KEGG ENZYME: 1.14.13.24

ENZYME: 1.14.13.24

Entry

EC 1.14.13.24               Enzyme

Name

3-hydroxybenzoate 6-monooxygenase;
3-hydroxybenzoate 6-hydroxylase;
m-hydroxybenzoate 6-hydroxylase;
3-hydroxybenzoic acid-6-hydroxylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

3-hydroxybenzoate,NADH:oxygen oxidoreductase (6-hydroxylating)

Reaction(IUBMB)

3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O [RN:R02589]

Reaction(KEGG)

R02589

Substrate

3-hydroxybenzoate [CPD:C00587];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

2,5-dihydroxybenzoate [CPD:C00628];
NAD+ [CPD:C00003];
H2O [CPD:C00001]

Comment

A flavoprotein (FAD). Acts also on a number of analogues of 3-hydroxybenzoate substituted in the 2, 4, 5 and 6 positions; NADPH can act instead of NADH, but more slowly.

History

EC 1.14.13.24 created 1984

Pathway

ec00362

Benzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K22270

3-hydroxybenzoate 6-monooxygenase

Genes

ECE:

Z3390

ECS:

ECs_3027

ECF:	ECH74115_3267

ETW:

ECSP_3013

ELX:	CDCO157_2790

ECOO:

ECRM13514_5372

ECOH:

ECRM13516_3724

EOK:	G2583_2678

ELR:	ECO55CA74_13240

EAL:	EAKF1_ch3857

ERUY:

OSH18_20605

STY:

STY2405

STT:

t0680

SEX:	STBHUCCB_7280

SENT:

TY21A_03470

STM:

STM2175

SEO:	STM14_2682

SEV:	STMMW_22071

SEY:	SL1344_2152

SEM:	STMDT12_C21960

SEJ:	STMUK_2205

SEB:	STM474_2264

SEF:	UMN798_2349

SETU:

STU288_07260

SETC:

CFSAN001921_05910

SENR:

STMDT2_21521

SEND:

DT104_22351

SENI:

CY43_11700

SEEN:

SE451236_17080

SPT:

SPA0676

SEK:

SSPA0635

SPQ:	SPAB_00841

SEI:

SPC_1527

SEC:	SCH_2192(nhg)

SEH:	SeHA_C2410

SHB:

SU5_02767

SENH:

CFSAN002069_20950

SEEH:

SEEH1578_20150

SEE:	SNSL254_A2365

SENN:

SN31241_32870

SEW:	SeSA_A2414

SEA:	SeAg_B2321

SENS:

Q786_10795

SED:

SeD_A2522

SEG:

SG2211

SEL:

SPUL_0714

SEGA:

SPUCDC_0714

SET:

SEN2168

SENA:

AU38_10960

SENO:

AU37_10970

SENV:

AU39_10970

SENQ:

AU40_12270

SENL:

IY59_11265

SENJ:

CFSAN001992_00485

SEEC:

CFSAN002050_17880

SEEB:

SEEB0189_008635

SEEP:

I137_03245

SENB:

BN855_22640

SENE:

IA1_10880

SENC:

SEET0819_18050

SBG:

SBG_1987

SBZ:

A464_2299

SBV:	N643_09685

SALZ:

EOS98_08090

KQV:	B8P98_08965

KOX:

KOX_25675

KOE:

A225_4035

KOM:	HR38_23810

KOK:	KONIH1_19970

KOC:	AB185_16310

EAE:

EAE_23875

EAR:

CCG29101

KLL:	BJF97_19860

KLW:	DA718_09145

KAR:	LGL98_07905

KGR:	JJJ10_08755

KPAS:

LUW96_26395

KLC:	K7H21_08775

KLM:	BWI76_19570

KLP:	GUC22_08035

RON:	TE10_19725

RTG:	NCTC13098_02137(mhbM)

CRO:

ROD_22631

CKO:

CKO_00654

CFD:	CFNIH1_21965

CBRA:

A6J81_00350

CYO:	CD187_08425

CPOT:

FOB25_19515

CFQ:	C2U38_16180

CSED:

JY391_08185

CAMA:

F384_11420

CAF:	AL524_11385

CFAR:

CI104_16805

CIR:	C2U53_26655

CIE:	AN232_13145

CTEL:

GBC03_13665

CARS:

E1B03_17625

CIX:	M4I31_08310

CIB:	HF677_008440

CITR:

GUC46_08335

EBT:	EBL_c13690

PGE:	LG71_12560

KAS:	KATP_15130

KLU:	K7B04_21445

PSGC:

G163CM_04730(mhbM)

EBF:

D782_1486

SMAR:

SM39_2826(mhbM)

SMAC:

SMDB11_2639(mhbM)

SSAR:

SSARUM_003223

XBV:	XBW1_2121(mhbM)

PRAG:

EKN56_19220

LGB:	V8L73_01460

XHY:	FZ025_09535

PEN:	PSEEN2596(mhbM)

PCZ:	PCL1606_26180

PCHP:

C4K32_3541

PSEM:

TO66_18335

PIZ:	LAB08_R28900

PKK:	QQ992_14050

ALC:	OTEC02_09095

ASEI:

I8T81_09000

ACD:	AOLE_09105

AEI:	AOY20_07145

AWG:	GFH30_03355

ACIG:

BS636_05965

HAK:	KO116_03925

HAAH:

HALA3H3_370027(nagX)

HNP:	SR894_21660

MARS:

A8C75_13630

MRZ:	KDW95_19590

ASIM:

FE240_04935

OCE:

GU3_03005

ZDF:	AN401_12515

NAQ:	D0T90_04540

NLN:	ORY85_05790

VFF:	VITFI_CDS3374

VIT:	ADP71_15240

VST:	LVJ81_05800

VMS:	LVJ82_03170

PSE:

NH8B_2047

RPJ:	N234_25225 N234_25345

REH:	H16_B0876(h16_B0876)

CNC:	CNE_2c08340(mhbM) CNE_BB2p00890(xlnD)

CUH:	BJN34_30895

REU:	Reut_B5805 Reut_B5861

CBW:	RR42_s0703 RR42_s2029

CCUP:

BKK81_09165

CUP:	BKK80_09450

CUU:	BKK79_03670

COX:	E0W60_03835 E0W60_09680

CCAX:

KZ686_23590

CUK:	KB879_09140 KB879_37390

CNAN:

A2G96_24160

BUR:	Bcep18194_B1886

BCM:	Bcenmc03_3371

BCH:	Bcen2424_4148

BCJ:	BCAM1274(mhbM)

BCEN:

DM39_3727(mhbM)

BCEO:

I35_5123(mhbM)

BMJ:	BMULJ_04070(nahG)

BMU:

Bmul_4439

BMK:	DM80_3604(mhbM)

BMUL:

NP80_4175(mhbM)

BCT:

GEM_4469

BCED:

DM42_3818(mhbM)

BCEP:

APZ15_18310

BCON:

NL30_06230

BDF:	WI26_19460

BTEI:

WS51_04055

BPSL:

WS57_07660

BMEC:

WJ16_24850

BSTL:

BBJ41_19360

BANN:

JFN94_23565

BARI:

NLX30_32270

BAEN:

L3V59_19375

BGO:	BM43_4139(nagX)

BYI:	BYI23_C002800

BUE:	BRPE67_CCDS09420

BUZ:	AYM40_11940

PACP:

FAZ97_33175

PPAI:

E1956_27195

POLY:

PSHI2_16830

POLN:

PSHI8_16840

PPK:	U875_20465

PPNO:

DA70_13155

PPNM:

LV28_17545

PRB:	X636_13710

PPUL:

RO07_11650

PSPU:

NA29_07575

PAPI:

SG18_11980

PVE:	UC34_03450

POX:	MB84_06150 MB84_07885

PFG:	AB870_14580

PNR:	AT302_11600

PAND:

DRB87_03100

PFIB:

PI93_003080

PCOM:

NTU39_03785

PANN:

PanNE5_07720

PANX:

OYT13_02960

TVL:	FAZ95_29400

BPT:	Bpet0509 Bpet1428

BAV:

BAV1787

BHZ:	ACR54_02471(mhbM)

BPDZ:

BBN53_09845

AXY:	AXYL_05897(mhbM)

AXO:	NH44784_016421

AXN:	AX27061_0548

AXX:	ERS451415_00540(mhbM)

ADT:	APT56_02825

AIS:	BUW96_28515

ASW:	CVS48_09390

ACHR:

C2U31_07410

ACHB:

DVB37_25380

ADY:	HLG70_24140

ACHO:

H4P35_24445

AMUI:

PE062_21170

APES:

FOC84_09645

AAEG:

RA224_29350

ASEL:

RAS12_25220

ACHM:

V6V88_25035

ACHA:

KYT87_26625

AVH:	WHX56_01535

PUD:	G9Q38_03205 G9Q38_03995 G9Q38_07650

AMIM:

MIM_24p00150

AALN:

Q3V95_08080

PIG:	EGT29_07605 EGT29_26025

PARK:

LSG25_16430

NARM:

N7E01_08155

RHF:	EUB48_11995

RLH:	MIZ03_0198 MIZ03_2026

POL:

Bpro_2128

PNA:

Pnap_3144

POS:	DT070_19585

POO:	F7R28_10985

ACK:	C380_12540

DAC:

Daci_3703

DEL:	DelCs14_3111

DTS:	BI380_04665

DHK:	BO996_16830

DLA:	I6G47_05610

VAP:	Vapar_0355

VPE:	Varpa_0370 Varpa_5098

VPD:	VAPA_1c04210

VBO:	CKY39_02010

VAM:	C4F17_17010

VAZ:	H7F35_06805 H7F35_13960

VAV:	IG196_00800

VAE:	RZE79_01985 RZE79_33915

CRJ:	QMY55_13375

RTA:

Rta_17950

LIH:	L63ED372_01176(nagX)

HYR:	BSY239_3884(nagX)

HYC:	E5678_18615

HYN:	F9K07_18780

HCZ:	G9Q37_16565

HYY:	JNX00_21650

HYDR:

SOM08_15930 SOM08_21785

DIH:	G7047_06800

STHM:

IS481_16040

PBH:	AAW51_2010

SNN:	EWH46_00500

LCH:

Lcho_3669

JSV:	CNX70_17175

JAS:	FJQ89_06285

JRI:	P9875_17485

HEW:	HBDW_00290

GLC:	JQN73_16915

EBA:	ebA1378(s5h)

ABRE:

pbN1_11500(nagX)

AZO:	azo2422(nahG)

AOA:

dqs_2566

AZI:	AzCIB_1240

AZD:	CDA09_08480

AZR:	CJ010_15165

TCL:

Tchl_1075

TAK:	Tharo_1113

ACOM:

CEW83_02845

MES:

Meso_0052

OCH:	CES85_5237(xlnD)

BJU:	BJ6T_69760

BRK:	CWS35_03695 CWS35_17225

BARH:

WN72_15305

BEL:	BE61_12850

BSEP:

HAP48_0018310 HAP48_0029900

BQB:	J4P68_0021800

BSEI:

KMZ68_24835

BAUT:

QA635_40125

BBRA:

QA636_41180

BGK:	IC762_03740

BPAH:

QA639_40155

BRAZ:

LRP30_11855

XDI:	EZH22_12775

LNE:	FZC33_07110

RHZ:	RHPLAN_00130 RHPLAN_07540 RHPLAN_20830 RHPLAN_58370

MAAD:

AZF01_02545

MLUT:

JET14_03595

PSIN:

CAK95_01775 CAK95_16830 CAK95_22270

RBM:

TEF_07880

SIL:

SPO3692

JAN:

Jann_2908

PGD:

Gal_04153

CID:

P73_1457

CEH:	CEW89_18625

CBAK:

DA792_03665

CON:	TQ29_16425

PABY:

Ga0080574_TMP3110

AHT:	ANTHELSMS3_02406 ANTHELSMS3_04577

THAA:

CFI11_22050

MALU:

KU6B_12340

TRY:	QF118_04220

PAEZ:

PAE61_01700

MALC:

QQG91_01965

CBOT:

ATE48_17610

RAP:	RHOA_2120(nagX)

SRF:	LHU95_07660

ABQ:	ABAZ39_29180

ATI:	AL072_21230

AOZ:	HUE56_08620

AARE:

D3093_30860

AZP:	E6C67_12765

FER:	FNB15_01470

SNEA:

NBZ79_04380

BACO:

OXB_0838

BEO:

BEH_11950

PARH:

I5S86_00540

BKW:	BkAM31D_03935(mhbM) BkAM31D_23545(xlnD_2)

BKO:	CKF48_08415

LYG:	C1N55_03805

LMAC:

I6G82_23700

FAR:	ABE41_018090

ACOP:

RI196_06210

BSJ:	UP17_05920

BMUR:

ABE28_008485

PBUT:

DTO10_18640

PASA:

BAOM_1863

PFRI:

L8956_06455

PERI:

RE409_06820

NCM:	QNK12_04440

MSEM:

GMB29_01110

GYC:	GYMC61_2253

GYA:	GYMC52_1381

GJF:	M493_07430

SEQO:

SE1039_23060

STAP:

AOB58_2095

BRUM:

NDK47_19540

PNP:	IJ22_26140 IJ22_51690

ADAU:

NZD86_15295

AFAS:

NZD89_07200

FFE:	LSG31_15395

SIV:

SSIL_3714

SSIL:

SOLI23_19490

PSHE:

QWY16_02960

SAY:

TPY_1281

MSA:	Mycsm_01819

MDX:	BTO20_29025

MLL:	B1R94_28765

MAB:

MAB_0288

MMV:	MYCMA_0154

MABB:

MASS_0290

MABL:

MMASJCM_0287

MCHE:

BB28_01425

MIZ:	BAB75_01975

MSAO:

MYCSP_01255

MSAL:

DSM43276_00244(xlnD_1)

CGL:	Cgl3026(Cgl3026)

CGB:

cg3354

CGU:

WA5_2923

CGT:

cgR_2913

CGS:	C624_14855

CGG:	C629_14865

CGM:

cgp_3354

CGJ:

AR0_14655

CGQ:	CGLAR1_14510

CGX:	SB89_14510

CEF:

CE2863

CHN:	A605_04735

CCN:	H924_12685

CMD:	B841_04345

CGY:	CGLY_10980

CHM:	B842_04175

CMQ:	B840_03695

CDX:	CDES_13725

CCJZ:

ccrud_13660

CEE:	CENDO_03450

CRL:	NCTC7448_01565

CSUR:

N24_3116

CCYS:

SAMEA4530656_1549

CYZ:	C3B44_07750

CCOE:

CETAM_04075

COK:	COCCU_04360

CPOY:

GP475_11580

CFAC:

CFAEC_13330

CGF:	CGUA_04370

CSUE:

QP029_08245

CBRE:

QP027_03530

BFV:	C628_15275

NFR:	ERS450000_00740

NTP:	CRH09_24065

NTC:	KHQ06_24185

RHA:	RHA1_ro01869

ROP:

ROP_15470

ROA:	Pd630_LPD06032

RAV:	AAT18_09000

RRZ:	CS378_23960

RRT:	4535765_00318(xlnD) 4535765_01432

RCR:	NCTC10994_01367

RKO:	JWS14_09565

RGO:	KYT97_18770

RPSK:

JWS13_36270

RGOR:

NMQ04_06380

RHOU:

KZJ41_10125

RHOW:

A4U64_15595

RHOJ:

JVH1_27945 JVH1_38195

RHOS:

QWW67_26905

RHOQ:

QWW98_26930

RHOY:

QNA09_06620

GRU:	GCWB2_11220(mhbM)

GOI:	LK459_13275

TSD:	MTP03_03860

SALB:

XNR_0599

SGRF:

SGFS_023500

SCYE:

R2B67_04390

SCB:	SCAB_12141

SSX:	SACTE_1745

SDV:	BN159_7509

SGU:	SGLAU_17245(xlnD1) SGLAU_17380(xlnD2)

STRM:

M444_18400

SAMB:

SAM23877_1103(mhbM)

SRW:	TUE45_05046(xlnD)

SGM:	GCM10017557_24310

SCAD:

DN051_13645 DN051_36950 DN051_38295

SPLU:

LK06_026300

STRD:

NI25_24455

SALJ:

SMD11_3540

SDX:	C4B68_10315

SNQ:	CP978_25590

SGZ:	C0216_00455 C0216_00555

SVN:	CP980_04345

SNK:	CP967_07105

SRK:	FGW37_15810

SVR:	CP971_31795

SBY:	H7H31_00740

SPHV:

F9278_00565

SFUG:

CNQ36_01980

SBAT:

G4Z16_01205

SSPB:

CP982_28340

SFEU:

IM697_28935

SVD:	CP969_28475

SPRA:

CP972_27270 CP972_27995 CP972_28100

SROI:

IAG44_34530

SGJ:	IAG43_24335

SLF:	JEQ17_47565

SDEC:

L3078_43675

SAKB:

K1J60_09320

SNIG:

HEK616_55530

SCIR:

STRCI_000981

SENG:

OJ254_01920

SGK:	PET44_17155

SLAI:

P8A22_08160

SINN:

ABB07_37120

STRZ:

OYE22_00840

SCAY:

PYS65_08975

SCHE:

QRN89_26485

STII:

DRB96_19750

STIC:

DRB89_19840

KSK:

KSE_58270

KIT:	CFP65_3596

KCH:	O1G21_09990 O1G21_27125 O1G21_36210

STRI:

C7M71_014685

ABRY:

NYE86_21155

AREV:

RVR_7875

SALC:

C2138_00735

SALD:

FVA74_01535 FVA74_01685

HEA:	HL652_17985

ART:

Arth_2055

ARE:	AL755_09505

ARW:	MB46_06810

ARH:	AHiyo8_19200

ARU:	ASPU41_15325

ARQ:	BWQ92_22040

ARN:	CGK93_01920

ARX:	ARZXY2_3573

ARTP:

E5206_13300

ASUN:

KG104_03590

AOD:	Q8Z05_18225

ARTU:

E7Y32_14365

ARTZ:

FCN77_12545

AAU:

AAur_0334

PAYJ:

HMI59_19595

PAGO:

IRJ34_01535

ARZ:	AUT26_01460

ACH:

Achl_0762

PSNI:

NIBR502771_01555

PSEY:

GU243_04105

PDEL:

JCQ34_03030

POK:	SMD14_03400

PSLS:

KTR40_03290

PSFE:

NG819_00435

KRS:	EQG70_12080

CFI:

Celf_3007

ICA:	Intca_3430

GYU:	FE374_02670

NMAR:

HPC71_10495

ACTQ:

OG417_33205

NOA:	BKM31_03435

NCX:	Nocox_28390

NGN:	LCN96_38575

NFS:	OIE67_21540

MHAI:

OHB01_32295

FRE:	Franean1_3612

SVI:	Svir_20710

AMQ:	AMETH_4245

AMYC:

CU254_18630

AACD:

LWP59_31245

ATHM:

L1857_24105

ACYN:

ORV05_11990

AMYD:

K1T34_05590 K1T34_09375 K1T34_38445

PBRO:

HOP40_17610 HOP40_21870 HOP40_26515

PPEL:

H6H00_21040

PSDS:

K1T35_20785

APRE:

CNX65_15965

SSYI:

EKG83_21250

KUT:	JJ691_21760(xlnD)

KPHY:

AOZ06_25110

LED:	BBK82_23365

PMAD:

BAY61_14405

ACTY:

OG774_23460

UME:	RM788_10275

MICB:

MicB006_2939

MCHL:

PVK74_09650

AIH:	Aiant_14280

DFU:	Dfulv_23930

DACT:

KZZ52_43820

AEY:	CDG81_16840

VAB:

WPS_24180

» show all

Reference

1 [PMID:4357436]

Authors

Groseclose EE, Ribbons DW, Hughes H.

Title

3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa.

Journal

Biochem Biophys Res Commun 55:897-903 (1973)
DOI:10.1016/0006-291X(73)91228-X

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.24

IUBMB Enzyme Nomenclature:

1.14.13.24

ExPASy - ENZYME nomenclature database:

1.14.13.24

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.13.24

BRENDA, the Enzyme Database:

1.14.13.24

CAS:	51570-26-4

DBGET integrated database retrieval system