KEGG ENZYME: 1.14.13.38

KEGG

ENZYME: 1.14.13.38

Entry

EC 1.14.13.38               Enzyme

Name

anhydrotetracycline 6-monooxygenase;
ATC oxygenase;
anhydrotetracycline oxygenase;
oxyS (gene name);
anhydrotetracycline monooxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

anhydrotetracycline,NADPH:oxygen oxidoreductase (6-hydroxylating)

Reaction(IUBMB)

anhydrotetracycline + NADPH + H+ + O2 = 12-dehydrotetracycline + NADP+ + H2O [RN:R04060]

Reaction(KEGG)

Substrate

anhydrotetracycline [CPD:C02811];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

12-dehydrotetracycline [CPD:C03206];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. It can also catalyse EC 1.14.13.234, 12-dehydrotetracycline 5-monooxygenase.

History

EC 1.14.13.38 created 1990, modified 2016

Pathway

Tetracycline biosynthesis

Biosynthesis of type II polyketide products

Metabolic pathways

Biosynthesis of secondary metabolites

Orthology

anhydrotetracycline 6-monooxygenase / 5a,11a-dehydrotetracycline 5-monooxygenase

Genes

SVR:	CP971_23960

CP984_02460

CP981_34445

NEH16_31700

K9S39_12950

DVA86_06945

PS467_23830

O1G22_08880

KAU:	B6264_18535

NEX:	NE857_24350

AGRA3207_005105 AGRA3207_005106

SD37_34210

HUW46_05066(otcC_3)

KUT:	JJ691_15570

N8J89_05295

L3i22_066710

Daura_37815 Daura_37840

COUCH_15960

Reference

1

Authors

Behal V, Hostalek Z, Vanek Z.

Title

Anhydrotetracycline oxygenase activity and biosynthesis of tetracyclines in streptomyces aureofaciens.

Journal

Biotechnol Lett 1:177-182 (1979)

Reference

2 [PMID:2914874]

Authors

Binnie C, Warren M, Butler MJ

Title

Cloning and heterologous expression in Streptomyces lividans of Streptomyces rimosus genes involved in oxytetracycline biosynthesis.

Journal

J Bacteriol 171:887-95 (1989)
DOI:10.1128/JB.171.2.887-895.1989

Reference

3 [PMID:3138982]

Authors

Vancurova I, Volc J, Flieger M, Neuzil J, Novotna J, Vlach J, Behal V

Title

Isolation of pure anhydrotetracycline oxygenase from Streptomyces aureofaciens.

Journal

Biochem J 253:263-7 (1988)
DOI:10.1042/bj2530263

Reference

4 [PMID:23621493]

Authors

Wang P, Bashiri G, Gao X, Sawaya MR, Tang Y

Title

Uncovering the enzymes that catalyze the final steps in oxytetracycline biosynthesis.

Journal

J Am Chem Soc 135:7138-41 (2013)
DOI:10.1021/ja403516u

Other DBs

ExplorEnz - The Enzyme Database:

IUBMB Enzyme Nomenclature:

ExPASy - ENZYME nomenclature database:

BRENDA, the Enzyme Database:

CAS:	70766-62-0

DBGET integrated database retrieval system