KEGG   ENZYME: 1.14.13.223
Entry
EC 1.14.13.223              Enzyme                                 
Name
3-hydroxy-4-methylanthranilyl-[aryl-carrier protein] 5-monooxygenase;
sibG (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
3-hydroxy-4-methylanthranilyl-[aryl-carrier protein],NADH:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
3-hydroxy-4-methylanthranilyl-[aryl-carrier protein] + NADH + H+ + O2 = 3,5-dihydroxy-4-methylanthranilyl-[aryl-carrier protein] + NAD+ + H2O [RN:R11424]
Reaction(KEGG)
R11424
Substrate
3-hydroxy-4-methylanthranilyl-[aryl-carrier protein] [CPD:C21375];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3,5-dihydroxy-4-methylanthranilyl-[aryl-carrier protein] [CPD:C21376];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). The enzyme, characterized from the bacterium Streptosporangium sibiricum, is involved in the biosynthesis of the antitumor antibiotic sibiromycin. The enzyme is not active with free 3-hydroxy-4-methylanthranilate.
History
EC 1.14.13.223 created 2016
Orthology
K21280  3-hydroxy-4-methylanthranilyl-[aryl-carrier protein] 5-monooxygenase
Reference
1  [PMID:21612226]
  Authors
Giessen TW, Kraas FI, Marahiel MA
  Title
A four-enzyme pathway for 3,5-dihydroxy-4-methylanthranilic acid formation and incorporation into the antitumor antibiotic sibiromycin.
  Journal
Biochemistry 50:5680-92 (2011)
DOI:10.1021/bi2006114
  Sequence
[ag:ACN39730]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.223
IUBMB Enzyme Nomenclature: 1.14.13.223
ExPASy - ENZYME nomenclature database: 1.14.13.223
BRENDA, the Enzyme Database: 1.14.13.223

  All links  
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (3)   
   InterPro (3)   
All databases (16)   

Download RDF
DBGET integrated database retrieval system