KEGG   ENZYME: 1.14.13.235Help
Entry
EC 1.14.13.235              Enzyme                                 

Name
indole-3-acetate monooxygenase;
iacA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(indol-3-yl)acetate,NADH:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
(indol-3-yl)acetate + NADH + H+ + O2 = (2-hydroxy-1H-indol-3-yl)acetate + NAD+ + H2O [RN:R11730]
Reaction(KEGG)
Substrate
(indol-3-yl)acetate [CPD:C00954];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(2-hydroxy-1H-indol-3-yl)acetate [CPD:C21619];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme, characterized from Pseudomonas putida strains, catalyses the first step in a pathway for degradation of the plant hormone indole-3-acetate. When acting on indole, the enzyme forms indoxyl, which reacts spontaneously with oxygen to form the blue dye indigo.
History
EC 1.14.13.235 created 2017
Orthology
K22027  indole-3-acetate monooxygenase
Genes
EFE: EFER_0795
EAS: Entas_2482
EAE: EAE_02315
EAR: CCG33524
ROR: RORB6_23125
RON: TE10_23150
RPLN: B1209_04450
RAO: DSD31_04580
KGO: CEW81_10430
KIE: NCTC12125_01038(hsaA_1)
EBF: D782_1303
PVA: Pvag_pPag20222(mmfH)
PMK: MDS_4199
PCQ: PcP3B5_30190(hsaA_2)
PEN: PSEEN4759
PKC: PKB_2661
ACB: A1S_1864
ABY: ABAYE1701
ABN: AB57_2198
ABX: ABK1_2443
ABH: M3Q_2323
ABAD: ABD1_18860
ABAZ: P795_7525
ABAU: IX87_20780
ABAA: IX88_11495
MMAI: sS8_5028
HAM: HALO0116
RSO: RSp0219(ncnH)
RSE: F504_3657
BTQ: BTQ_3617
BTJ: BTJ_4653
BTZ: BTL_5441
BTV: BTHA_4762
BTHE: BTN_5111
BTHM: BTRA_5380
BTHA: DR62_5241
BTHL: BG87_5447
BOK: DM82_6331
BOC: BG90_4343
BCEW: DM40_5613
BAM: Bamb_5351
BCED: DM42_4446
BCON: NL30_22630
BLAT: WK25_18275
BSEM: WJ12_23190
BMEC: WJ16_22210
BGU: KS03_3547
BXE: Bxe_B2308
BXB: DR64_4640
BFN: OI25_3707
PSPU: NA29_23275
PLG: NCTC10937_03009(hsaA_1)
HSE: Hsero_1346(ncnH) Hsero_1489(ncnH)
HRB: Hrubri_1157(ncnH) Hrubri_1339(ncnH)
MXA: MXAN_0950
CCRO: CMC5_036220(fadE)
HOH: Hoch_6104
PLA: Plav_1367
RBS: RHODOSMS8_02734(hsaA)
BJA: bll2063(nrgC) blr5615 blr8155
BBT: BBta_2555
BRAD: BF49_3936
RPB: RPB_3894
RBM: TEF_13695
HNE: HNE_3277
NAR: Saro_3809
SPHP: LH20_14665
SPHD: HY78_23005
SSAN: NX02_01785
SFLA: SPHFLASMR4Y_03250(hsaA)
BLAS: BSY18_169
ASZ: ASN_1346(nrgC) ASN_2584
MTU: Rv3094c
MTC: MT3178
MRA: MRA_3126
MTUR: CFBS_3263
MTD: UDA_3094c
MTUC: J113_21595
MTUE: J114_16555
MTUL: TBHG_03024
MTUT: HKBT1_3250
MTUU: HKBT2_3255
MBB: BCG_3119c
MBT: JTY_3114
MBX: BCGT_2945
MAF: MAF_31010
MIT: OCO_14870
MIA: OCU_15320
MUL: MUL_3474
MJL: Mjls_4200
MMI: MMAR_1556
MVQ: MYVA_4986
CGT: cgR_2293
CAMG: CAMM_01480
NFA: NFA_28430
NFR: ERS450000_01246(hsaA_4)
ROP: ROP_25640
GOR: KTR9_1756
GOM: D7316_03262(hsaA_3)
SBH: SBI_09701
SHY: SHJG_7330
STRE: GZL_02224
SMAL: SMALA_7733
CFL: Cfla_3621
NDA: Ndas_1183
FAL: FRAAL1671
SACC: EYD13_11945(hsaA)
AMD: AMED_0843
AMN: RAM_04305
AMM: AMES_0841
AMZ: B737_0842
AOI: AORI_4212
SESP: BN6_64260
KAL: KALB_5619
ACAD: UA74_15780
ACTN: L083_8079
GBA: J421_1886
MPW: MPR_3152
WIN: WPG_3254
 » show all
Taxonomy
Reference
1  [PMID:15870323]
  Authors
Leveau JH, Lindow SE
  Title
Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290.
  Journal
Appl Environ Microbiol 71:2365-71 (2005)
DOI:10.1128/AEM.71.5.2365-2371.2005
  Sequence
Reference
2  [PMID:23881445]
  Authors
Scott JC, Greenhut IV, Leveau JH
  Title
Functional characterization of the bacterial iac genes for degradation of the plant hormone indole-3-acetic acid.
  Journal
J Chem Ecol 39:942-51 (2013)
DOI:10.1007/s10886-013-0324-x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.235
IUBMB Enzyme Nomenclature: 1.14.13.235
ExPASy - ENZYME nomenclature database: 1.14.13.235
BRENDA, the Enzyme Database: 1.14.13.235

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