| Entry |
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| Name |
protoasukamycin 4-monooxygenase
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
protoasukamycin,NADH:oxygen oxidoreductase (4-hydroxylating)
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| Reaction(IUBMB) |
protoasukamycin + NADH + H+ + O2 = 4-hydroxyprotoasukamycin + NAD+ + H2O [RN: R11155]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
The enzyme, characterized from the bacterium Streptomyces nodosus subsp. asukaensis, is involved in the biosynthesis of the antibiotic asukamycin. Requires a flavin cofactor, with no preference among FMN, FAD or riboflavin. When flavin concentration is low, activity is enhanced by the presence of the NADH-dependent flavin-reductase AsuE2.
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| History |
EC 1.14.13.215 created 2016
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| Reference |
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| Authors |
Rui Z, Sandy M, Jung B, Zhang W |
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| Title |
Tandem enzymatic oxygenations in biosynthesis of epoxyquinone pharmacophore of manumycin-type metabolites. |
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| Journal |
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| Other DBs |
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