KEGG   ENZYME: 1.14.13.218Help
Entry
EC 1.14.13.218              Enzyme                                 

Name
5-methylphenazine-1-carboxylate 1-monooxygenase;
phzS (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
5-methylphenazine-1-carboxylate,NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)
Reaction(IUBMB)
5-methylphenazine-1-carboxylate + NADH + O2 = pyocyanin + NAD+ + CO2 + H2O [RN:R11346]
Reaction(KEGG)
R11346;
(other) R11578
Show
Substrate
5-methylphenazine-1-carboxylate [CPD:C21295];
NADH [CPD:C00004];
O2 [CPD:C00007]
Product
pyocyanin [CPD:C01748];
NAD+ [CPD:C00003];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Pseudomonas aeruginosa, is involved in the biosynthesis of pyocyanin, a toxin produced and secreted by the organism. It can also act on phenazine-1-carboxylate, converting it into phenazin-1-ol.
History
EC 1.14.13.218 created 2016
Pathway
ec00405  Phenazine biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K20940  5-methylphenazine-1-carboxylate 1-monooxygenase
Genes
ROR: RORB6_06595
RPLN: B1209_13685
RAO: DSD31_13210
EBF: D782_1281
PAO: Pat9b_1855
LAB: LA76x_3342(phzS)
LAQ: GLA29479_2375(phzS)
PAE: PA4217(phzS)
PAEV: N297_4349
PAEI: N296_4349
PAU: PA14_09400(phzS)
PAG: PLES_07101(phzS)
PAF: PAM18_0720(phzS)
PNC: NCGM2_5462(phzS)
PAEB: NCGM1900_0742(phzS)
PAEP: PA1S_03735
PAEM: U769_03765
PAEL: T223_03665
PAEU: BN889_04688(mhbM)
PAEG: AI22_00175
PAEC: M802_4347
PAEO: M801_4215
GAI: IMCC3135_15925(xlnD)
RPI: Rpic_0514
REH: H16_A0578(h16_A0578)
CGD: CR3_2101(ubiH)
BMA: BMAA0391
BMAL: DM55_3356
BMAE: DM78_4291
BMAQ: DM76_4054
BMAI: DM57_4923
BMAF: DM51_4113
BMAZ: BM44_3316
BMAB: BM45_4154
BPS: BPSS2111
BPSE: BDL_5573
BPSM: BBQ_4004(phzS)
BPSU: BBN_5593(phzS)
BPSD: BBX_4740(phzS)
BPK: BBK_4877(phzS)
BPSH: DR55_5621
BPSA: BBU_3886(phzS)
BPSO: X996_5401
BUT: X994_4948
BCEN: DM39_5111
PPUL: RO07_14100
PSPU: NA29_23010
PAPI: SG18_13925
BPT: Bpet0515
PUT: PT7_0833
POL: Bpro_2199
AAV: Aave_0794
AAA: Acav_0721
ACRA: BSY15_1202
DAC: Daci_4386
RTA: Rta_23930
LIM: L103DPR2_01284(xlnD_2)
HYB: Q5W_04140
MES: Meso_2927
OAN: Oant_3996
OAH: DR92_4259
BJA: bll2502
BRA: BRADO0592
BBT: BBta_2684
RPA: RPA3588
RPB: RPB_1945
RPT: Rpal_4108
VGO: GJW-30_1_00854(xlnD_1)
MET: M446_3637
MNO: Mnod_1206
MAQU: Maq22A_c15030(ubiH)
RBM: TEF_16205
SIL: SPO0105
PGD: Gal_00046
LAQU: R2C4_19230
SPSE: SULPSESMR1_00290(hpxO)
RID: RIdsm_02011(xlnD)
NAR: Saro_2238
SWI: Swit_3061
SPHD: HY78_10270
ACR: Acry_0321
TMO: TMO_3126(mhbM)
BKW: BkAM31D_08555(xlnD_1)
MHAS: MHAS_01663(nicC)
MAB: MAB_2237c
MABB: MASS_2167
MCHE: BB28_11255
MSAL: DSM43276_01950(xlnD_2)
SBH: SBI_01059
SVE: SVEN_5103
STRD: NI25_24410
SMAL: SMALA_0139
SFK: KY5_2781c
LMOI: VV02_01275
IDO: I598_2461(hpxO_3)
CFL: Cfla_0298
NDA: Ndas_5023
SRO: Sros_6172
PDX: Psed_3194
PAUT: Pdca_23520
CAI: Caci_4355
SLI: Slin_0336
 » show all
Taxonomy
Reference
1  [PMID:11591691]
  Authors
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
  Title
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
  Journal
J Bacteriol 183:6454-65 (2001)
DOI:10.1128/JB.183.21.6454-6465.2001
  Sequence
[pae:PA4217]
Reference
2  [PMID:17253782]
  Authors
Parsons JF, Greenhagen BT, Shi K, Calabrese K, Robinson H, Ladner JE
  Title
Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.
  Journal
Biochemistry 46:1821-8 (2007)
DOI:10.1021/bi6024403
Reference
3  [PMID:18416536]
  Authors
Greenhagen BT, Shi K, Robinson H, Gamage S, Bera AK, Ladner JE, Parsons JF
  Title
Crystal structure of the pyocyanin biosynthetic protein PhzS.
  Journal
Biochemistry 47:5281-9 (2008)
DOI:10.1021/bi702480t
  Sequence
[pae:PA4217]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.218
IUBMB Enzyme Nomenclature: 1.14.13.218
ExPASy - ENZYME nomenclature database: 1.14.13.218
BRENDA, the Enzyme Database: 1.14.13.218

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