

Name
protoasukamycin 4-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
protoasukamycin,NADH:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
protoasukamycin + NADH + H+ + O2 = 4-hydroxyprotoasukamycin + NAD+ + H2O [RN:R11155]
Reaction(KEGG)
Substrate
protoasukamycin [CPD:C21145];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
4-hydroxyprotoasukamycin [CPD:C21146];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Streptomyces nodosus subsp. asukaensis, is involved in the biosynthesis of the antibiotic asukamycin. Requires a flavin cofactor, with no preference among FMN, FAD or riboflavin. When flavin concentration is low, activity is enhanced by the presence of the NADH-dependent flavin-reductase AsuE2.
History
EC 1.14.13.215 created 2016
Reference
1  [PMID:23890006]
  Authors
Rui Z, Sandy M, Jung B, Zhang W
  Title
Tandem enzymatic oxygenations in biosynthesis of epoxyquinone pharmacophore of manumycin-type metabolites.
  Journal
Chem Biol 20:879-87 (2013)
DOI:10.1016/j.chembiol.2013.05.006
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.215
IUBMB Enzyme Nomenclature: 1.14.13.215
ExPASy - ENZYME nomenclature database: 1.14.13.215
BRENDA, the Enzyme Database: 1.14.13.215

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