KEGG   ENZYME: 1.14.13.229
Entry
EC 1.14.13.229              Enzyme                                 

Name
tert-butyl alcohol monooxygenase;
mdpJK (gene names);
tert-butanol monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
tert-butyl alcohol,NADPH:oxygen oxidoreductase
Reaction(IUBMB)
tert-butyl alcohol + NADPH + H+ + O2 = 2-methylpropane-1,2-diol + NADP+ + H2O [RN:R11447]
Reaction(KEGG)
R11447
Substrate
tert-butyl alcohol [CPD:C21389];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2-methylpropane-1,2-diol [CPD:C21290];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Aquincola tertiaricarbonis, is a Rieske nonheme mononuclear iron oxygenase. It can also act, with lower efficiency, on propan-2-ol, converting it to propane-1,2-diol. Depending on the substrate, the enzyme also catalyses EC 1.14.19.48, tert-amyl alcohol desaturase.
History
EC 1.14.13.229 created 2016
Orthology
K21323  tert-butyl alcohol monooxygenase / tert-amyl alcohol desaturase
Genes
MPT: Mpe_B0555
Reference
1
  Authors
Schafer, F., Breuer, U., Benndorf, D., von Bergen, M., Harms, H. and Muller, R.H.
  Title
Growth of Aquincola tertiaricarbonis L108 on tert-butyl alcohol leads to the induction of a phthalate dioxygenase-related protein and its associated oxidoreductase subunit.
  Journal
Eng Life Sci 7:512-519 (2007)
Reference
2  [PMID:22194447]
  Authors
Schuster J, Schafer F, Hubler N, Brandt A, Rosell M, Hartig C, Harms H, Muller RH, Rohwerder T
  Title
Bacterial degradation of tert-amyl alcohol proceeds via hemiterpene 2-methyl-3-buten-2-ol by employing the tertiary alcohol desaturase function of the Rieske nonheme mononuclear iron oxygenase MdpJ.
  Journal
J Bacteriol 194:972-81 (2012)
DOI:10.1128/JB.06384-11
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.229
IUBMB Enzyme Nomenclature: 1.14.13.229
ExPASy - ENZYME nomenclature database: 1.14.13.229
BRENDA, the Enzyme Database: 1.14.13.229

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Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (838)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (2)   
   RefGene (835)   
All databases (847)   

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