KEGG   ENZYME: 1.14.13.223Help
Entry
EC 1.14.13.223              Enzyme                                 

Name
3-hydroxy-4-methylanthranilyl-[aryl-carrier protein] 5-monooxygenase;
sibG (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-hydroxy-4-methylanthranilyl-[aryl-carrier protein],NADH:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
3-hydroxy-4-methylanthranilyl-[aryl-carrier protein] + NADH + H+ + O2 = 3,5-dihydroxy-4-methylanthranilyl-[aryl-carrier protein] + NAD+ + H2O [RN:R11424]
Reaction(KEGG)
Substrate
3-hydroxy-4-methylanthranilyl-[aryl-carrier protein] [CPD:C21375];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3,5-dihydroxy-4-methylanthranilyl-[aryl-carrier protein] [CPD:C21376];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). The enzyme, characterized from the bacterium Streptosporangium sibiricum, is involved in the biosynthesis of the antitumor antibiotic sibiromycin. The enzyme is not active with free 3-hydroxy-4-methylanthranilate.
History
EC 1.14.13.223 created 2016
Orthology
K21280  3-hydroxy-4-methylanthranilyl-[aryl-carrier protein] 5-monooxygenase
Reference
1  [PMID:21612226]
  Authors
Giessen TW, Kraas FI, Marahiel MA
  Title
A four-enzyme pathway for 3,5-dihydroxy-4-methylanthranilic acid formation and incorporation into the antitumor antibiotic sibiromycin.
  Journal
Biochemistry 50:5680-92 (2011)
DOI:10.1021/bi2006114
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.223
IUBMB Enzyme Nomenclature: 1.14.13.223
ExPASy - ENZYME nomenclature database: 1.14.13.223
BRENDA, the Enzyme Database: 1.14.13.223

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