EC              Enzyme                                 

phenol 2-monooxygenase (NADH);
dmpLMNOP (gene names)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
phenol,NADH:oxygen oxidoreductase (2-hydroxylating)
phenol + NADH + H+ + O2 = catechol + NAD+ + H2O [RN:R10043]
(other) R10042
phenol [CPD:C00146];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
catechol [CPD:C00090];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
The enzyme, characterized from the bacteria Pseudomonas sp. CF600 and Acinetobacter radioresistens, consists of a multisubunit oxygenease component that contains the active site and a dinuclear iron center, a reductase component that contains FAD and one iron-sulfur cluster, and a regulatory component. The reductase component is responsible for transferring electrons from NADH to the dinuclear iron center.
EC created 2019
ec00361  Chlorocyclohexane and chlorobenzene degradation
ec00362  Benzoate degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
K16242  phenol/toluene 2-monooxygenase (NADH) P3/A3
K16243  phenol/toluene 2-monooxygenase (NADH) P1/A1
K16244  phenol/toluene 2-monooxygenase (NADH) P2/A2
K16245  phenol/toluene 2-monooxygenase (NADH) P4/A4
K16246  phenol/toluene 2-monooxygenase (NADH) P5/A5
PSD: DSC_04415 DSC_04420 DSC_04425 DSC_04430 DSC_04435
RBD: ALSL_0896 ALSL_0897 ALSL_0898 ALSL_0899 ALSL_0900
PRE: PCA10_06970(dmpL) PCA10_06980(dmpM) PCA10_06990(dmpN) PCA10_07000(dmpO) PCA10_07010(dmpP)
PFUW: KF707C_6700(dmpL) KF707C_6710(dmpM) KF707C_6720(dmpN) KF707C_6730(dmpO) KF707C_6740(dmpP)
PCQ: PcP3B5_25310(mphL) PcP3B5_25320(dmpM) PcP3B5_25330(tmoA) PcP3B5_25340 PcP3B5_25350(dmpP)
PPG: PputGB1_3306 PputGB1_3307 PputGB1_3308 PputGB1_3309 PputGB1_3310
PPJ: RK21_04242 RK21_04243 RK21_04244 RK21_04245 RK21_04246 RK21_04247
PVR: PverR02_14660 PverR02_14665 PverR02_14670 PverR02_14675 PverR02_14680
PKC: PKB_1742(dmpL) PKB_1743 PKB_1744(dmpN) PKB_1745 PKB_1746(dmpP)
PALK: PSAKL28_05880 PSAKL28_05890 PSAKL28_05900 PSAKL28_05910 PSAKL28_05920
AVN: Avin_08810(dmpP) Avin_08820(dmpO) Avin_08830(dmpN) Avin_08840(dmpM) Avin_08850(dpmL) Avin_30710(lapP) Avin_30720(lapO) Avin_30730(lapN) Avin_30740(lapM) Avin_30750(lapL)
AVL: AvCA_08810(dmpP) AvCA_08820(dmpO) AvCA_08830(dmpN) AvCA_08840(dmpM) AvCA_08850(dpmL) AvCA_30710(lapP) AvCA_30720(lapO) AvCA_30730(lapN) AvCA_30740(lapM) AvCA_30750(lapL)
AVD: AvCA6_08810(dmpP) AvCA6_08820(dmpO) AvCA6_08830(dmpN) AvCA6_08840(dmpM) AvCA6_08850(dpmL) AvCA6_30710(lapP) AvCA6_30720(lapO) AvCA6_30730(lapN) AvCA6_30740(lapM) AvCA6_30750(lapL)
MARJ: MARI_05340(mphL) MARI_05350(dmpM) MARI_05360(tmoA) MARI_05370 MARI_05380(dmpP)
ACC: BDGL_000470(mphP) BDGL_000471(mphO) BDGL_000472(mphN) BDGL_000473(mphM) BDGL_000474(mphL)
AGU: AS4_16410(dsoF) AS4_16420(dsoE) AS4_16430(dsoD) AS4_16440(dsoC) AS4_16450(dsoB)
NJP: NEJAP_0663(dmpL) NEJAP_0664(dmpM) NEJAP_0665(dmpN) NEJAP_0666(dmpO) NEJAP_0667(dmpP)
AJP: AMJAP_3006(dmpP) AMJAP_3007(dmpO) AMJAP_3008(dmpN) AMJAP_3009(dmpM) AMJAP_3010(dmpL)
REH: H16_B0540(poxB) H16_B0541(poxC) H16_B0542(poxD) H16_B0543(poxE) H16_B0544(poxF)
CNC: CNE_2c04860(poxB) CNE_2c04870(poxC) CNE_2c04880(poxD) CNE_2c04890(poxE) CNE_2c04900(poxF)
RME: Rmet_1326(tomA5) Rmet_1327(tomA4) Rmet_1328(tomA3) Rmet_1329(tomA2) Rmet_1330(tomA1) Rmet_1782(phyE) Rmet_1783(phyD) Rmet_1784(phyC) Rmet_1785(phyB) Rmet_1786(phyA)
BCEN: DM39_4839(dmpP) DM39_4840(phO) DM39_4841(phN) DM39_4842(dmpM) DM39_4843(mphL)
BCED: DM42_4998(dmpP) DM42_4999(phO) DM42_5000(phN) DM42_5001(dmpM) DM42_5002(phL)
HPSE: HPF_01905(dmpP) HPF_01910(tbuD) HPF_01915(tmoA2) HPF_01920(dmpM) HPF_01925(mphL)
MPT: Mpe_A2280(dmpP) Mpe_A2281(dmpO) Mpe_A2282(dmpN) Mpe_A2283(dmpM) Mpe_A2284(dmpL) Mpe_A3305(dmpO) Mpe_A3306(dmpN) Mpe_A3307(dmpM) Mpe_A3308(dmpL)
AZO: azo1846(lapL) azo1847(lapM) azo1848(lapN) azo1849(lapO) azo1850(lapP) azo2439(poxF) azo2440(poxE) azo2441(poxD) azo2442(poxC) azo2443(poxB)
ACIB: ACBT_0275(dmpP) ACBT_0276(dmpO) ACBT_0277(dmpN) ACBT_0278(dmpM) ACBT_0279(dmpL)
AFC: AFAEC_1853(dmpP) AFAEC_1854(dmpO) AFAEC_1855(dmpN) AFAEC_1856(dmpM) AFAEC_1857(dmpL)
AAQI: AAQM_0970(dmpL) AAQM_0971(dmpM) AAQM_0972(dmpN) AAQM_0973(dmpO) AAQM_0974(dmpP)
ACLO: ACLO_a0150(dmpP) ACLO_a0151(dmpO) ACLO_a0152(dmpN) ACLO_a0153(dmpM) ACLO_a0154(dmpL)
ADZ: ADFLV_2564(dmpP) ADFLV_2565(dmpO) ADFLV_2566(dmpN) ADFLV_2567(dmpM) ADFLV_2568(dmpL)
ALK: ALEK_0371(dmpL) ALEK_0372(dmpM) ALEK_0373(dmpN) ALEK_0374(dmpO) ALEK_0375(dmpP)
AANA: AANAER_1246(dmpL) AANAER_1247(dmpM) AANAER_1248(dmpN) AANAER_1249(dmpO) AANAER_1250(dmpP)
SJP: SJA_C1-11990(tomA1) SJA_C1-12000(tomA2) SJA_C1-12010(tomA3) SJA_C1-12020(tomA4) SJA_C1-12030(tomA5)
ASOC: CB4_02441(mphL) CB4_02442(dmpM) CB4_02443(tmoA)
MMAG: MMAD_31790 MMAD_31810(mphN)
MHIB: MHIB_39890(mphP)
 » show all
1  [PMID:2254258]
Nordlund I, Powlowski J, Shingler V
Complete nucleotide sequence and polypeptide analysis of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600.
J Bacteriol 172:6826-33 (1990)
2  [PMID:2254259]
Powlowski J, Shingler V
In vitro analysis of polypeptide requirements of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600.
J Bacteriol 172:6834-40 (1990)
3  [PMID:8995386]
Powlowski J, Sealy J, Shingler V, Cadieux E
On the role of DmpK, an auxiliary protein associated with multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600.
J Biol Chem 272:945-51 (1997)
4  [PMID:9012665]
Qian H, Edlund U, Powlowski J, Shingler V, Sethson I
Solution structure of phenol hydroxylase protein component P2 determined by NMR spectroscopy.
Biochemistry 36:495-504 (1997)
5  [PMID:12186554]
Cadieux E, Vrajmasu V, Achim C, Powlowski J, Munck E
Biochemical, Mossbauer, and EPR studies of the diiron cluster of phenol hydroxylase from Pseudomonas sp. strain CF 600.
Biochemistry 41:10680-91 (2002)
Other DBs
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