Entry
Name
L-aspartate N-monooxygenase (nitrosuccinate-forming);
creE (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
L-aspartate,NADPH:oxygen oxidoreductase [(2S)-2-nitrobutanedioate-forming]
Reaction(IUBMB)
L-aspartate + 3 NADPH + 3 H+ + 3 O2 = (2S)- 2-nitrobutanedioate + 3 NADP+ + 4 H2O [RN:
R12907 ];
(1a) L-aspartate + NADPH + H+ + O2 = N-hydroxy-L-aspartate + NADP+ + H2O [RN:
R12908 ];
(1b) N-hydroxy-L-aspartate + NADPH + H+ + O2 = N,N-dihydroxy-L-aspartate + NADP+ + H2O [RN:
R12909 ];
(1c) N,N-dihydroxy-L-aspartate = (2S)-2-nitrosobutanedioate + H2O (spontaneous) [RN:
R12910 ];
(1d) (2S)-2-nitrosobutanedioate + NADPH + H+ + O2 = (2S)-2-nitrobutanedioate + NADP+ + H2O [RN:
R12911 ]
Reaction(KEGG)
Substrate
Product
(2S)-2-nitrosobutanedioate [CPD:
C22334 ];
NADP+ [CPD:
C00006 ];
H2O [CPD:
C00001 ];
N-hydroxy-L-aspartate [CPD:
C22380 ];
N,N-dihydroxy-L-aspartate [CPD:
C22333 ];
(2S)-2-nitrobutanedioate [CPD:
C22388 ]
Comment
The enzyme, found in some Actinobacteria, is involved in a pathway that forms nitrite, which is subsequently used to generate a diazo group in some secondary metabolites. Requires an FAD cofactor.
History
EC 1.14.13.248 created 2021
Pathway
ec00998 Biosynthesis of various antibiotics
ec01110 Biosynthesis of secondary metabolites
Orthology
K25562 L-aspartate N-monooxygenase (nitrosuccinate-forming)
Genes
SGRF : SGFS_080920 SGFS_087150
SCB : SCAB_64551 SCAB_69781
SMAL : SMALA_1625 SMALA_2310
SSOI : I1A49_11265 I1A49_14680
SDV : BN159_4422 BN159_6016 BN159_7957
STRE : GZL_06149 GZL_07531
SGM : GCM10017557_24320 GCM10017557_58080
SCAD : DN051_26665 DN051_33885
SNR : SNOUR_28525 SNOUR_34625
SPLU : LK06_008675 LK06_026295 LK06_027145 LK06_029040
SAUO : BV401_11590 BV401_14965
SALJ : SMD11_1717 SMD11_3243
SQZ : FQU76_00325 FQU76_11655
SKA : CP970_07035 CP970_30420
SPAD : DVK44_07755 DVK44_34375
SBY : H7H31_09700 H7H31_25340
SSPB : CP982_00270 CP982_13740 CP982_28265
SFEU : IM697_09865 IM697_30750
SROI : IAG44_28380 IAG44_34520
SLF : JEQ17_24415 JEQ17_32250
SDEC : L3078_11355 L3078_13925
SAKB : K1J60_31690 K1J60_34185
SCIR : STRCI_002593 STRCI_003009 STRCI_004222
SXT : KPP03845_106227 KPP03845_300046
STUD : STRTU_000145 STRTU_004997
SYUN : MOV08_28615 MOV08_34910
STRZ : OYE22_09335 OYE22_24635
SPRF : K4G22_13860 K4G22_20355
SCAJ : O1G22_29235 O1G22_43455
STRH : GXP74_26075 GXP74_26520 GXP74_28305
SSYI : EKG83_10135 EKG83_27695
KPHY : AOZ06_18050 AOZ06_18845 AOZ06_22515 AOZ06_26375 AOZ06_45890
UME : RM788_05840 RM788_07360 RM788_32275
» show all
Taxonomy
Reference
Authors
Sugai Y, Katsuyama Y, Ohnishi Y.
Title
A nitrous acid biosynthetic pathway for diazo group formation in bacteria.
Journal
Sequence
Reference
Authors
Hagihara R, Katsuyama Y, Sugai Y, Onaka H, Ohnishi Y.
Title
Novel desferrioxamine derivatives synthesized using the secondary metabolism-specific nitrous acid biosynthetic pathway in Streptomyces davawensis.
Journal
Sequence
Other DBs