KEGG   ENZYME: 1.14.13.232Help
Entry
EC 1.14.13.232              Enzyme                                 

Name
6-methylpretetramide 4-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
6-methylpretetramide,NADPH:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
6-methylpretetramide + NADPH + H+ + O2 = 4-hydroxy-6-methylpretetramide + NADP+ + H2O [RN:R05463]
Reaction(KEGG)
R05463;
(other) R09190
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Substrate
6-methylpretetramide [CPD:C06629];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
4-hydroxy-6-methylpretetramide [CPD:C06628];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. That bacterium possesses two enzymes that can catalyse the reaction - OxyE is the main isozyme, while OxyL has a lower activity. OxyL is bifunctional, and its main function is EC 1.14.13.233, 4-hydroxy-6-methylpretetramide 12a-monooxygenase. Contains FAD.
History
EC 1.14.13.232 created 2016
Pathway
ec00253  Tetracycline biosynthesis
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K14252  6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase
K14253  6-methylpretetramide 4-monooxygenase
Genes
SVL: Strvi_8156
SFA: Sfla_5329
SBH: SBI_06856 SBI_06866
SHY: SHJG_1193
SHO: SHJGH_1028
STRP: F750_1294
SCI: B446_13370 B446_13470
SRC: M271_30845
SPRI: SPRI_0201 SPRI_0276 SPRI_7077 SPRI_7152
SPAV: Spa2297_32735 Spa2297_32760
 » show all
Taxonomy
Reference
1  [PMID:18422316]
  Authors
Zhang W, Watanabe K, Cai X, Jung ME, Tang Y, Zhan J.
  Title
Identifying the minimal enzymes required for anhydrotetracycline biosynthesis.
  Journal
J Am Chem Soc 130:6068-9 (2008)
DOI:10.1021/ja800951e
Reference
2  [PMID:19472250]
  Authors
Wang P, Zhang W, Zhan J, Tang Y.
  Title
Identification of OxyE as an ancillary oxygenase during tetracycline biosynthesis.
  Journal
Chembiochem 10:1544-50 (2009)
DOI:10.1002/cbic.200900122
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.232
IUBMB Enzyme Nomenclature: 1.14.13.232
ExPASy - ENZYME nomenclature database: 1.14.13.232
BRENDA, the Enzyme Database: 1.14.13.232

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