EC              Enzyme                                 

F-actin monooxygenase;
MICAL (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
[F-actin]-L-methionine,NADPH:O2 S-oxidoreductase
[F-actin]-L-methionine + NADPH + O2 + H+ = [F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O [RN:R11425]
[F-actin]-L-methionine [CPD:C21377];
NADPH [CPD:C00005];
O2 [CPD:C00007];
H+ [CPD:C00080]
[F-actin]-L-methionine-(R)-S-oxide [CPD:C21378];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
The enzyme, characterized from the fruit fly Drosophila melanogaster, is a multi-domain oxidoreductase that acts as an F-actin disassembly factor. The enzyme selectively reduces two L-Met residues of F-actin, causing fragmentation of the filaments and preventing repolymerization [1]. Free methionine is not a substrate [2]. The reaction is stereospecific and generates the (R)-sulfoxide [3]. In the absence of substrate, the enzyme can act as an NAD(P)H oxidase (EC [4,5].
EC created 2016
K19947  F-actin monooxygenase
HSA: 57553(MICAL3) 64780(MICAL1) 9645(MICAL2)
PTR: 451031(MICAL2) 463145(MICAL1) 745678(MICAL3)
PPS: 100969548(MICAL1) 100987179(MICAL3) 100994154(MICAL2)
GGO: 101127991(MICAL1) 101139251(MICAL2) 101149053(MICAL3)
PON: 100173765(MICAL1) 100438047(MICAL2) 100444220(MICAL3)
NLE: 100581557(MICAL3) 100599627(MICAL2) 100604034(MICAL1)
MCC: 698737(MICAL1) 701439 710292(MICAL3)
MCF: 102124064(MICAL2) 102135771(MICAL3) 102139619(MICAL1)
CSAB: 103222878(MICAL3) 103240067(MICAL2) 103240597(MICAL1)
RRO: 104668570 104668827(MICAL1) 104676297(MICAL3)
RBB: 108533801(MICAL1) 108535533(MICAL2) 108541715(MICAL3)
CJC: 100394528(MICAL2) 100395442(MICAL1) 100400227(MICAL3)
SBQ: 101027900(MICAL2) 101040006(MICAL3) 101042616(MICAL1)
MMU: 171580(Mical1) 194401(Mical3) 320878(Mical2)
MCAL: 110295772(Mical3) 110299452(Mical2) 110303515(Mical1)
MPAH: 110314399(Mical3) 110318852(Mical2) 110326332(Mical1)
RNO: 294520(Mical1) 362427(Mical3) 365352(Mical2)
MUN: 110541039(Mical2) 110546057(Mical3) 110555442(Mical1)
CGE: 100752579(Mical1) 100754319(Mical3) 100763886(Mical2)
NGI: 103745899(Mical3) 103749557(Mical1) 103750410(Mical2)
HGL: 101701740(Mical1) 101709910(Mical3) 101712859(Mical2)
CCAN: 109684398 109684399 109688102(Mical2) 109694751(Mical1)
OCU: 100347412(MICAL1) 100353897(MICAL2) 100354238(MICAL3)
TUP: 102480907(MICAL3) 102486503(MICAL1) 102494378(MICAL2)
CFA: 476858(MICAL2) 481958(MICAL1) 486761(MICAL3)
VVP: 112917441(MICAL3) 112925126(MICAL1) 112927059(MICAL2)
AML: 100465187(MICAL3) 100465934(MICAL2) 100478759(MICAL1)
UMR: 103675754(MICAL3) 103677024(MICAL1) 103682359(MICAL2)
UAH: 113246261 113258066(MICAL3) 113264746(MICAL1)
ORO: 101366928(MICAL2) 101371709(MICAL3) 101380122(MICAL1)
FCA: 101081558(MICAL3) 101091046 101100722(MICAL1)
PTG: 102951530(MICAL3) 102962220(MICAL1) 102962641(MICAL2)
PPAD: 109263525(MICAL1) 109271148 109276281(MICAL3)
AJU: 106968456(MICAL3) 106968890(MICAL1) 106971840
BTA: 508306(MICAL1) 532244(MICAL3) 534041(MICAL2)
BOM: 102266509(MICAL2) 102273179(MICAL1) 102286656(MICAL3)
BIU: 109559784(MICAL3) 109563835(MICAL1) 109569548(MICAL2)
BBUB: 102391634(MICAL1) 102395599(MICAL3) 102401601
CHX: 102172084(MICAL2) 102178522(MICAL3) 102185000(MICAL1)
OAS: 101113940(MICAL1) 101118097(MICAL3) 101121880(MICAL2)
SSC: 100153346(MICAL1) 100157721(MICAL3) 100519075(MICAL2)
CFR: 102507940 102511399(MICAL1) 102522111(MICAL3)
CDK: 105086699 105097110(MICAL1) 105100736(MICAL3)
BACU: 103000884(MICAL1) 103012504(MICAL3) 103012861(MICAL2)
LVE: 103069359(MICAL2) 103087508(MICAL1) 103088697(MICAL3)
OOR: 101275370(MICAL2) 101275745(MICAL3) 101285142(MICAL1)
DLE: 111164423(MICAL1) 111165227(MICAL2) 111176586(MICAL3)
PCAD: 102977044(MICAL1) 102988858(MICAL2) 102991718(MICAL3)
ECB: 100054807(MICAL3) 100055978 100066627(MICAL1)
EPZ: 103551111(MICAL1) 103565092(MICAL3) 103566075
EAI: 106829758(MICAL1) 106833091(MICAL2) 106839442(MICAL3)
MYB: 102248958(MICAL2) 102251692(MICAL1) 102258499(MICAL3)
MYD: 102751644(MICAL3) 102759552(MICAL1) 102769793(MICAL2)
MNA: 107535318(MICAL2) 107537711(MICAL3) 107540706(MICAL1)
HAI: 109373294(MICAL3) 109388271 109391423(MICAL1)
DRO: 112297007(MICAL1) 112303258 112311619(MICAL3)
PALE: 102887125(MICAL3) 102889705(MICAL2) 102892071(MICAL1)
RAY: 107500353(MICAL3) 107505345(MICAL2) 107518492(MICAL1)
MJV: 108395279(MICAL2) 108397551(MICAL1) 108406778
LAV: 100661909(MICAL3) 100663700 100665365(MICAL1)
MDO: 100022040(MICAL3) 100029104(MICAL1) 100030142(MICAL2) 107649961
SHR: 100920737(MICAL1) 100922670(MICAL2) 100929940(MICAL3)
PCW: 110218729(MICAL3) 110218891(MICAL2) 110222901(MICAL1)
OAA: 100073551(MICAL2) 100085361(MICAL1) 100086054(MICAL3)
GGA: 418164(MICAL3) 423031(MICAL2) 429474(MICAL1)
CJO: 107314574(MICAL2) 107314988(MICAL3) 107324612(MICAL1)
NMEL: 110388263(MICAL1) 110395577(MICAL3) 110400782(MICAL2)
APLA: 101794484(MICAL2) 101803780(MICAL1) 101805047(MICAL3)
ACYG: 106030921(MICAL3) 106040898(MICAL2) 106046127
TGU: 100219168(MICAL3) 100227450(MICAL2) 100232670(MICAL1)
LSR: 110469104(MICAL2) 110473459(MICAL1) 110482582(MICAL3)
SCAN: 103820469(MICAL3) 103823343(MICAL1) 103824313(MICAL2)
GFR: 102032677(MICAL3) 102033849(MICAL2) 102043330(MICAL1)
FAB: 101811190(MICAL2) 101819614(MICAL3) 101820098(MICAL1)
PHI: 102107564(MICAL3) 102107919(MICAL2) 102108109(MICALCL) 102108714(MICAL1)
PMAJ: 107204329(MICAL3) 107205792(MICAL2) 107214787(MICAL1)
CCAE: 111928083(MICAL3) 111930849(MICAL2) 111939748(MICAL1)
CCW: 104692251(MICAL3) 104692546(MICAL1) 104692969
ETL: 114056527(MICAL2) 114065769(MICAL1) 114068977(MICAL3)
FPG: 101914505(MICAL3) 101922313(MICAL1) 101924713(MICAL2)
FCH: 102047357(MICAL3) 102052557(MICAL2) 102060220(MICAL1)
CLV: 102085132(MICAL2) 102093478(MICAL3) 102096460(MICAL1)
EGZ: 104123406(MICAL2) 104125757(MICAL1) 104134379(MICAL3)
NNI: 104009445(MICAL2) 104013230(MICAL1) 104020203(MICAL3)
ACUN: 113481325(MICAL2) 113489033(MICAL1) 113491284(MICAL3)
PADL: 103919887 103924360(MICAL3) 103925723(MICAL2) 103925796(MICALCL)
AAM: 106487711(MICAL1) 106488631(MICAL3) 106497117(MICAL2)
ASN: 102371980 102375438(MICAL3) 102377820(MICAL2)
AMJ: 102565781(MICAL1) 102574107(MICALCL) 106737063(MICAL3)
PSS: 102443943(MICAL3) 102447311 102462749(MICAL2)
CMY: 102932362(MICAL2) 102934250(MICALCL) 102935764(MICAL1) 102942117(MICAL3)
CPIC: 101936236(MICAL1) 101936780(MICAL3) 101946730(MICAL2)
ACS: 100552986(mical1) 100554427(mical3) 100564862(mical2)
PVT: 110076703(MICAL3) 110079674(MICAL1) 110080915(MICAL2)
PBI: 103048923 103058605(MICAL1) 103058722(MICAL2) 103064154
PMUR: 107289040(MICAL1) 107292277(MICAL3) 107293552
PMUA: 114597808(MICAL2) 114599198 114604933(MICAL3)
XTR: 100486205(mical3) 100487018(mical2)
NPR: 108786140(MICAL2) 108791576(MICAL3)
DRE: 402881(mical3a) 566850(mical3b) 568573(mical1) 569564(mical2b) 569607(mical2a)
TRU: 101067707(mical2) 101068062 101075455 101078087(mical3)
LCM: 102345089 102360081(MICAL3) 102366021(MICAL2)
BFO: 118406631
CIN: 100175527
SPU: 582210
APLC: 110989175
SKO: 100378899
DME: Dmel_CG33208(Mical)
DER: 6552049
DSE: 6607011
DSI: Dsimw501_GD20744(Dsim_GD20744)
DSR: 110188462
DPE: 6598648
DMN: 108155526
DWI: 6647152
DAZ: 108615232
DNV: 108658541
DHE: 111595671
MDE: 101887289
LCQ: 111690633
AAG: 5568484
AME: 413310
BIM: 100744389
BTER: 100650962
CCAL: 108628034
OBB: 114878658
SOC: 105204672
AEC: 105145217
ACEP: 105617147
PBAR: 105423576
VEM: 105565851
DQU: 106741595
CFO: 105257878
LHU: 105675006
PGC: 109854698
OBO: 105283811
PCF: 106785377
NVI: 100120752
CSOL: 105360342
MDL: 103579831
TCA: 100141546
NVL: 108557182
BMOR: 101742566(T15.180a)
BMAN: 114246423
PMAC: 106717230
PRAP: 111003396
HAW: 110382349
TNL: 113503945
API: 100168266
DNX: 107162729
RMD: 113550115
BTAB: 109029643
CLEC: 106674386
ZNE: 110827699
TUT: 107372243
DPTE: 113794869
CSCU: 111623901
PTEP: 107453849
BMY: Bm1_43575
CRG: 105346067
MYI: 110457951
OBI: 106870794
LAK: 106178150
SHX: MS3_03588
EGL: EGR_02335
ADF: 107332908
SMIN: v1.2.023094.t1(symbB.v1.2.023094.t1) v1.2.023094.t2(symbB.v1.2.023094.t2) v1.2.027574.t1(symbB.v1.2.027574.t1)
SPAR: SPRG_02957
 » show all
1  [PMID:20148037]
Hung RJ, Yazdani U, Yoon J, Wu H, Yang T, Gupta N, Huang Z, van Berkel WJ, Terman JR
Mical links semaphorins to F-actin disassembly.
Nature 463:823-7 (2010)
2  [PMID:22116028]
Hung RJ, Pak CW, Terman JR
Direct redox regulation of F-actin assembly and disassembly by Mical.
Science 334:1710-3 (2011)
3  [PMID:24212093]
Hung RJ, Spaeth CS, Yesilyurt HG, Terman JR
SelR reverses Mical-mediated oxidation of actin to regulate F-actin dynamics.
Nat Cell Biol 15:1445-54 (2013)
4  [PMID:21864500]
Zucchini D, Caprini G, Pasterkamp RJ, Tedeschi G, Vanoni MA
Kinetic and spectroscopic characterization of the putative monooxygenase domain of human MICAL-1.
Arch Biochem Biophys 515:1-13 (2011)
5  [PMID:26845023]
Vitali T, Maffioli E, Tedeschi G, Vanoni MA
Properties and catalytic activities of MICAL1, the flavoenzyme involved in cytoskeleton dynamics, and modulation by its CH, LIM and C-terminal domains.
Arch Biochem Biophys 593:24-37 (2016)
Other DBs
ExplorEnz - The Enzyme Database:
IUBMB Enzyme Nomenclature:
ExPASy - ENZYME nomenclature database:
BRENDA, the Enzyme Database:

DBGET integrated database retrieval system