KEGG   ENZYME: 1.14.13.224
Entry
EC 1.14.13.224              Enzyme                                 

Name
violacein synthase;
proviolaceinate monooxygenase;
vioC (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
protoviolaceinate,NAD(P)H:O2 oxidoreductase
Reaction(IUBMB)
(1) protoviolaceinate + NAD(P)H + O2 = violaceinate + NAD(P)+ + H2O [RN:R11135 R11373];
(2) protodeoxyviolaceinate + NAD(P)H + O2 = deoxyviolaceinate + NAD(P)+ + H2O [RN:R11132 R11374]
Reaction(KEGG)
Substrate
protoviolaceinate [CPD:C21134];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007];
protodeoxyviolaceinate [CPD:C21131]
Product
violaceinate [CPD:C21135];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
deoxyviolaceinate [CPD:C21132]
Comment
The enzyme, characterized from the bacterium Chromobacterium violaceum, participates in the biosynthesis of the violet pigment violacein. The products, violaceinate and deoxyviolaceinate, undergo non-enzymic autooxidation into violacein and deoxyviolacein, respectively.
History
EC 1.14.13.224 created 2016
Pathway
ec00404  Staurosporine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20090  violacein synthase
Genes
CVI: CV_3272(vioC)
CVC: BKX93_07105
CHRI: DK842_01230
CHRB: DK843_06510
CHRM: FYK34_10585
IFL: C1H71_10080
JAG: GJA_1082(vioC)
JAZ: YQ44_10560
JAL: BZG29_16955
JLV: G3257_17685(vioC)
 » show all
Reference
1  [PMID:17176066]
  Authors
Balibar CJ, Walsh CT
  Title
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.
  Journal
Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z
Reference
2  [PMID:17925955]
  Authors
Shinoda K, Hasegawa T, Sato H, Shinozaki M, Kuramoto H, Takamiya Y, Sato T, Nikaidou N, Watanabe T, Hoshino T.
  Title
Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function.
  Journal
Chem Commun (Camb) 4140-2 (2007)
DOI:10.1039/b705358d
  Sequence
[cvi:CV_3272]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.224
IUBMB Enzyme Nomenclature: 1.14.13.224
ExPASy - ENZYME nomenclature database: 1.14.13.224
BRENDA, the Enzyme Database: 1.14.13.224

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