KEGG   ENZYME: 1.14.13.59
Entry
EC 1.14.13.59               Enzyme                                 
Name
L-lysine N6-monooxygenase (NADPH);
lysine N6-hydroxylase;
L-lysine 6-monooxygenase (NADPH) (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
L-lysine,NADPH:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
L-lysine + NADPH + H+ + O2 = N6-hydroxy-L-lysine + NADP+ + H2O [RN:R00448]
Reaction(KEGG)
R00448
Substrate
L-lysine [CPD:C00047];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
N6-hydroxy-L-lysine [CPD:C01028];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). The enzyme from strain EN 222 of Escherichia coli is highly specific for L-lysine; L-ornithine and L-homolysine are, for example, not substrates.
History
EC 1.14.13.59 created 1999, modified 2001, modified 2012
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K03897  lysine N6-hydroxylase
Genes
EOIECO111_1296(iucD)
EOJECO26_1348(iucD)
ECOOECRM13514_1293(iucD)
ECOHECRM13516_1249(iucD)
ESLO3K_23070
ESOO3O_02285
ESMO3M_22985
ECKEC55989_4642(iucD)
ENAECNA114_4698(iucD)
ECOSEC958_3274(iucD)
ECVAPECO1_O1CoBM169(iucD)
ECMEcSMS35_3179(iucD)
ECQECED1_4902(iucD)
EUMECUMN_3372(iucD)
ECTECIAI39_3426(iucD)
EOCCE10_3449(iucD)
ECZECS88_p0010(iucD)
ECCc3624(iucD)
ELNNRG857_30237(iucD)
EABECABU_c32820(iucD)
ELCi14_3317(iucD)
ELDi02_3317(iucD)
ECOJP423_16375
ERUYOSH18_13360(iucD)
SEMSTMDT12_C38540
SESSARI_02155
SFLSF3718(iucD)
SFXS4053(iucD)
SFVSFV_3851(iucD)
SFESFxv_4051
SFNSFy_5297
SFSSFyv_5371
SFTNCTC1_04015(iucD)
SSNSSON_3604(iucD)
SBOSBO_4340(iucD)
SBCSbBS512_E4631(iucD)
ENCECL_03319(iucD)
ENLA3UG_14870
ECLGEC036_28790
ECLEECNIH2_14835
ECLNECNIH4_08320
ECLIECNIH5_13755
ECLXLI66_14275
ECLZLI64_13685
EHMAB284_11220
EXFBFV63_14020
ECLAECNIH3_13810
ECLCECR091_13745
EAUDI57_04915
EKBBFV64_14425
ENOECENHK_14315
EECEcWSU1_02922(iucD)
ELGBH714_10665
ERNBFV67_13935
ECLSLI67_011575
ECHGFY206_15845
ESHC1N69_14700
EPTHWQ17_21545
ENXNI40_014285
LNICWR52_20770
EBGFAI37_14855
ENDA4308_19000
ENZG0034_14905
ENSHWQ15_22485
ENKLOC22_02265
EMORL6Y89_13945
ENBELK40_14625
EQUOM418_14010
EPUQVH39_14955
ESAESA_pESA3p05550
CSZCSSP291_21033
CSJCSK29544_1p0026
CCONAFK62_20065
CDMAFK67_21005
CSIP262_p1011
CMJAFK66_020210
CUIAFK65_20165
CTUCtu_1p01070(iucD)
KPUKP1_p315(iucD)
KPOKPN2242_25266
KPRKPR_4894(iucD)
KPNKBN49_pII0065(iucD)
KMIVW41_08305
CKOCKO_00970
CNTJT31_04210
CEMLH23_13810
CENLH86_13120
CLAPNCTC11466_02784(iucD)
YREHEC60_04360
TOEQMG90_12575
YPEYPO0993(iucD)
YPKy3384(iucD)
YPHYPC_0799(iucD)
YPAYPA_0271
YPNYPN_3191
YPMYP_3447(iucD)
YPPYPDSF_0525
YPZYPZ3_0745(iucD)
YPTA1122_00010
YPDYPD4_0700(iucD)
YPXYPD8_0700(iucD)
YPWCH59_870(iucD)
YPJCH55_3547(iucD)
YPVBZ15_2580(iucD)
YPLCH46_4152(iucD)
YPSYPTB3266(iucD)
YPOBZ17_3344(iucD)
YPIYpsIP31758_0711(iucD)
YPYYPK_0783
YPBYPTS_3402
YPQDJ40_3171(iucD)
YPUBZ21_2546(iucD)
YPRBZ20_2848(iucD)
YPCBZ23_2827(iucD)
YPFBZ19_2672
YSIBF17_03350
YINCH53_2434(iucD)
YAKACZ76_16795
YMADA391_18850
YMOHRD69_17565
SMARSM39_3651 SM39_4454(iucD)
SMACSMDB11_3458 SMDB11_4148(iucD)
SMWSMWW4_v1c41520
SPESpro_0025 Spro_0919 Spro_2520
SRRSerAS9_0844 SerAS9_2495
SRLSOD_c07750(iucD1) SOD_c23600(iucD2) SOD_c47380(iucD)
SRYM621_04275 M621_12890 M621_25650
SPLYQ5A_004125(iucD_1) Q5A_013040(iucD_2)
SRSSerAS12_0844 SerAS12_2496
SRASerAS13_0844 SerAS13_2497
SMAFD781_0884
SLQM495_03880 M495_12465 M495_24705
SERFL085_07285
SERSSERRSCBI_00130 SERRSCBI_20200
SFWWN53_13455
SFGAV650_09005
SRZAXX16_4217
SERMCLM71_04285
SQUE4343_03620 E4343_11905 E4343_16150
SFJSAMEA4384070_0951(iucD)
SOFNCTC11214_00581(iucD)
SSURATE40_017480
SURIJ0X03_03140
SRHZFO014_05495
SENPKHA73_04105 KHA73_23070
SNEMNLX84_21450
SNEVOI978_10090
SGRISGBXF1_00041(iucD_1) SGBXF1_00843(iucD_2) SGBXF1_02537(iucD_3)
RAHRahaq_3478
RACEJHW33_11610
RAQRahaq2_3554
RAAQ7S_17510
ROXBV494_12445
RVCJ9880_03080
RBONQNM34_17835
RIUI2123_19285
FSMCCS41_02975
EAMEGXP68_03935
RBADH2866_12030
RCBO1V66_13555
PWAPecwa_0950
PPARA8F97_14180
PECW5S_0841
PCACOI450_13395(iucD)
DDADd703_3091
BRBEH207_14280
LBQCKQ53_00780
LPOPI6N93_03520
ETORIN69_18765
EGEEM595_p0529(iucD)
PAMPANA_0353(iucD)
PLFPANA5342_4061(iucD)
PAJPAJ_3508(iucD)
PAQPAGR_g3924
PSTWDSJ_03460
KPIEN5580_21625
VCYIX92_17060
VCTJV59_21965
VSIMTO69_17220
VMIAL543_14495
VSHBSZ05_19030
VKABTD91_15885
VOSKNV97_12545
VTICEQ48_09235
VMTQYQ96_04685
VFIVF_A0164(iucD)
VFMVFMJ11_A0200
VSAVSAL_II0278(iucD)
GHOAL542_16535
SALYE8E00_15545
SCOTHBA18_15435
SPRON7E60_14040
PSTPSPTO_0211(iucD)
PAVLBKM03_01095
PVRPverR02_14275
PSKU771_15470
PXNHU772_013785
PMUYKSS95_16080
PAGRE2H98_15805
KAKKalk_08725
SWDSwoo_0586
PAEWKIH87_11625
AGQLQZ07_20620
AGAROAG1_07840(iucD)
MMAAFR932_14170
SPOIIMCC21906_00377
MICTFIU95_16360(iucD)
MICZGL2_22490(iucD)
MVBMJO52_16665
ASIPAQUSIP_25460(iucD)
LPOLPO_2065(iucD)
LPFlpl1946
LHALHA_1351(iucD)
LSHCAB17_06275
LCJNCTC11976_01019(iucD)
MISZMishRS11D_43100(iucD)
NOCNoc_1811
NWANwat_1301
HTXEKK97_08780
KUSB9G99_09250
KMAB9H00_05600
KUYFY550_02650
PAURFGL86_03615
SAJSQO259_04305
EEINX720_08185
ENGO2T12_00705
KKOKkor_2040
KGETQ33_0707
CHROCXB49_00465
CRZD1345_06150
CHAECH06BL_13350
MAERDAI18_18595
BCJBCAS0043
BCEOI35_7041
BCEDDM42_6412
BSTLBBJ41_34310
BARINLX30_34745
BURKDM992_33275
BPYBphyt_0280
BCAIK788_0006333
PSPWBJG93_16680
PBRYNDK50_34985
OURCEQ07_01965
CPRACPter91_0284
MANCIV454_24120
MLOmlr5923
MLNA9174_32405
MCIMesci_5807
MCICA4R28_21890
MOPMesop_6383
MAMMesau_05856
MAMOA6B35_31120
MESWA9K65_031345
MESMEJ066_12270
MESPC1M53_02775
MJREB229_33125
MERDEB233_30775
MOHIHQ72_34065
MMEILRP31_09865
NTUNTH_01028(iucD)
AMISAmn_11140
PHTBLM14_29930
PHYLHB779_01090
KMNHW532_05380
AVVRvVAT039_40620
AVFRvVAR031_33170
AVIAvi_5731
RETRHE_PF00456(vbsO)
RECRHECIAT_PC0000681(vbsO)
RELREMIM1_PF00420(iucD)
REPIE4803_PC00508(iucD)
REIIE4771_PD00518(iucD)
RLEpRL120314(vbsO)
RLTRleg2_4809
RLBRLEG3_07065
RLURLEG12_10340
RTRRTCIAT899_PC05660
RHNAMJ98_PC00365 AMJ98_PE00534(iucD)
RPHAAMC79_PC00336 AMC79_PD00672(iucD)
RHXAMK02_PC00354 AMK02_PD00403(iucD)
RHKKim5_PA00462 Kim5_PC00465(iucD)
REZAMJ99_PB00359 AMJ99_PD00534(iucD)
RHRCKA34_19735
RADCO657_27950
RIIFFM53_025065
RHIDFFM81_025100
RBQJ2J99_23410
RLNJ0663_24075
RBANJ2J98_22760
RAWNE851_32990
RLWRlegWSM1455_25315
ARAArad_8229(vbsO)
RBWRLCC275e_24815
RHIZQMO80_004871
OANOant_3254
OAHDR92_3235(iucD)
OCRHGK82_18830
OCLGTN27_19035
BJAblr2131(blr2131)
BJUBJ6T_77190
BJPRN69_37485
BRCBCCGELA001_30200
BGZXH91_07535
BELBE61_77460(iucD)
BSEPHAP48_0005940
BBANJ4G43_043230
BAUTQA635_32100
BBRAQA636_33715
BXNI3J27_33210
BVVBHK69_19665
AZCAZC_3247
LNEFZC33_08125
METM446_5059
METSDK389_06835
MSLMsil_3318
HDIHDIA_3128(iucD)
PPHRAPZ00_04765
CONTQ29_06785
THAACFI11_19950
PFISJHX87_10370
POZI0K15_07675
GBEGbCGDNIH1_2375
GBHGbCGDNIH2_2375
GBCGbCGDNIH3_2375
GBSGbCGDNIH4_2375
TXITH3_13065
THAIIT893_15040
CCROCMC5_023430
NANNO0S08_38350
BBABd1573
BBATBdt_1566
BBACEP01_04085
BDZDOM22_13135
BDKHW988_12135
BMXBMS_0525(iucD)
HAXBALOs_0964(iucD)
BSTOC0V70_12470
PSTISOO65_15530
NSLBOX37_20165
NTPCRH09_08345
NOZDMB37_12225
NAHF5544_07265
RHARHA1_ro08654
ROAPd630_LPD03701
SANLKZO11_35730
SGRFSGFS_066720
SCIB446_31510
STREGZL_09053
SLDT261_7621
SLCSL103_05005
STSIA4E84_35470
SNRSNOUR_40995
SPUNBFF78_29745
SGVB1H19_09125
SLXSLAV_39955(iucD3)
STROSTRMOE7_14925
SGEDWG14_06029(iucD_2)
SQZFQU76_11675
SVRCP971_29835
STSUB7R87_11320 B7R87_30270
SMOBJ7W19_03315
SGJIAG43_25475
SINEKI385_07805
SYUNMOV08_42040
SJNRI060_04845
SHAUK9S39_10135
RIAC7V51_07215
KFLKfla_2932
KQIF1D05_35110
FRAFrancci3_3275
FSYFsymDg_2883
ACYNORV05_13005
SESPBN6_51340(iucD)
KBUQ4V64_41730
SAQSare_0571 Sare_1046
MAUMicau_3806
MILML5_4610
MICBMicB006_2881
MTUACSH63_06350
MCHLPVK74_09360
MPRNQ3V37_03980
PLKCIK06_17640
DVCDvina_35560 Dvina_40770
DMATDmats_30405 Dmats_35660
CAICaci_6320
NAVJQS30_08325
FNEFSDG_01143
FOEJJC03_16525
KANIMCC3317_12960(iucD)
 » show all
Reference
1  [PMID:2518519]
  Authors
Plattner HJ, Pfefferle P, Romaguera A, Waschutza S, Diekmann H.
  Title
Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222.
  Journal
Biol Met 2:1-5 (1989)
DOI:10.1007/bf01116193
Reference
2  [PMID:8504838]
  Authors
Macheroux P, Plattner HJ, Romaguera A, Diekmann H.
  Title
FAD and substrate analogs as probes for lysine N6-hydroxylase from Escherichia coli EN 222.
  Journal
Eur J Biochem 213:995-1002 (1993)
DOI:10.1111/j.1432-1033.1993.tb17846.x
Reference
3  [PMID:8218389]
  Authors
Thariath AM, Fatum KL, Valvano MA, Viswanatha T.
  Title
Physico-chemical characterization of a recombinant cytoplasmic form of lysine: N6-hydroxylase.
  Journal
Biochim Biophys Acta 1203:27-35 (1993)
DOI:10.1016/0167-4838(93)90032-M
Reference
4  [PMID:2935523]
  Authors
de Lorenzo V, Bindereif A, Paw BH, Neilands JB
  Title
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
  Journal
J Bacteriol 165:570-8 (1986)
DOI:10.1128/JB.165.2.570-578.1986
  Sequence
Reference
5
  Authors
Marrone, L., Siemann, S., Beecroft, M. and Viswanatha, T.
  Title
Specificity of lysine:N-6-hydroxylase: A hypothesis for a reactive substrate intermediate in the catalytic mechanism.
  Journal
Bioorg Chem 24:401-406 (1996)
Reference
6  [PMID:2493814]
  Authors
Goh CJ, Szczepan EW, Menhart N, Viswanatha T.
  Title
Studies on lysine:N6-hydroxylation by cell-free systems of Aerobacter aerogenes 62-1.
  Journal
Biochim Biophys Acta 990:240-5 (1989)
DOI:10.1016/S0304-4165(89)80040-6
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.59
IUBMB Enzyme Nomenclature: 1.14.13.59
ExPASy - ENZYME nomenclature database: 1.14.13.59
BRENDA, the Enzyme Database: 1.14.13.59
CAS: 64295-82-5

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