KEGG   ENZYME: 1.14.13.59Help
Entry
EC 1.14.13.59               Enzyme                                 

Name
L-lysine N6-monooxygenase (NADPH);
lysine N6-hydroxylase;
L-lysine 6-monooxygenase (NADPH) (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
L-lysine,NADPH:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
L-lysine + NADPH + H+ + O2 = N6-hydroxy-L-lysine + NADP+ + H2O [RN:R00448]
Reaction(KEGG)
Substrate
L-lysine [CPD:C00047];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
N6-hydroxy-L-lysine [CPD:C01028];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). The enzyme from strain EN 222 of Escherichia coli is highly specific for L-lysine; L-ornithine and L-homolysine are, for example, not substrates.
History
EC 1.14.13.59 created 1999, modified 2001, modified 2012
Pathway
ec00310  Lysine degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K03897  lysine N6-hydroxylase
Genes
EOJ: ECO26_1348(iucD)
EOI: ECO111_1296(iucD)
ECC: c3624(iucD)
ECV: APECO1_O1CoBM169(iucD)
ECM: EcSMS35_3179(iucD)
ECQ: ECED1_4902(iucD)
ECK: EC55989_4642(iucD)
ECT: ECIAI39_3426(iucD)
EOC: CE10_3449(iucD)
EUM: ECUMN_3372(iucD)
ECZ: ECS88_p0010(iucD)
ELN: NRG857_30237(iucD)
ESO: O3O_02285
ESM: O3M_22985
ESL: O3K_23070
EAB: ECABU_c32820(iucD)
ENA: ECNA114_4698(iucD)
ELC: i14_3317(iucD)
ELD: i02_3317(iucD)
ECOJ: P423_16375
ECOO: ECRM13514_1293(iucD)
ECOH: ECRM13516_1249(iucD)
ECOS: EC958_3274(iucD)
SFL: SF3718(iucD)
SFX: S4053(iucD)
SFV: SFV_3851(iucD)
SFE: SFxv_4051
SFN: SFy_5297
SFS: SFyv_5371
SFT: NCTC1_04015(iucD)
SSN: SSON_3604(iucD)
SBO: SBO_4340(iucD)
SBC: SbBS512_E4631(iucD)
ENC: ECL_03319(iucD)
EEC: EcWSU1_02922(iucD)
ECLX: LI66_14275
ECLZ: LI64_13685
CTU: Ctu_1p01070(iucD)
KPU: KP1_p315(iucD)
KPR: KPR_4894(iucD)
KPNK: BN49_pII0065(iucD)
CKO: CKO_00970
YPE: YPO0993(iucD) YPO1530(alcA)
YPK: y2640(ysuI) y3384(iucD)
YPM: YP_1419(alcA) YP_3447(iucD)
YPZ: YPZ3_0745(iucD) YPZ3_1395(alcA)
YPD: YPD4_0700(iucD) YPD4_1361(alcA)
YPX: YPD8_0700(iucD) YPD8_1587(alcA)
YPH: YPC_0799(iucD)
YPW: CH59_308 CH59_870(iucD)
YPJ: CH55_1008 CH55_3547(iucD)
YPV: BZ15_2022 BZ15_2580(iucD)
YPL: CH46_3596 CH46_4152(iucD)
YPS: YPTB1542(alcA) YPTB3266(iucD)
YPO: BZ17_3344(iucD) BZ17_973
YPQ: DJ40_3171(iucD) DJ40_670
YPU: BZ21_2546(iucD) BZ21_880
YPR: BZ20_2848(iucD) BZ20_422
YPC: BZ23_1159 BZ23_2827(iucD)
YAL: AT01_1814
YIN: CH53_2434(iucD)
SRL: SOD_c07750(iucD1) SOD_c23600(iucD2) SOD_c47380(iucD)
SPLY: Q5A_004125(iucD_1) Q5A_013040(iucD_2)
SMAF: D781_0884
SMAR: SM39_3651 SM39_4454(iucD)
SERF: L085_07285
PEC: W5S_0841
ETA: ETA_30290(dfoA)
EPY: EpC_32420(dfoA)
EPR: EPYR_03490(dfoA)
EAM: EAMY_3239(dfoA)
EAY: EAM_0360
EBI: EbC_25000(iucD)
ERJ: EJP617_07270(dfoA)
EGE: EM595_p0529(iucD)
PAM: PANA_0353(iucD) PANA_0636(alcA)
PAJ: PAJ_3508(iucD) PAJ_3771(alcA)
PVA: Pvag_pPag30340(dfoA)
PLU: plu4629
XBV: XBW1_3604
XNE: XNC1_1306
XNM: XNC2_1273
XDO: XDD1_1244
XPO: XPG1_1073(rhbE)
VAU: VANGNB10_67p040c(angU)
VFI: VF_A0164(iucD)
VSA: VSAL_I0135(iucD) VSAL_II0278(iucD)
PST: PSPTO_0211(iucD)
PFC: PflA506_2780(pmsF)
PEN: PSEEN2502(basC)
PSEC: CCOS191_2629(rhbE)
PALI: A3K91_1223
PSYC: DABAL43B_1549(rhbE)
ABY: ABAYE1094
ABC: ACICU_02580(basC)
ABN: AB57_2812
ABX: ABK1_2699
ABD: ABTW07_2829(basC)
ABH: M3Q_2847
ABAD: ABD1_16330 ABD1_23780(basC)
ACC: BDGL_001871(basC)
SON: SO_3030(pubA)
SBL: Sbal_2708
SWD: Swoo_0586
PSM: PSM_B0023
PSEO: OM33_21425
PEA: PESP_b0029(iucD)
PTU: PTUN_a4218(iucD)
PTD: PTET_b0027(iucD)
SAGA: M5M_06455
LPO: LPO_2065(iucD)
LPF: lpl1946
LHA: LHA_1351(iucD)
FPH: Fphi_0923
FPT: BZ13_1108
FPI: BF30_1152
FPM: LA56_1255
FPX: KU46_405
FPZ: LA55_1975
FPJ: LA02_330
NOC: Noc_1811
NWA: Nwat_1301
CSA: Csal_1054
HEL: HELO_2824
KKO: Kkor_2040
KGE: TQ33_0707
ADH: CK627_03410(basC)
RSO: RSc1817(iucD)
RSN: RSPO_m01615(iucD)
RSE: F504_1572
BCJ: BCAS0043
BCEO: I35_7041
BCED: DM42_6412
BPC: BPTD_2414(alcA)
BPER: BN118_2542(alcA)
BPET: B1917_1405(alcA)
BPEU: Q425_21680(alcA)
BPAR: BN117_3407(alcA)
BBM: BN115_3565(alcA)
BBH: BN112_4522(alcA)
BBX: BBS798_3674(alcA)
BHO: D560_3389
BHM: D558_3365
AXY: AXYL_03070(alcA)
AXX: ERS451415_02967(iucD)
AMIM: MIM_c25820(alcA1) MIM_c25860(alcA2)
AAV: Aave_4219
AAA: Acav_4092
AOA: dqs_2667
BBA: Bd1573
BBAT: Bdt_1566
BBAC: EP01_04085
BMX: BMS_0525(iucD)
MLO: mlr5923
SME: SMa2408(rhbE)
SMEL: SM2011_a2408(rhbE)
ARA: Arad_8229(vbsO)
AVI: Avi_5731
RET: RHE_PF00456(vbsO)
REC: RHECIAT_PC0000681(vbsO)
REL: REMIM1_PF00420(iucD)
REI: IE4771_PD00518(iucD)
REP: IE4803_PC00508(iucD)
RLE: pRL120314(vbsO)
OAN: Oant_3254
OAH: DR92_3235(iucD)
AZC: AZC_3247
MET: M446_5059
MSL: Msil_3318
HDI: HDIA_3128(iucD)
TXI: TH3_13065
BAQ: BACAU_0966(iucD)
BAMP: B938_04825
BAMA: RBAU_0968
BAMN: BASU_0945(rhbE)
BAMB: BAPNAU_2795(hxyA)
BAMT: AJ82_05650
BAMY: V529_09450
BMP: NG74_01015(iucD)
BAO: BAMF_1075(RBAM_010080)
BQL: LL3_01069
BQY: MUS_1025(rhbE)
BAMI: KSO_014840
BAMC: U471_09950
BAMF: U722_05090
BATR: TD68_02160
BCL: ABC1280
BACP: SB24_04855
BACB: OY17_07705
BEO: BEH_12440
BGY: BGLY_1218
OIH: OB0380
BSE: Bsel_3091
ESI: Exig_1071
EAN: Eab7_1044
PMW: B2K_40340
JEO: JMA_14450
CCJ: UL81_01875(alcA)
GOR: KTR9_0498
SCO: SCO2016(SC7H2.30c) SCO2783(SCC105.14)
SCT: SCAT_1143 SCAT_p1224(alcA)
SALS: SLNWT_5882
STRP: F750_1862 F750_2609(desB)
SAMB: SAM23877_2102(rhbE) SAM23877_2818(desB)
SRW: TUE45_02530(iucD_1) TUE45_03398(iucD_2)
SLE: sle_44570(desB) sle_51230(sle_51230)
SRN: A4G23_01835(iucD_1) A4G23_04771(iucD_2)
SALF: SMD44_02199(iucD) SMD44_03081(iucD)
SALJ: SMD11_5041(iucD)
SLX: SLAV_23920(iucD1) SLAV_27510(iucD2) SLAV_39955(iucD3)
KSK: KSE_53820
CMS: CMS1134
CMC: CMN_02058(alcA)
ART: Arth_0286
ARX: ARZXY2_3649(iucD)
AAU: AAur_0267
ACH: Achl_0513
LMOI: VV02_14845
XCE: Xcel_1802
IDO: I598_1668(iucD)
CFL: Cfla_0197
CFI: Celf_3061
BLIN: BLSMQ_3011
KFL: Kfla_3184
PSIM: KR76_01465
NDA: Ndas_1073
FAL: FRAAL6424
AMD: AMED_2693(iucD)
AMN: RAM_13690
AMM: AMES_2665(iucD)
AMZ: B737_2666(iucD)
AMQ: AMETH_4715(iucD)
SESP: BN6_51340(iucD)
ACTI: UA75_12105
ACAD: UA74_12020
AHG: AHOG_11330(iucD)
ALL: CRK55946
AMS: AMIS_67510(sidC)
ASE: ACPL_7000(sidC)
ACTS: ACWT_6869
TBI: Tbis_3460
RXY: Rxyl_0408
AVA: Ava_2835
CPI: Cpin_2840
FLN: FLA_1337
FJO: Fjoh_3173
EAO: BD94_2299
ELB: VO54_00400(iucD)
CHZ: CHSO_0857
TJE: TJEJU_0710(alcA)
TMAR: MARIT_0170(tbsB)
HAL: VNG_6214G(hxyA)
HSL: OE_5098F(iucD)
HMU: Hmuk_3333
HME: HFX_5041(iucD)
HTU: Htur_0217
NAT: NJ7G_0328
 » show all
Taxonomy
Reference
1  [PMID:2518519]
  Authors
Plattner HJ, Pfefferle P, Romaguera A, Waschutza S, Diekmann H.
  Title
Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222.
  Journal
Biol Met 2:1-5 (1989)
Reference
2  [PMID:8504838]
  Authors
Macheroux P, Plattner HJ, Romaguera A, Diekmann H.
  Title
FAD and substrate analogs as probes for lysine N6-hydroxylase from Escherichia coli EN 222.
  Journal
Eur J Biochem 213:995-1002 (1993)
DOI:10.1111/j.1432-1033.1993.tb17846.x
Reference
3  [PMID:8218389]
  Authors
Thariath AM, Fatum KL, Valvano MA, Viswanatha T.
  Title
Physico-chemical characterization of a recombinant cytoplasmic form of lysine: N6-hydroxylase.
  Journal
Biochim Biophys Acta 1203:27-35 (1993)
DOI:10.1016/0167-4838(93)90032-M
Reference
4  [PMID:2935523]
  Authors
de Lorenzo V, Bindereif A, Paw BH, Neilands JB.
  Title
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
  Journal
J Bacteriol 165:570-8 (1986)
DOI:10.1128/JB.165.2.570-578.1986
  Sequence
Reference
5
  Authors
Marrone, L., Siemann, S., Beecroft, M. and Viswanatha, T.
  Title
Specificity of lysine:N-6-hydroxylase: A hypothesis for a reactive substrate intermediate in the catalytic mechanism.
  Journal
Bioorg Chem 24:401-406 (1996)
Reference
6  [PMID:2493814]
  Authors
Goh CJ, Szczepan EW, Menhart N, Viswanatha T.
  Title
Studies on lysine:N6-hydroxylation by cell-free systems of Aerobacter aerogenes 62-1.
  Journal
Biochim Biophys Acta 990:240-5 (1989)
DOI:10.1016/S0304-4165(89)80040-6
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.59
IUBMB Enzyme Nomenclature: 1.14.13.59
ExPASy - ENZYME nomenclature database: 1.14.13.59
BRENDA, the Enzyme Database: 1.14.13.59
CAS: 64295-82-5

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