KEGG   ENZYME: 1.14.13.217Help
Entry
EC 1.14.13.217              Enzyme                                 

Name
protodeoxyviolaceinate monooxygenase;
vioD (gene name);
protoviolaceinate synthase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
protodeoxyviolaceinate,NAD(P)H:O2 oxidoreductase
Reaction(IUBMB)
protodeoxyviolaceinate + NAD(P)H + O2 = protoviolaceinate + NAD(P)+ + H2O [RN:R11134 R11158]
Reaction(KEGG)
Substrate
protodeoxyviolaceinate [CPD:C21131];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007]
Product
protoviolaceinate [CPD:C21134];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Chromobacterium violaceum, participates in the biosynthesis of the violet pigment violacein. The product, protoviolaceinate, can be acted upon by EC 1.14.13.224, violacein synthase, leading to violacein production. However, it is very labile, and in the presence of oxygen can undergo non-enzymic autooxidation to the shunt product proviolacein.
History
EC 1.14.13.217 created 2016, modified 2016
Pathway
ec00404  Staurosporine biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K20089  protodeoxyviolaceinate monooxygenase
Genes
CVI: CV_3271(vioD)
CVC: BKX93_07100
CHRI: DK842_01225
JAG: GJA_1083(vioD)
JAZ: YQ44_10555
JAL: BZG29_16960
MNR: ACZ75_19205
MASS: CR152_21620
RIV: Riv7116_3967
Taxonomy
Reference
1  [PMID:17176066]
  Authors
Balibar CJ, Walsh CT
  Title
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.
  Journal
Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z
Reference
2  [PMID:17925955]
  Authors
Shinoda K, Hasegawa T, Sato H, Shinozaki M, Kuramoto H, Takamiya Y, Sato T, Nikaidou N, Watanabe T, Hoshino T.
  Title
Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function.
  Journal
Chem Commun (Camb) 4140-2 (2007)
DOI:10.1039/b705358d
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.217
IUBMB Enzyme Nomenclature: 1.14.13.217
ExPASy - ENZYME nomenclature database: 1.14.13.217
BRENDA, the Enzyme Database: 1.14.13.217

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