KEGG   ENZYME: 1.14.13.235
Entry
EC 1.14.13.235              Enzyme                                 
Name
indole-3-acetate monooxygenase;
iacA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(indol-3-yl)acetate,NADH:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
(indol-3-yl)acetate + NADH + H+ + O2 = (2-hydroxy-1H-indol-3-yl)acetate + NAD+ + H2O [RN:R11730]
Reaction(KEGG)
R11730
Substrate
(indol-3-yl)acetate [CPD:C00954];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(2-hydroxy-1H-indol-3-yl)acetate [CPD:C21619];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme, characterized from Pseudomonas putida strains, catalyses the first step in a pathway for degradation of the plant hormone indole-3-acetate. When acting on indole, the enzyme forms indoxyl, which reacts spontaneously with oxygen to form the blue dye indigo.
History
EC 1.14.13.235 created 2017
Orthology
K22027  indole-3-acetate monooxygenase
Genes
EFE: EFER_0795
EAS: Entas_2482
EAE: EAE_02315
EAR: CCG33524
ROR: RORB6_23125
RON: TE10_23150
RPLN: B1209_04450
RAO: DSD31_04580
KGO: CEW81_10430
KIE: NCTC12125_01038(hsaA_1)
KAS: KATP_26500
KLU: K7B04_19210
LAX: APT61_09000
LEI: C2U54_18520
LEH: C3F35_21230
LEE: DVA44_09630
LER: GNG29_13055
LEA: GNG26_13325
LEY: DVA43_04225
LEF: LJPFL01_2408
LEW: DAI21_14150
BUF: D8682_22140
BFT: MNO13_06485
SGOE: A8O29_009730
IZH: FEM41_10700
PSGC: G163CM_02870(hsaA)
EBF: D782_1303
XTN: FD63_14490
XHY: FZ025_04605
PMK: MDS_4199
PRE: PCA10_14730
PFUW: KF707C_41600
PCQ: PcP3B5_30190(hsaA_2)
PMUI: G4G71_17720
PNT: G5B91_14505
PPG: PputGB1_2207
PPZ: H045_08875 H045_09020
PVR: PverR02_09810
PMUD: NCTC8068_03343(hsaA_3)
PKC: PKB_2661
PSW: LK03_16020
PPSY: AOC04_11320
PPSL: BJP27_02180
PYM: AK972_2155
PUM: HGP31_18345
PLAL: FXN65_06690
PSEP: C4K39_4234
PSAM: HU731_027010 HU731_027170
PASG: KSS96_11745
PTW: TUM18999_33760
PIZ: LAB08_R20910
PKM: PZ739_16485
PNN: KEM63_12330
ACB: A1S_1864
ABY: ABAYE1701
ABC: ACICU_01975
ABN: AB57_2198
ABB: ABBFA_01570(hsaA)
ABX: ABK1_2443
ABZ: ABZJ_02156
ABR: ABTJ_01733
ABD: ABTW07_2187
ABH: M3Q_2323
ABAD: ABD1_18860
ABJ: BJAB07104_01764
ABAB: BJAB0715_02121
ABAJ: BJAB0868_02114
ABAZ: P795_7525
ABK: LX00_09995
ABAU: IX87_20780
ABAA: IX88_11495
ABW: BL01_00725
ABAL: ABLAC_27380
ACC: BDGL_001343
ANO: RR32_08530
ALC: OTEC02_08160
ACAL: BUM88_06985 BUM88_10705
ASEI: I8T81_08695
ACD: AOLE_08525
ASOL: BEN76_04970
ADV: DJ533_11640
ARJ: DOM24_03915
AGU: AS4_15380
ADS: FPL17_09150
ABER: BSR55_08050
ATN: FM020_06540
AGYL: FPL18_01520
ACIU: A3K93_14200
ATZ: M5E07_06770
APRL: PY247_12060
ACOI: NQU59_16850
AVIV: LF296_05135
ALR: DS731_04875
MAH: MEALZ_3790
MMAI: sS8_5028
HAK: KO116_00201
HAM: HALO0116
HHH: CLM76_06875
HTT: HZS52_05460
HPRO: LMS44_23090
MMW: Mmwyl1_1823
MME: Marme_3620
MGEO: CFI10_04615
MSEC: LN244_11550
VMS: LVJ82_03825
RSO: RSp0219(ncnH)
RSE: F504_3657
RPU: CDC45_18710
RNC: GO999_23100
BTE: BTH_II0323
BTQ: BTQ_3617
BTJ: BTJ_4653
BTZ: BTL_5441
BTV: BTHA_4762
BTHE: BTN_5111
BTHM: BTRA_5380
BTHA: DR62_5241
BTHL: BG87_5447
BOK: DM82_6331
BOC: BG90_4343
BUR: Bcep18194_B2500
BCM: Bcenmc03_3905
BCH: Bcen2424_3615
BCJ: BCAM0644 BCAM2114
BCEN: DM39_4513 DM39_4924
BCEW: DM40_5613
BCEO: I35_4540 I35_5996
BAM: Bamb_5351
BAC: BamMC406_3521
BMJ: BMULJ_03433 BMULJ_03439
BMU: Bmul_5077 Bmul_5083
BMK: DM80_4253 DM80_4260
BMUL: NP80_3640 NP80_3646
BCED: DM42_4446
BCEP: APZ15_21240
BPYR: ABD05_18650
BCON: NL30_22630
BLAT: WK25_18275
BTEI: WS51_01695 WS51_01725
BSEM: WJ12_23190
BPSL: WS57_10705 WS57_12540
BMEC: WJ16_22210
BSTL: BBJ41_30855
BANN: JFN94_20730
BARI: NLX30_19555
BGL: bglu_2g00910
BGU: KS03_3547
BGF: BC1003_3875 BC1003_3884
BGD: bgla_1g15260
BYI: BYI23_A013760 BYI23_B005930
BUE: BRPE67_ACDS14180 BRPE67_BCDS08210
BUQ: AC233_26785
BUM: AXG89_31775
BURK: DM992_28755
BXE: Bxe_B2308
BXB: DR64_4640
BGE: BC1002_5124
BPY: Bphyt_2161 Bphyt_6111
BFN: OI25_3707
BCAI: K788_0003940 K788_0005052
PARB: CJU94_31910
PHS: C2L64_07330 C2L64_36230
PTER: C2L65_07065 C2L65_31440
PGP: CUJ91_29450
PCAF: DSC91_002778
PGIS: I6I06_03815
PACS: FAZ98_33420
PEW: KZJ38_25175
PDIO: PDMSB3_0784.1
PSPU: NA29_23275
PLG: NCTC10937_03009(hsaA_1)
CABA: SBC2_82010(hsaA_2)
CDN: BN940_15426
PBH: AAW51_0755
ATER: MW290_06685
HSE: Hsero_1346(ncnH) Hsero_1489(ncnH)
HSZ: ACP92_06750 ACP92_07480
HRB: Hrubri_1157(ncnH) Hrubri_1339(ncnH)
HHF: E2K99_06605 E2K99_07530
HFR: G5S34_06960 G5S34_08265
MALI: EYF70_08040
TMJ: P0M04_17670
THK: CCZ27_20550
PLA: Plav_1367
PMOB: HG718_14205
RBS: RHODOSMS8_02734(hsaA)
NEO: CYG48_20570
BJA: bll2063(nrgC)
BJU: BJ6T_77930
BJP: RN69_37830
BRK: CWS35_13215
BOT: CIT37_02690
BARH: WN72_10815
BVZ: BRAD3257_7082(nrgC)
BDG: LPJ38_35760
BSEP: HAP48_0034785
BBAN: J4G43_043620
RPB: RPB_3894
MIND: mvi_01170(nrgC)
MHEY: H2LOC_002660
MAAD: AZF01_21495
RBM: TEF_13695
CMB: CSW64_05340
CEH: CEW89_04600
OCD: FHY55_15890
TGL: HFZ77_09585
TMD: KUV46_10225
HAT: RC74_11685
GFU: KM031_19230
PSHQ: F3W81_00940
PALW: PSAL_015710(hsaA_1) PSAL_021730(hsaA_2)
HNE: HNE_3277
HBC: AEM38_04945
NAR: Saro_3809
NOR: FA702_19525
NDR: HT578_10900
SWI: Swit_0738 Swit_4184 Swit_4295
SPHD: HY78_23005
SPHG: AZE99_11865
SFLA: SPHFLASMR4Y_03250(hsaA)
SPHY: CHN51_10695
BLAS: BSY18_169
BFW: B5J99_09860
PARJ: J4G78_06065
PHAO: HF685_05875
NTD: EGO55_15640
GSP: IGS75_08905
APT: APA01_22070
APW: APA42C_22070
APF: APA03_22070
APU: APA07_22070
APG: APA12_22070
APQ: APA22_22070
APX: APA26_22070
APZ: APA32_22070
APK: APA386B_1067
ASZ: ASN_1346(nrgC) ASN_2584
AACE: A0U92_02665 A0U92_15820
ATO: CIW82_05335
AASC: A4S02_09380
ACEK: FLP30_08760
AZL: AZL_a08880
ALI: AZOLI_p10984
ATI: AL072_30315
MSD: MYSTI_01236
MFB: MFUL124B02_06905
SUR: STAUR_7920
AGE: AA314_06014
AVM: JQX13_44055
MBD: MEBOL_006408 MEBOL_006972
CFUS: CYFUS_003905
SCL: sce0600
SCU: SCE1572_04140
CCRO: CMC5_036220(fadE)
PAUU: E8A73_012995
MRM: A7982_07097
SAMY: DB32_001527
HOH: Hoch_6104
BFD: NCTC4823_00654(hsaA_1)
SCIA: HUG15_01500
NDT: L1999_05650
NNV: QNH39_24725
AIA: AWH56_018090
PBJ: VN24_18670
SPOS: DV702_11125
MTU: Rv3094c
MTV: RVBD_3094c
MTC: MT3178
MRA: MRA_3126
MTF: TBFG_13111
MTB: TBMG_00873
MTK: TBSG_00879
MTZ: TBXG_000865
MTG: MRGA327_19035
MTI: MRGA423_19290
MTE: CCDC5079_2851
MTUR: CFBS_3263
MTL: CCDC5180_2814
MTO: MTCTRI2_3157
MTD: UDA_3094c
MTN: ERDMAN_3386
MTUB: MT7199_3127
MTUC: J113_21595
MTUE: J114_16555
MTX: M943_15970
MTUL: TBHG_03024
MTUT: HKBT1_3250
MTUU: HKBT2_3255
MTQ: HKBS1_3261
MBO: BQ2027_MB3121C
MBB: BCG_3119c
MBT: JTY_3114
MBM: BCGMEX_3116c
MBK: K60_032110
MBX: BCGT_2945
MAF: MAF_31010
MMIC: RN08_3409
MCE: MCAN_31201
MCQ: BN44_60611
MCV: BN43_60087
MCX: BN42_41111
MCZ: BN45_60083
MSA: Mycsm_03956
MUL: MUL_3474
MMI: MMAR_1556
MMAE: MMARE11_14690
MLI: MULP_01687
MPSE: MPSD_42910
MSHO: MSHO_56680
MKN: MKAN_22615
MFJ: MFLOJ_23370
MSTO: MSTO_21420
MSAK: MSAS_27870
MKU: I2456_07795
MLW: MJO58_05545
MPAG: C0J29_03260 C0J29_08835
MGOR: H0P51_07820
MBAI: MB901379_01378(hsaA_1)
MSEO: MSEO_49800
MLJ: MLAC_30410
MSHJ: MSHI_23250
MSPG: F6B93_06370
MOT: LTS72_17650
MPAA: MKK62_24180
CGT: cgR_2293
CGS: C624_11710
CGG: C629_11720
CHN: A605_13580
CGY: CGLY_10715
CCJZ: ccrud_12910
CAMG: CAMM_01480
CSUR: N24_2482
CCOE: CETAM_09570(hsaA3)
COK: COCCU_13735(hsaA4)
NFA: NFA_28430
NFR: ERS450000_01246(hsaA_4)
RHA: RHA1_ro02839
ROP: ROP_25640
ROA: Pd630_LPD07002
RPY: Y013_03820
RBY: CEJ39_14460
RAV: AAT18_23085
RRZ: CS378_12830
RHQ: IM25_17665
RKO: JWS14_14955
ROZ: CBI38_04590
RPSK: JWS13_29915
GOR: KTR9_1756
GOQ: ACH46_11335
GTA: BCM27_09460
GOM: D7316_03262(hsaA_3)
GJI: H1R19_22315
GHN: MVF96_08805
SVL: Strvi_4798
SRC: M271_04310
SMAL: SMALA_7733
SSOI: I1A49_42455
SBH: SBI_09701
SLS: SLINC_7755
SPHV: F9278_44470
SBRO: GQF42_41595
SROI: IAG44_04100
SAOV: G3H79_34610
SSPN: LXH13_37995
LRP: MUN76_10955
AEZ: C3E78_16725
NOA: BKM31_25180
NGN: LCN96_21570
AMD: AMED_0843 AMED_1899
AMN: RAM_04305 RAM_09635
AMM: AMES_0841 AMES_1884
AMZ: B737_0842 B737_1885
AOI: AORI_4212
AJA: AJAP_17850
AMQ: AMETH_3582
AMYC: CU254_35900
AMYY: YIM_23125(hsaA3)
APRT: MUY14_32585
AROO: NQK81_44965
ATHM: L1857_18550
SESP: BN6_64260
SSYI: EKG83_36550
KUT: JJ691_06680
KPHY: AOZ06_24575
LED: BBK82_46665
PMAD: BAY61_14220
MCAB: HXZ27_16010 HXZ27_16015
ACTN: L083_8079
AFS: AFR_42250
NHY: JQS43_08645 JQS43_08650
BSOL: FSW04_19010
AYM: YM304_15660
EUZ: DVS28_a1817
TBH: Tbon_13090
TFLA: O0235_14210
DDR: Deide_1p01570
GBA: J421_1886
 » show all
Reference
1  [PMID:15870323]
  Authors
Leveau JH, Lindow SE
  Title
Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290.
  Journal
Appl Environ Microbiol 71:2365-71 (2005)
DOI:10.1128/AEM.71.5.2365-2371.2005
  Sequence
[ppg:PputGB1_2207]
Reference
2  [PMID:23881445]
  Authors
Scott JC, Greenhut IV, Leveau JH
  Title
Functional characterization of the bacterial iac genes for degradation of the plant hormone indole-3-acetic acid.
  Journal
J Chem Ecol 39:942-51 (2013)
DOI:10.1007/s10886-013-0324-x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.235
IUBMB Enzyme Nomenclature: 1.14.13.235
ExPASy - ENZYME nomenclature database: 1.14.13.235
BRENDA, the Enzyme Database: 1.14.13.235

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Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (4389)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (434)   
   KEGG MGENES (3281)   
   RefGene (673)   
Protein sequence (5)   
   UniProt (3)   
   SWISS-PROT (2)   
DNA sequence (2)   
   GenBank (1)   
   EMBL (1)   
Protein domain (6)   
   InterPro (6)   
All databases (4411)   

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