KEGG   ENZYME: 1.14.13.28Help
Entry
EC 1.14.13.28               Enzyme                                 

Name
3,9-dihydroxypterocarpan 6a-monooxygenase;
3,9-dihydroxypterocarpan 6a-hydroxylase;
3,9-dihydroxypterocarpan 6alpha-monooxygenase (erroneous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(6aR,11aR)-3,9-dihydroxypterocarpan,NADPH:oxygen oxidoreductase (6a-hydroxylating)
Reaction(IUBMB)
(6aR,11aR)-3,9-dihydroxypterocarpan + NADPH + H+ + O2 = (6aS,11aS)-3,6a,9-trihydroxypterocarpan + NADP+ + H2O [RN:R03452]
Reaction(KEGG)
Substrate
(6aR,11aR)-3,9-dihydroxypterocarpan [CPD:C04271];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(6aS,11aS)-3,6a,9-trihydroxypterocarpan [CPD:C01263];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Possibly a heme-thiolate protein (P-450). The product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean.
History
EC 1.14.13.28 created 1989
Pathway
ec00943  Isoflavonoid biosynthesis
Orthology
K13261  3,9-dihydroxypterocarpan 6a-monooxygenase
Genes
GMX: 100776878(CYP93A1)
Taxonomy
Reference
1  [PMID:6540173]
  Authors
Hagmann ML, Heller W, Grisebach H.
  Title
Induction of phytoalexin synthesis in soybean. Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures.
  Journal
Eur J Biochem 142:127-31 (1984)
DOI:10.1111/j.1432-1033.1984.tb08259.x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.28
IUBMB Enzyme Nomenclature: 1.14.13.28
ExPASy - ENZYME nomenclature database: 1.14.13.28
BRENDA, the Enzyme Database: 1.14.13.28
CAS: 92584-16-2

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