KEGG   ENZYME: 1.14.13.229Help
Entry
EC 1.14.13.229              Enzyme                                 

Name
tert-butyl alcohol monooxygenase;
mdpJK (gene names);
tert-butanol monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
tert-butyl alcohol,NADPH:oxygen oxidoreductase
Reaction(IUBMB)
tert-butyl alcohol + NADPH + H+ + O2 = 2-methylpropane-1,2-diol + NADP+ + H2O [RN:R11447]
Reaction(KEGG)
Substrate
tert-butyl alcohol [CPD:C21389];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2-methylpropane-1,2-diol [CPD:C21290];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Aquincola tertiaricarbonis, is a Rieske nonheme mononuclear iron oxygenase. It can also act, with lower efficiency, on propan-2-ol, converting it to propane-1,2-diol. Depending on the substrate, the enzyme also catalyses EC 1.14.19.48, tert-amyl alcohol desaturase.
History
EC 1.14.13.229 created 2016
Orthology
K21323  tert-butyl alcohol monooxygenase / tert-amyl alcohol desaturase
Genes
MPT: Mpe_B0555
Taxonomy
Reference
1
  Authors
Schafer, F., Breuer, U., Benndorf, D., von Bergen, M., Harms, H. and Muller, R.H.
  Title
Growth of Aquincola tertiaricarbonis L108 on tert-butyl alcohol leads to the induction of a phthalate dioxygenase-related protein and its associated oxidoreductase subunit.
  Journal
Eng Life Sci 7:512-519 (2007)
Reference
2  [PMID:22194447]
  Authors
Schuster J, Schafer F, Hubler N, Brandt A, Rosell M, Hartig C, Harms H, Muller RH, Rohwerder T
  Title
Bacterial degradation of tert-amyl alcohol proceeds via hemiterpene 2-methyl-3-buten-2-ol by employing the tertiary alcohol desaturase function of the Rieske nonheme mononuclear iron oxygenase MdpJ.
  Journal
J Bacteriol 194:972-81 (2012)
DOI:10.1128/JB.06384-11
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.229
IUBMB Enzyme Nomenclature: 1.14.13.229
ExPASy - ENZYME nomenclature database: 1.14.13.229
BRENDA, the Enzyme Database: 1.14.13.229

  All links  
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (838)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (2)   
   RefGene (835)   
All databases (847)   

Download RDF
DBGET integrated database retrieval system