KEGG   ENZYME: 1.14.13.220Help
Entry
EC 1.14.13.220              Enzyme                                 

Name
resorcinol 4-hydroxylase (NADH);
tsdB (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
resorcinol,NADH:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
resorcinol + NADH + H+ + O2 = hydroxyquinol + NAD+ + H2O [RN:R11347]
Reaction(KEGG)
Substrate
resorcinol [CPD:C01751];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
hydroxyquinol [CPD:C02814];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Rhodococcus jostii RHA1, is a single-component hydroxylase. The enzyme has no activity with NADPH. cf. EC 1.14.13.219, resorcinol 4-hydroxylase (NADPH), and EC 1.14.14.27, resorcinol 4-hydroxylase (FADH2).
History
EC 1.14.13.220 created 2016
Pathway
ec00362  Benzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K20944  resorcinol 4-hydroxylase (NADH)
Genes
RHA: RHA1_ro01860
Taxonomy
Reference
1  [PMID:26319878]
  Authors
Kasai D, Araki N, Motoi K, Yoshikawa S, Iino T, Imai S, Masai E, Fukuda M
  Title
gamma-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1.
  Journal
Appl Environ Microbiol 81:7656-65 (2015)
DOI:10.1128/AEM.02422-15
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.220
IUBMB Enzyme Nomenclature: 1.14.13.220
ExPASy - ENZYME nomenclature database: 1.14.13.220
BRENDA, the Enzyme Database: 1.14.13.220

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