KEGG   ENZYME: 1.14.13.225Help
Entry
EC 1.14.13.225              Enzyme                                 

Name
F-actin monooxygenase;
MICAL (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
[F-actin]-L-methionine,NADPH:O2 S-oxidoreductase
Reaction(IUBMB)
[F-actin]-L-methionine + NADPH + O2 + H+ = [F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O [RN:R11425]
Reaction(KEGG)
Substrate
[F-actin]-L-methionine [CPD:C21377];
NADPH [CPD:C00005];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
[F-actin]-L-methionine-(R)-S-oxide [CPD:C21378];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the fruit fly Drosophila melanogaster, is a multi-domain oxidoreductase that acts as an F-actin disassembly factor. The enzyme selectively reduces two L-Met residues of F-actin, causing fragmentation of the filaments and preventing repolymerization [1]. Free methionine is not a substrate [2]. The reaction is stereospecific and generates the (R)-sulfoxide [3]. In the absence of substrate, the enzyme can act as an NAD(P)H oxidase (EC 1.6.3.1) [4,5].
History
EC 1.14.13.225 created 2016
Orthology
K19947  F-actin monooxygenase
Genes
HSA: 57553(MICAL3) 64780(MICAL1) 9645(MICAL2)
PTR: 451031(MICAL2) 463145(MICAL1) 745678(MICAL3)
PPS: 100969548(MICAL1) 100987179(MICAL3) 100994154(MICAL2)
GGO: 101127991(MICAL1) 101139251(MICAL2) 101144122 101149053
PON: 100173765(MICAL1) 100438047(MICAL2) 100444220(MICAL3)
NLE: 100581557(MICAL3) 100599627(MICAL2) 100604034(MICAL1)
MCC: 698737(MICAL1) 701439 710292(MICAL3)
MCF: 102124064(MICAL2) 102135771(MICAL3) 102139619(MICAL1)
CSAB: 103222878(MICAL3) 103240067(MICAL2) 103240597(MICAL1)
RRO: 104668570(MICAL2) 104668827(MICAL1) 104676297(MICAL3)
RBB: 108533801(MICAL1) 108535533(MICAL2) 108541715(MICAL3)
CJC: 100394528(MICAL2) 100395442(MICAL1) 100400227(MICAL3)
SBQ: 101027900(MICAL2) 101040006(MICAL3) 101042616(MICAL1)
MMU: 171580(Mical1) 194401(Mical3) 320878(Mical2)
RNO: 294520(Mical1) 362427(Mical3) 365352(Mical2)
MUN: 110541039(Mical2) 110546057(Mical3) 110555442(Mical1)
CGE: 100752579(Mical1) 100754319(Mical3) 100763886(Mical2)
NGI: 103745899(Mical3) 103749557(Mical1) 103750410(Mical2)
HGL: 101701740(Mical1) 101709910(Mical3) 101712859(Mical2)
CCAN: 109684398 109684399 109688102(Mical2) 109694751(Mical1)
OCU: 100347412(MICAL1) 100353897(MICAL2) 100354238(MICAL3)
TUP: 102480907(MICAL3) 102486503(MICAL1) 102494378(MICAL2)
CFA: 476858(MICAL2) 481958(MICAL1) 486761(MICAL3)
AML: 100465187(MICAL3) 100465934(MICAL2) 100478759(MICAL1)
UMR: 103675754(MICAL3) 103677024(MICAL1) 103682359(MICAL2)
ORO: 101366928(MICAL2) 101371709(MICAL3) 101380122(MICAL1)
FCA: 101081558(MICAL3) 101091046 101100722(MICAL1)
PTG: 102951530(MICAL3) 102962220(MICAL1) 102962641(MICAL2)
AJU: 106968456(MICAL3) 106968890(MICAL1) 106971840
BTA: 508306(MICAL1) 532244(MICAL3) 534041(MICAL2)
BOM: 102266509(MICAL2) 102273179(MICAL1) 102286656(MICAL3)
BIU: 109559784(MICAL3) 109563835(MICAL1) 109569548(MICAL2)
PHD: 102319362(MICAL2) 102320915(MICAL3) 102342103(MICAL1)
CHX: 102172084(MICAL2) 102178522(MICAL3) 102185000(MICAL1)
OAS: 101113940(MICAL1) 101118097(MICAL3) 101121880(MICAL2)
SSC: 100153346(MICAL1) 100157721(MICAL3) 100519075(MICAL2)
CFR: 102507940(MICAL2) 102511399(MICAL1) 102522111(MICAL3)
CDK: 105086699(MICAL2) 105097110(MICAL1) 105100736(MICAL3)
BACU: 103000884(MICAL1) 103012504(MICAL3) 103012861(MICAL2)
LVE: 103069359(MICAL2) 103087508(MICAL1) 103088697(MICAL3)
OOR: 101275370(MICAL2) 101275745(MICAL3) 101285142(MICAL1)
DLE: 111164423(MICAL1) 111165227(MICAL2) 111176586(MICAL3)
ECB: 100054807(MICAL3) 100055978 100066627(MICAL1)
EPZ: 103551111(MICAL1) 103565092(MICAL3) 103566075
EAI: 106829758(MICAL1) 106833091(MICAL2) 106839442(MICAL3)
MYB: 102248958(MICAL2) 102251692(MICAL1) 102258499(MICAL3)
MYD: 102751644(MICAL3) 102759552(MICAL1) 102769793(MICAL2)
MNA: 107535318(MICAL2) 107537711(MICAL3) 107540706(MICAL1)
HAI: 109373294(MICAL3) 109388271 109388272 109391423(MICAL1)
RSS: 109446180 109449085(MICAL1) 109461201(MICAL3)
PALE: 102887125(MICAL3) 102889705(MICAL2) 102892071(MICAL1)
LAV: 100661909(MICAL3) 100663700 100665365(MICAL1)
MDO: 100022040(MICAL3) 100029104(MICAL1) 100030142(MICAL2)
SHR: 100920737(MICAL1) 100922670(MICAL2) 100929940(MICAL3)
PCW: 110218729(MICAL3) 110218891(MICAL2) 110222901(MICAL1)
OAA: 100085361(MICAL1) 100086054(MICAL3)
GGA: 418164(MICAL3) 423031(MICAL2) 429474(MICAL1)
MGP: 100541969 100545712(MICAL1) 100548226(MICAL3) 104911003(MICAL2) 104912086
CJO: 107314574 107314988(MICAL3) 107324612(MICAL1)
APLA: 101794484(MICAL2) 101803780(MICAL1) 101805047(MICAL3)
ACYG: 106030921(MICAL3) 106040898(MICAL2) 106040899(MICALCL) 106046127
TGU: 100219168(MICAL3) 100227450(MICAL2) 100232670(MICAL1)
LSR: 110469104(MICAL2) 110469123(MICALCL) 110473459(MICAL1) 110482582(MICAL3)
SCAN: 103820469(MICAL3) 103823343(MICAL1) 103824313(MICAL2)
GFR: 102032677(MICAL3) 102033849(MICAL2) 102034019(MICALCL) 102043330(MICAL1)
FAB: 101811190(MICAL2) 101819614(MICAL3) 101820098(MICAL1) 101821255(MICALCL)
PHI: 102107564(MICAL3) 102107919(MICAL2) 102108109(MICALCL) 102108714(MICAL1)
PMAJ: 107204329(MICAL3) 107205792(MICAL2) 107214787(MICAL1)
CCAE: 111928083(MICAL3) 111930849(MICAL2) 111939748(MICAL1)
CCW: 104692251(MICAL3) 104692546(MICAL1) 104692969
ETL: 114056527(MICAL2) 114065769(MICAL1) 114068977(MICAL3)
FPG: 101914505(MICAL3) 101922313(MICAL1) 101924713(MICAL2) 101924875(MICALCL)
FCH: 102047357(MICAL3) 102052557(MICAL2) 102060220(MICAL1)
CLV: 102085132(MICAL2) 102093478(MICAL3) 102096460(MICAL1)
EGZ: 104123406(MICAL2) 104123414(MICALCL) 104125757(MICAL1) 104134379(MICAL3)
NNI: 104009445(MICAL2) 104009522(MICALCL) 104013230(MICAL1) 104020203(MICAL3)
ACUN: 113481325(MICAL2) 113489033(MICAL1) 113491284(MICAL3)
AAM: 106487711(MICAL1) 106488631(MICAL3) 106497117(MICAL2) 106497118(MICALCL)
ASN: 102371980 102375438(MICAL3) 102377820(MICAL2)
AMJ: 102565781(MICAL1) 102574107(MICALCL) 106737063(MICAL3) 109280514
PSS: 102443943(MICAL3) 102447311 102462749(MICAL2)
CMY: 102932362(MICAL2) 102934250(MICALCL) 102935764(MICAL1) 102942117(MICAL3)
CPIC: 101936236(MICAL1) 101936780(MICAL3) 101946730(MICAL2)
ACS: 100552986(mical1) 100554427(mical3) 100564862(mical2) 103279667(micalcl)
PVT: 110076703(MICAL3) 110079674(MICAL1) 110080915(MICAL2) 110080917(MICALCL)
PMUR: 107289040(MICAL1) 107292277(MICAL3) 107293552 107299372
XTR: 100486205(mical3) 100487018(mical2)
NPR: 108785688(MICALCL) 108786140(MICAL2) 108791576(MICAL3)
DRE: 402881(mical3a) 566850(mical3b) 568573(mical1) 569564(mical2b) 569607(mical2a)
HCQ: 109512974 109520707(micalcl) 109520720 109525935(mical2) 109530374(mical3) 109532568
LCM: 102345089 102360081(MICAL3) 102366021(MICAL2) 106704417(MICALCL)
CIN: 100175527
SPU: 582210(MICAL3)
APLC: 110989175
DME: Dmel_CG33208(Mical)
DER: 6552049
DPE: 6598648
DSI: Dsimw501_GD20744(Dsim_GD20744)
DWI: 6647152
DAZ: 108615232
DNV: 108658541
MDE: 101887289
AAG: 5568484
AME: 413310
BIM: 100744389
BTER: 100650962
SOC: 105204672
AEC: 105145217
ACEP: 105617147
PBAR: 105423576
DQU: 106741595
CFO: 105257878
LHU: 105675006
PGC: 109854698
OBO: 105283811
PCF: 106785377
NVI: 100120752
MDL: 103579831
TCA: 100141546
NVL: 108557182
BMOR: 101742566(T15.180a)
PMAC: 106717230
PRAP: 111003396
HAW: 110382349
TNL: 113503945
API: 100168266
DNX: 107162729
CLEC: 106674386
ZNE: 110827699
TUT: 107372243
DPTE: 113794869
BMY: Bm1_43575
CRG: 105346067
OBI: 106870794
LAK: 106178150
SHX: MS3_03588
EGL: EGR_02335
ADF: 107332908
SMIN: v1.2.023094.t1(symbB.v1.2.023094.t1) v1.2.023094.t2(symbB.v1.2.023094.t2) v1.2.027574.t1(symbB.v1.2.027574.t1)
SPAR: SPRG_02957
 » show all
Taxonomy
Reference
1  [PMID:20148037]
  Authors
Hung RJ, Yazdani U, Yoon J, Wu H, Yang T, Gupta N, Huang Z, van Berkel WJ, Terman JR
  Title
Mical links semaphorins to F-actin disassembly.
  Journal
Nature 463:823-7 (2010)
DOI:10.1038/nature08724
  Sequence
Reference
2  [PMID:22116028]
  Authors
Hung RJ, Pak CW, Terman JR
  Title
Direct redox regulation of F-actin assembly and disassembly by Mical.
  Journal
Science 334:1710-3 (2011)
DOI:10.1126/science.1211956
  Sequence
Reference
3  [PMID:24212093]
  Authors
Hung RJ, Spaeth CS, Yesilyurt HG, Terman JR
  Title
SelR reverses Mical-mediated oxidation of actin to regulate F-actin dynamics.
  Journal
Nat Cell Biol 15:1445-54 (2013)
DOI:10.1038/ncb2871
  Sequence
Reference
4  [PMID:21864500]
  Authors
Zucchini D, Caprini G, Pasterkamp RJ, Tedeschi G, Vanoni MA
  Title
Kinetic and spectroscopic characterization of the putative monooxygenase domain of human MICAL-1.
  Journal
Arch Biochem Biophys 515:1-13 (2011)
DOI:10.1016/j.abb.2011.08.004
  Sequence
[hsa:64780]
Reference
5  [PMID:26845023]
  Authors
Vitali T, Maffioli E, Tedeschi G, Vanoni MA
  Title
Properties and catalytic activities of MICAL1, the flavoenzyme involved in cytoskeleton dynamics, and modulation by its CH, LIM and C-terminal domains.
  Journal
Arch Biochem Biophys 593:24-37 (2016)
DOI:10.1016/j.abb.2016.01.016
  Sequence
[hsa:64780]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.225
IUBMB Enzyme Nomenclature: 1.14.13.225
ExPASy - ENZYME nomenclature database: 1.14.13.225
BRENDA, the Enzyme Database: 1.14.13.225

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