KEGG   ENZYME: 1.14.13.81Help
Entry
EC 1.14.13.81               Enzyme                                 

Name
magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase;
Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
magnesium-protoporphyrin-IX 13-monomethyl ester,NADPH:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 = 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O (overall reaction) [RN:R10068];
(1a) magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O [RN:R06265];
(1b) 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O [RN:R06266];
(1c) 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 3,8-divinyl protochlorophyllide a + NADP+ + 2 H2O [RN:R06267]
Reaction(KEGG)
Substrate
magnesium-protoporphyrin IX 13-monomethyl ester [CPD:C04536];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
Product
3,8-divinyl protochlorophyllide a [CPD:C11831];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
Comment
Requires Fe(II) for activity. The enzyme participates in the biosynthesis of chlorophyllide a in aerobic organisms. The same transformation is achieved in anaerobic organisms by EC 1.21.98.3, anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase. Some facultative phototrophic bacteria, such as Rubrivivax gelatinosus, possess both enzymes.
History
EC 1.14.13.81 created 2003, modified 2017
Pathway
ec00860  Porphyrin and chlorophyll metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K04035  magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase
Genes
ATH: AT3G56940(CRD1)
ALY: ARALYDRAFT_486137
CRB: 17887096
CSAT: 104712185 104712356 104732803 104781590 104788276 104791984
EUS: EUTSA_v10006009mg
BRP: 103830076 103841596 103863337
BNA: 106368040 106405693 106445907 111205305 111208747
BOE: 106299791 106312794 106339440
THJ: 104803235 104824384
CPAP: 110811309
CIT: 102612985
TCC: 18598690
GRA: 105786192
EGR: 104443639
VRA: 106753941
VAR: 108329545
CCAJ: 109802482
CAM: 101494559
ADU: 107494229
AIP: 107604718
LJA: Lj1g3v4058630.1(Lj1g3v4058630.1)
FVE: 101306835
PPER: 18770050
PMUM: 103339106
PAVI: 110763662
ZJU: 107423584
CSV: 101219179
CMO: 103502893
MCHA: 111021778
RCU: 8289525
JCU: 105648924
HBR: 110655527
VVI: 100241461
SLY: 101257518(at103)
SPEN: 107002129
SOT: 102600334(at103)
CANN: 107844048
NSY: 104227011
NTO: 104089135
INI: 109161721(PNIL34)
HAN: 110884853
LSV: 111879482
BVG: 104889639
NNU: 104593560
OSA: 4326901
DOSA: Os01t0279100-01(Os01g0279100)
OBR: 102712668
ATS: 109776579(LOC109776579)
SBI: 8074544
SITA: 101773118
PDA: 103698135
EGU: 105036097
DCT: 110113521
AOF: 109848727
ATR: 18426189
MNG: MNEG_2725
APRO: F751_5195
HYR: BSY239_1293(acsF)
RGE: RGE_33550(acsF)
METR: BSY238_2458(acsF)
AGC: BSY240_3656(acsF)
BRA: BRADO1649(acsF)
BBT: BBta_6407(acsF)
BRS: S23_19480(acsF)
AOL: S58_58890
BRO: BRAD285_5752(acsF)
RPA: RPA1552
RPB: RPB_3971
RPC: RPC_1323
RPD: RPD_3726
RPE: RPE_1357
RPT: Rpal_1740
BOS: BSY19_4045(acsF)
MEX: Mext_4806
MEA: Mex_1p5263(acsF)
MDI: METDI5863(acsF)
MCH: Mchl_5273
MET: M446_3728
MPO: Mpop_5351
MOR: MOC_2242(acsF)
META: Y590_24560
MEE: DA075_04100(acsF)
MSL: Msil_2032
RSP: RSP_0294
JAN: Jann_0151(acsF)
RDE: RD1_0129(acsF)
RLI: RLO149_p940810(acsF)
DSH: Dshi_3544(acsF)
PTP: RCA23_c29820(acsF)
RSU: NHU_00221(acsF)
RHC: RGUI_1977
RBG: BG454_02870(acsF)
GEH: HYN69_05365(acsF)
SPHI: TS85_22955
SSAN: NX02_18395
BLAS: BSY18_2698(acsF)
PHZ: CHX26_14625(acsF)
AMV: ACMV_19060(acsF)
RCE: RC1_2105(acsF)
NCB: C0V82_18105(acsF)
SYN: sll1214 sll1874(AT103)
SYZ: MYO_116600(at103) MYO_150
SYY: SYNGTS_0005(sll1214) SYNGTS_1645(chlAII)
SYT: SYNGTI_0005(sll1214) SYNGTI_1645(chlAII)
SYS: SYNPCCN_0005(sll1214) SYNPCCN_1644(chlAII)
SYQ: SYNPCCP_0005(sll1214) SYNPCCP_1644(chlAII)
SYJ: D082_08250(AT103) D082_11950(ycf59)
SYO: C7I86_00025(acsF) C7I86_08535(acsF)
SYW: SYNW1198(PNIL34)
SYC: syc2188_d(ycf59)
SYG: sync_1304(acsF)
SYR: SynRCC307_1476(acsF)
SYX: SynWH7803_1102(acsF)
CYA: CYA_2703(acsF)
CYB: CYB_1254(acsF)
SYNR: KR49_02360
SYND: KR52_09945
SYNW: SynWH8103_01332(acsF)
TEL: tlr1426(ycf59) tlr1722
THN: NK55_02575(acsF1) NK55_05260(acsF2)
CYI: CBM981_0231(acsF) CBM981_1951(acsF)
HHG: XM38_022230(acsF3) XM38_048020(acsF1)
PMA: Pro_0992(acsF)
PMM: PMM0844(PNIL34)
PMT: PMT_2196
PMB: A9601_10251(pniL34)
PMC: P9515_09201(pnilL34)
PMF: P9303_29201(pniL34)
PMG: P9301_10241(pniL34)
PMH: P9215_10571(pniL34)
PMJ: P9211_09191(pniIL34)
PME: NATL1_08941(pnil34)
PRC: EW14_1059
PRM: EW15_0910
AMR: AM1_0465(acsF) AM1_2295(acsF)
CYL: AA637_00120(acsF) AA637_14735(acsF-2)
TER: Tery_0728
ARP: NIES39_B00770(acsF)
GVI: gvip493(ycf59)
GLJ: GKIL_2292(acsF)
NOE: CLI64_10670(acsF) CLI64_21635(acsF) CLI64_28005(acsF)
ANB: ANA_C10475(acsF) ANA_C11197(acsF)
NSP: BMF81_00211(acsF) BMF81_04265(acsF_2)
CTHE: Chro_1308
RCA: Rcas_1541
CAU: Caur_2590
CAG: Cagg_1285
 » show all
Taxonomy
Reference
1  [PMID:3202840]
  Authors
Walker CJ, Mansfield KE, Rezzano IN, Hanamoto CM, Smith KM, Castelfranco PA
  Title
The magnesium-protoporphyrin IX (oxidative) cyclase system. Studies on the mechanism and specificity of the reaction sequence.
  Journal
Biochem J 255:685-92 (1988)
Reference
2  [PMID:12226378]
  Authors
Bollivar DW, Beale SI.
  Title
The Chlorophyll Biosynthetic Enzyme Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase (Characterization and Partial Purification from Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803).
  Journal
Plant Physiol 112:105-114 (1996)
Reference
3  [PMID:11790744]
  Authors
Pinta V, Picaud M, Reiss-Husson F, Astier C
  Title
Rubrivivax gelatinosus acsF (previously orf358) codes for a conserved, putative binuclear-iron-cluster-containing protein involved in aerobic oxidative cyclization of Mg-protoporphyrin IX monomethylester.
  Journal
J Bacteriol 184:746-53 (2002)
DOI:10.1128/JB.184.3.746-753.2002
Reference
4  [PMID:14673103]
  Authors
Tottey S, Block MA, Allen M, Westergren T, Albrieux C, Scheller HV, Merchant S, Jensen PE
  Title
Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required  for the synthesis of protochlorophyllide.
  Journal
Proc Natl Acad Sci U S A 100:16119-24 (2003)
DOI:10.1073/pnas.2136793100
  Sequence
[ath:AT3G56940]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.81
IUBMB Enzyme Nomenclature: 1.14.13.81
ExPASy - ENZYME nomenclature database: 1.14.13.81
BRENDA, the Enzyme Database: 1.14.13.81
CAS: 92353-62-3

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