Entry
Name
indole-3-acetate monooxygenase;
iacA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(indol-3-yl)acetate,NADH:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
(indol-3-yl)acetate + NADH + H+ + O2 = (2-hydroxy-1H-indol-3-yl)acetate + NAD+ + H2O [RN:
R11730 ]
Reaction(KEGG)
Substrate
Product
Comment
The enzyme, characterized from Pseudomonas putida strains, catalyses the first step in a pathway for degradation of the plant hormone indole-3-acetate. When acting on indole, the enzyme forms indoxyl, which reacts spontaneously with oxygen to form the blue dye indigo.
History
EC 1.14.13.235 created 2017
Orthology
K22027 indole-3-acetate monooxygenase
Genes
KIE : NCTC12125_01038(hsaA_1)
PCQ : PcP3B5_30190(hsaA_2)
PPZ : H045_08875 H045_09020
PMUD : NCTC8068_03343(hsaA_3)
PSAM : HU731_027010 HU731_027170
ACAL : BUM88_06985 BUM88_10705
BCEN : DM39_4513 DM39_4924
BMJ : BMULJ_03433 BMULJ_03439
BMUL : NP80_3640 NP80_3646
BTEI : WS51_01695 WS51_01725
BPSL : WS57_10705 WS57_12540
BGF : BC1003_3875 BC1003_3884
BYI : BYI23_A013760 BYI23_B005930
BPY : Bphyt_2161 Bphyt_6111
BCAI : K788_0003940 K788_0005052
PHS : C2L64_07330 C2L64_36230
PTER : C2L65_07065 C2L65_31440
PSAA : QEN71_07515 QEN71_31130
PLAD : PPGU16_14130(nrgC) PPGU16_74080
PAZZ : PBP221_15240 PBP221_77740
PLG : NCTC10937_03009(hsaA_1)
BUE : BRPE67_ACDS14180 BRPE67_BCDS08210
HSE : Hsero_1346(ncnH) Hsero_1489(ncnH)
HSZ : ACP92_06750 ACP92_07480
HRB : Hrubri_1157(ncnH) Hrubri_1339(ncnH)
HHF : E2K99_06605 E2K99_07530
HFR : G5S34_06960 G5S34_08265
HEW : HBDW_12120 HBDW_13850
RBS : RHODOSMS8_02734(hsaA)
CAUB : AMEJIAPC_00981(hsaA)
PALW : PSAL_015710(hsaA_1) PSAL_021730(hsaA_2)
MANH : LA6_001807(hsaA_1) LA6_005347(hsaA_3)
SWI : Swit_0738 Swit_4184 Swit_4295
SFLA : SPHFLASMR4Y_03250(hsaA)
ASZ : ASN_1346(nrgC) ASN_2584
AACE : A0U92_02665 A0U92_15820
MBD : MEBOL_006408 MEBOL_006972
BFD : NCTC4823_00654(hsaA_1)
MPAG : C0J29_03260 C0J29_08835
MBAI : MB901379_01378(hsaA_1)
NFR : ERS450000_01246(hsaA_4)
ARHD : VSH64_04755 VSH64_46815
AMOG : QRX60_01190 QRX60_07105 QRX60_36230 QRX60_45065
ANW : QP939_01320 QP939_08360
PSDS : K1T35_01070 K1T35_34935
MCAB : HXZ27_16010 HXZ27_16015
NHY : JQS43_08645 JQS43_08650
» show all
Taxonomy
Reference
Authors
Leveau JH, Lindow SE
Title
Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290.
Journal
Sequence
Reference
Authors
Scott JC, Greenhut IV, Leveau JH
Title
Functional characterization of the bacterial iac genes for degradation of the plant hormone indole-3-acetic acid.
Journal
Other DBs