KEGG ENZYME: 1.14.13.24

ENZYME: 1.14.13.24

Entry

EC 1.14.13.24 Enzyme

Name

3-hydroxybenzoate 6-monooxygenase;
3-hydroxybenzoate 6-hydroxylase;
m-hydroxybenzoate 6-hydroxylase;
3-hydroxybenzoic acid-6-hydroxylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

3-hydroxybenzoate,NADH:oxygen oxidoreductase (6-hydroxylating)

Reaction(IUBMB)

3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O [RN:R02589]

Reaction(KEGG)

R02589

Substrate

3-hydroxybenzoate [CPD:C00587];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

2,5-dihydroxybenzoate [CPD:C00628];
NAD+ [CPD:C00003];
H2O [CPD:C00001]

Comment

A flavoprotein (FAD). Acts also on a number of analogues of 3-hydroxybenzoate substituted in the 2, 4, 5 and 6 positions; NADPH can act instead of NADH, but more slowly.

History

EC 1.14.13.24 created 1984

Pathway

ec00362

Benzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K22270

3-hydroxybenzoate 6-monooxygenase

Genes

ECE:

Z3390

ECS:

ECs_3027

ECF:	ECH74115_3267

ETW:

ECSP_3013

ELX:	CDCO157_2790

ECOO:

ECRM13514_5372

ECOH:

ECRM13516_3724

EOK:	G2583_2678

ELR:	ECO55CA74_13240

EAL:	EAKF1_ch3857

STY:

STY2405

STT:

t0680

SEX:	STBHUCCB_7280

SENT:

TY21A_03470

STM:

STM2175

SEO:	STM14_2682

SEV:	STMMW_22071

SEY:	SL1344_2152

SEM:	STMDT12_C21960

SEJ:	STMUK_2205

SEB:	STM474_2264

SEF:	UMN798_2349

SETU:

SETC:

SENR:

SEND:

SENI:

SEEN:

SPT:

SEK:

SPQ:	SPAB_00841

SEI:

SPC_1527

SEC:	SCH_2192(nhg)

SEH:	SeHA_C2410

SHB:

SU5_02767

SENH:

CFSAN002069_20950

SEEH:

SEEH1578_20150

SEE:	SNSL254_A2365

SENN:

SN31241_32870

SEW:	SeSA_A2414

SEA:	SeAg_B2321

SENS:

SED:

SEG:

SEL:

SEGA:

SET:

SENA:

SENO:

SENV:

SENQ:

SENL:

SENJ:

SEEC:

SEEB:

SEEP:

SENB:

SENE:

SENC:

SBG:

SBZ:

SBV:	N643_09685

SALZ:

EOS98_08090

KOX:

KOX_25675

KOE:

A225_4035

KOM:	HR38_23810

KOK:	KONIH1_19970

KOC:	AB185_16310

EAE:

EAE_23875

EAR:

CCG29101

KQV:	B8P98_08965

KLL:	BJF97_19860

KLW:	DA718_09145

KAR:	LGL98_07905

RON:	TE10_19725

RTG:	NCTC13098_02137(mhbM)

CRO:

ROD_22631

CKO:

CKO_00654

CFD:	CFNIH1_21965

CBRA:

A6J81_00350

CYO:	CD187_08425

CPOT:

FOB25_19515

CFQ:	C2U38_16180

CAMA:

F384_11420

CAF:	AL524_11385

CFAR:

CI104_16805

CIR:	C2U53_26655

CIE:	AN232_13145

CTEL:

GBC03_13665

CARS:

E1B03_17625

EBT:	EBL_c13690

PGE:	LG71_12560

KAS:	KATP_15130

PSGC:

G163CM_04730(mhbM)

EBF:

D782_1486

SMAR:

SM39_2826(mhbM)

SMAC:

SMDB11_2639(mhbM)

XBV:	XBW1_2121(mhbM)

PRAG:

EKN56_19220

XHY:	FZ025_09535

PEN:	PSEEN2596(mhbM)

PCZ:	PCL1606_26180

PSEM:

TO66_18335

ALC:	OTEC02_09095

ASEI:

I8T81_09000

ACD:	AOLE_09105

AEI:	AOY20_07145

AWG:	GFH30_03355

HAK:	KO116_03925

MARS:

A8C75_13630

ASIM:

FE240_04935

OCE:

GU3_03005

ZDF:	AN401_12515

NAQ:	D0T90_04540

VFF:	VITFI_CDS3374

VIT:	ADP71_15240

PSE:

NH8B_2047

RPJ:	N234_25225 N234_25345

REH:	H16_B0876(h16_B0876)

CNC:	CNE_2c08340(mhbM) CNE_BB2p00890(xlnD)

CUH:	BJN34_30895

REU:	Reut_B5805 Reut_B5861

CBW:	RR42_s0703 RR42_s2029

CCUP:

BKK81_09165

CUP:	BKK80_09450

CUU:	BKK79_03670

COX:	E0W60_03835 E0W60_09680

BUR:	Bcep18194_B1886

BCN:

Bcen_4218

BCH:	Bcen2424_4148

BCM:	Bcenmc03_3371

BCJ:	BCAM1274(mhbM)

BCEN:

DM39_3727(mhbM)

BCEO:

I35_5123(mhbM)

BMJ:	BMULJ_04070(nahG)

BMU:

Bmul_4439

BMK:	DM80_3604(mhbM)

BMUL:

NP80_4175(mhbM)

BCT:

GEM_4469

BCED:

DM42_3818(mhbM)

BCEP:

APZ15_18310

BCON:

NL30_06230

BDF:	WI26_19460

BTEI:

BPSL:

BMEC:

BSTL:

BANN:

BGO:	BM43_4139(nagX)

BYI:	BYI23_C002800

BUE:	BRPE67_CCDS09420

BUZ:	AYM40_11940

PACP:

FAZ97_33175

PPK:	U875_20465

PPNO:

DA70_13155

PPNM:

LV28_17545

PRB:	X636_13710

PPUL:

RO07_11650

PSPU:

NA29_07575

PAPI:

SG18_11980

PVE:	UC34_03450

POX:	MB84_06150 MB84_07885

PFG:	AB870_14580

PNR:	AT302_11600

PAND:

DRB87_03100

PFIB:

PI93_003080

BPT:	Bpet0509 Bpet1428

BAV:

BAV1787

BHZ:	ACR54_02471(mhbM)

BPDZ:

BBN53_09845

AXY:	AXYL_05897(mhbM)

AXO:	NH44784_016421

AXN:	AX27061_0548

AXX:	ERS451415_00540(mhbM)

ADT:	APT56_02825

AIS:	BUW96_28515

ASW:	CVS48_09390

ACHR:

C2U31_07410

ACHB:

DVB37_25380

ADY:	HLG70_24140

PUD:	G9Q38_03205 G9Q38_03995 G9Q38_07650

AMIM:

MIM_24p00150

PIG:	EGT29_07605 EGT29_26025

PARK:

LSG25_16430

RHF:	EUB48_11995

POL:

Bpro_2128

PNA:

Pnap_3144

POS:	DT070_19585

POO:	F7R28_10985

PVAC:

HC248_01019(nagX)

ACK:	C380_12540

DAC:

Daci_3703

DEL:	DelCs14_3111

DTS:	BI380_04665

DHK:	BO996_16830

DLA:	I6G47_05610

VAP:	Vapar_0355

VPE:	Varpa_0370 Varpa_5098

VPD:	VAPA_1c04210

VBO:	CKY39_02010

VAM:	C4F17_17010

RTA:

Rta_17950

LIH:	L63ED372_01176(nagX)

HYR:	BSY239_3884(nagX)

HYC:	E5678_18615

HYN:	F9K07_18780

DIH:	G7047_06800

MJE:	LVC68_12750

STHM:

IS481_16040

JSV:	CNX70_17175

JAS:	FJQ89_06285

GLC:	JQN73_16915

LCH:

Lcho_3669

SNN:	EWH46_00500

PBH:	AAW51_2010

EBA:	ebA1378(s5h)

ABRE:

pbN1_11500(nagX)

AZO:	azo2422(nahG)

AOA:

dqs_2566

AZI:	AzCIB_1240

ACOM:

CEW83_02845

AZD:	CDA09_08480

AZR:	CJ010_15165

TCL:

Tchl_1075

TAK:	Tharo_1113

MES:

Meso_0052

OCH:	CES85_5237(xlnD)

BJU:	BJ6T_69760

BRK:	CWS35_03695 CWS35_17225

BARH:

WN72_15305

BEL:	BE61_12850

XDI:	EZH22_12775

LNE:	FZC33_07110

RHZ:	RHPLAN_00130 RHPLAN_07540 RHPLAN_20830 RHPLAN_58370

MAAD:

AZF01_02545

MLUT:

JET14_03595

PSIN:

CAK95_01775 CAK95_16830 CAK95_22270

RBM:

SIL:

JAN:

PGD:

CID:

CEH:	CEW89_18625

CON:	TQ29_16425

PABY:

Ga0080574_TMP3110

AHT:	ANTHELSMS3_02406 ANTHELSMS3_04577

THAA:

CFI11_22050

MALU:

KU6B_12340

CBOT:

ATE48_17610

ABQ:	ABAZ39_29180

ATI:	AL072_21230

AOZ:	HUE56_08620

FER:	FNB15_01470

BACO:

OXB_0838

BEO:

BEH_11950

PARH:

I5S86_00540

BKW:	BkAM31D_03935(mhbM) BkAM31D_23545(xlnD_2)

BKO:	CKF48_08415

GYC:	GYMC61_2253

GYA:	GYMC52_1381

GJF:	M493_07430

LYG:	C1N55_03805

FAR:	ABE41_018090

BSJ:	UP17_05920

BMUR:

PBUT:

PASA:

MSEM:

SEQO:

PNP:	IJ22_26140 IJ22_51690

SIV:

SSIL_3714

SSIL:

SOLI23_19490

SAY:

TPY_1281

MSA:	Mycsm_01819

MDX:	BTO20_29025

MLL:	B1R94_28765

MAB:

MAB_0288

MMV:	MYCMA_0154

MABB:

MASS_0290

MABL:

MMASJCM_0287

MCHE:

BB28_01425

MIZ:	BAB75_01975

MSAO:

MYCSP_01255

MSAL:

DSM43276_00244(xlnD_1)

CGL:	Cgl3026(Cgl3026)

CGB:

cg3354

CGU:

WA5_2923

CGT:

cgR_2913

CGS:	C624_14855

CGG:	C629_14865

CGM:

cgp_3354

CGJ:

AR0_14655

CGQ:	CGLAR1_14510

CGX:	SB89_14510

CEF:

CE2863

CHN:	A605_04735

CCN:	H924_12685

CMD:	B841_04345

CGY:	CGLY_10980

CHM:	B842_04175

CMQ:	B840_03695

CDX:	CDES_13725

CCJZ:

ccrud_13660

CEE:	CENDO_03450

CRL:	NCTC7448_01565

CSUR:

N24_3116

CCYS:

SAMEA4530656_1549

CYZ:	C3B44_07750

BFV:	C628_15275

NFR:	ERS450000_00740

NTP:	CRH09_24065

NTC:	KHQ06_24185

RHA:	RHA1_ro01869

ROP:

ROP_15470

ROA:	Pd630_LPD06032

RAV:	AAT18_09000

RRZ:	CS378_23960

RRT:	4535765_00318(xlnD) 4535765_01432

RCR:	NCTC10994_01367

RKO:	JWS14_09565

RGO:	KYT97_18770

GRU:	GCWB2_11220(mhbM)

SALB:

XNR_0599

SCB:	SCAB_12141

SSX:	SACTE_1745

SDV:	BN159_7509

SGU:	SGLAU_17245(xlnD1) SGLAU_17380(xlnD2)

STRM:

M444_18400

SAMB:

SAM23877_1103(mhbM)

SRW:	TUE45_05046(xlnD)

SGM:	GCM10017557_24310

SCAD:

DN051_13645 DN051_36950 DN051_38295

SPLU:

LK06_026300

STRD:

NI25_24455

SALJ:

SMD11_3540

SDX:	C4B68_10315

SNQ:	CP978_25590

SGZ:	C0216_00455 C0216_00555

SVN:	CP980_04345

SNK:	CP967_07105

SRK:	FGW37_15810

SVR:	CP971_31795

SBY:	H7H31_00740

SPHV:

SFUG:

SBAT:

SSPB:

SFEU:

SVD:	CP969_28475

SPRA:

CP972_27270 CP972_27995 CP972_28100

KSK:

KSE_58270

KIT:	CFP65_3596

STRI:

C7M71_014685

SALC:

C2138_00735

SALD:

FVA74_01535 FVA74_01685

HEA:	HL652_17985

ART:

Arth_2055

ARE:	AL755_09505

ARW:	MB46_06810

ARH:	AHiyo8_19200

ARZ:	AUT26_01460

ARU:	ASPU41_15325

ARQ:	BWQ92_22040

ARN:	CGK93_01920

ARX:	ARZXY2_3573

ARTP:

AAU:

ACH:

PSNI:

PSEY:

KRS:	EQG70_12080

CFI:

Celf_3007

ICA:	Intca_3430

GYU:	FE374_02670

NOA:	BKM31_03435

NCX:	Nocox_28390

NGN:	LCN96_38575

FRE:	Franean1_3612

SVI:	Svir_20710

AMQ:	AMETH_4245

AMYC:

CU254_18630

PBRO:

HOP40_17610 HOP40_21870 HOP40_26515

APRE:

CNX65_15965

SSYI:

EKG83_21250

KPHY:

AOZ06_25110

LED:	BBK82_23365

PMAD:

BAY61_14405

MICB:

MicB006_2939

AIH:	Aiant_14280

AEY:	CDG81_16840

» show all

Reference

1 [PMID:4357436]

Authors

Groseclose EE, Ribbons DW, Hughes H.

Title

3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa.

Journal

Biochem Biophys Res Commun 55:897-903 (1973)
DOI:10.1016/0006-291X(73)91228-X

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.24

IUBMB Enzyme Nomenclature:

1.14.13.24

ExPASy - ENZYME nomenclature database:

1.14.13.24

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.13.24

BRENDA, the Enzyme Database:

1.14.13.24

CAS:	51570-26-4

DBGET integrated database retrieval system