Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

2-nitrophenol,NADPH:oxygen 2-oxidoreductase (2-hydroxylating, nitrite-forming)

2-nitrophenol + 2 NADPH + 2 H+ + O2 = catechol + nitrite + 2 NADP+ + H2O [RN:R00828]

R00828

2-nitrophenol [CPD:C01988];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Involved in the metabolism of nitro-aromatic compounds by a strain of Pseudomonas putida.

EC 1.14.13.31 created 1989

1  [PMID:3752997]

Zeyer J, Kocher HP, Timmis KN.

Influence of para-substituents on the oxidative metabolism of o-nitrophenols by Pseudomonas putida B2.

Appl Environ Microbiol 52:334-9 (1986)