Entry |
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Name |
tert-butyl alcohol monooxygenase;
mdpJK (gene names);
tert-butanol monooxygenase
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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Sysname |
tert-butyl alcohol,NADPH:oxygen oxidoreductase
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Reaction(IUBMB) |
tert-butyl alcohol + NADPH + H+ + O2 = 2-methylpropane-1,2-diol + NADP+ + H2O [RN: R11447]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
The enzyme, characterized from the bacterium Aquincola tertiaricarbonis, is a Rieske nonheme mononuclear iron oxygenase. It can also act, with lower efficiency, on propan-2-ol, converting it to propane-1,2-diol. Depending on the substrate, the enzyme also catalyses EC 1.14.19.48, tert-amyl alcohol desaturase.
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History |
EC 1.14.13.229 created 2016
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Orthology |
K21323 | tert-butyl alcohol monooxygenase / tert-amyl alcohol desaturase |
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Genes |
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Reference |
1 |
Authors |
Schafer, F., Breuer, U., Benndorf, D., von Bergen, M., Harms, H. and Muller, R.H. |
Title |
Growth of Aquincola tertiaricarbonis L108 on tert-butyl alcohol leads to the induction of a phthalate dioxygenase-related protein and its associated oxidoreductase subunit. |
Journal |
Eng Life Sci 7:512-519 (2007) |
Reference |
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Authors |
Schuster J, Schafer F, Hubler N, Brandt A, Rosell M, Hartig C, Harms H, Muller RH, Rohwerder T |
Title |
Bacterial degradation of tert-amyl alcohol proceeds via hemiterpene 2-methyl-3-buten-2-ol by employing the tertiary alcohol desaturase function of the Rieske nonheme mononuclear iron oxygenase MdpJ. |
Journal |
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Sequence |
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Other DBs |
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