KEGG   ENZYME: 1.14.13.82Help
Entry
EC 1.14.13.82               Enzyme                                 

Name
vanillate monooxygenase;
4-hydroxy-3-methoxybenzoate demethylase;
vanillate demethylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
vanillate:oxygen oxidoreductase (demethylating)
Reaction(IUBMB)
vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde [RN:R05274]
Reaction(KEGG)
Substrate
vanillate [CPD:C06672];
O2 [CPD:C00007];
NADH [CPD:C00004];
H+ [CPD:C00080]
Product
3,4-dihydroxybenzoate [CPD:C00230];
NAD+ [CPD:C00003];
H2O [CPD:C00001];
formaldehyde [CPD:C00067]
Comment
Forms part of the vanillin degradation pathway in Arthrobacter sp.
History
EC 1.14.13.82 created 2000 as EC 1.2.3.12, transferred 2003 to EC 1.14.13.82
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K03862  vanillate monooxygenase
Genes
PAE: PA4904(vanA)
PAEV: N297_5074
PAEI: N296_5074
PAU: PA14_64800(vanA)
PAP: PSPA7_5628(vanA)
PAG: PLES_52901(vanA)
PAF: PAM18_5015(vanA)
PNC: NCGM2_0620(vanA)
PAEB: NCGM1900_5646(vanA)
PDK: PADK2_26010
PAEP: PA1S_26620
PAEM: U769_26885
PAEL: T223_27080
PAEU: BN889_03196(vanA)
PAEG: AI22_07375
PAEC: M802_5072
PAEO: M801_4939
PRE: PCA10_51390(vanA)
PCQ: PcP3B5_56530(tsaM1_3)
PPU: PP_3736(vanA)
PPF: Pput_2027
PPI: YSA_09409
PPX: T1E_1717(vanA)
PPUT: L483_19445
PPUN: PP4_20850(vanA)
PST: PSPTO_2904(vanA)
PSB: Psyr_2708
PSYR: N018_13725
PSP: PSPPH_2475(vanA)
PFL: PFL_3471(vanA)
PPRC: PFLCHA0_c35050(vanA)
PPRO: PPC_3614
PFS: PFLU_3293
PSTT: CH92_04375
PSES: PSCI_1617
PSEM: TO66_13045
PSOS: POS17_3599
ACB: A1S_1116
ABY: ABAYE2623(vanA)
ABN: AB57_1200
ABAZ: P795_11745
ABAU: IX87_05265
ABAA: IX88_07830
ACC: BDGL_000396(vanA)
ACI: ACIAD0980(vanA)
PAT: Patl_0844
CSA: Csal_0344
HEL: HELO_3940(vanA)
HAM: HALO0093
RSO: RSp0222(vanA)
RSL: RPSI07_mp0173(vanA)
RSN: RSPO_m00210(vanA)
RSM: CMR15_mp10192(vanA)
RSE: F504_3661
BVE: AK36_3260
BCN: Bcen_3124
BCJ: BCAM2436(vanA)
BCEN: DM39_5402
BCEW: DM40_3099
BCEO: I35_6336(vanA)
BAM: Bamb_4591
BMU: Bmul_3214
BMK: DM80_4594
BMUL: NP80_3339
BCT: GEM_3253
BCED: DM42_5545
BDL: AK34_5017
BCON: NL30_16005
BLAT: WK25_28075
BTEI: WS51_09170
BSEM: WJ12_32220
BPSL: WS57_01935
BMEC: WJ16_30710
BSTG: WT74_12840
BGU: KS03_4484
BUK: MYA_3124
PPNO: DA70_15150
PPNM: LV28_15530
PPUL: RO07_09575
PSPU: NA29_05490
PAPI: SG18_10075
RFR: Rfer_0280
PNA: Pnap_2725
ACRA: BSY15_1230
VEI: Veis_1485
DAC: Daci_1352
VPD: VAPA_2c01950(vanA)
CTES: O987_24985
LIM: L103DPR2_01485(tsaM1)
LIH: L63ED372_00786(tsaM1_1)
CBAA: SRAA_2261
CBAB: SMCB_0812
HSE: Hsero_3992(vanA)
HRB: Hrubri_3944(vanA)
LCH: Lcho_1335
TCL: Tchl_3188
CCR: CC_2393
CCS: CCNA_02476(vanA)
CSE: Cseg_2340
BLAS: BSY18_3501
 » show all
Taxonomy
Reference
1  [PMID:3170489]
  Authors
Brunel F, Davison J.
  Title
Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase.
  Journal
J Bacteriol 170:4924-30 (1988)
DOI:10.1128/JB.170.10.4924-4930.1988
  Sequence
Reference
2  [PMID:9098058]
  Authors
Priefert H, Rabenhorst J, Steinbuchel A.
  Title
Molecular characterization of genes of Pseudomonas sp. strain HR199 involved in bioconversion of vanillin to protocatechuate.
  Journal
J Bacteriol 179:2595-607 (1997)
DOI:10.1128/JB.179.8.2595-2607.1997
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.82
IUBMB Enzyme Nomenclature: 1.14.13.82
ExPASy - ENZYME nomenclature database: 1.14.13.82
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.82
BRENDA, the Enzyme Database: 1.14.13.82
CAS: 39307-11-4

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