KEGG ENZYME: 1.14.13.82

ENZYME: 1.14.13.82

Entry

EC 1.14.13.82               Enzyme

Name

vanillate monooxygenase;
4-hydroxy-3-methoxybenzoate demethylase;
vanillate demethylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

vanillate:oxygen oxidoreductase (demethylating)

Reaction(IUBMB)

vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde [RN:R05274]

Reaction(KEGG)

R05274

Substrate

vanillate [CPD:C06672];
O2 [CPD:C00007];
NADH [CPD:C00004];
H+ [CPD:C00080]

Product

3,4-dihydroxybenzoate [CPD:C00230];
NAD+ [CPD:C00003];
H2O [CPD:C00001];
formaldehyde [CPD:C00067]

Comment

Forms part of the vanillin degradation pathway in Arthrobacter sp.

History

EC 1.14.13.82 created 2000 as EC 1.2.3.12, transferred 2003 to EC 1.14.13.82

Pathway

ec00627

Aminobenzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K03862

vanillate monooxygenase

Genes

XCC:	XCC0363(vanA)

XCB:

XC_0375

XCA:	xcc-b100_0389

XCP:

XCR_4152

XCV:

XCV0377

XAX:

XACM_0351

XAC:	XAC0363(vanA)

XCI:	XCAW_00770(vanA)

XCT:	J151_00485

XCJ:	J158_00482

XCU:	J159_00482

XCN:	J169_00481

XCW:	J162_00481

XCR:	J163_00481

XCM:	J164_00481

XCF:	J172_00476

XFU:	XFF4834R_chr03470

XAO:	XAC29_01875

XOR:

XOC_4549

XOZ:	BE73_00785

XAL:	XALC_3034(vanA)

XSA:	SB85_14095

XVE:	BJD12_14550

XPE:	BJD13_15130

XHR:	XJ27_17630

XGA:	BI317_22060

XPH:	XppCFBP6546_10005(XppCFBP6546P_10005)

XAN:	AC801_01705

XAR:	XB05_18445

XCZ:	EBN15_01815

XTH:	G4Q83_18280

XEU:	XSP_000373

XHD:	LMG31886_04100(tsaM1)

XPR:	MUG10_08460

XDY:	NYR95_02090

XHA:	PG878_01515

XRY:	QN244_01455

VOS:	KNV97_08815

PAE:	PA4904(vanA)

PAEV:

N297_5074

PAEI:

N296_5074

PAU:	PA14_64800(vanA)

PAP:	PSPA7_5628(vanA)

PAG:	PLES_52901(vanA)

PAF:	PAM18_5015(vanA)

PNC:	NCGM2_0620(vanA)

PAEB:

NCGM1900_5646(vanA)

PDK:	PADK2_26010

PSG:	G655_25860

PRP:	M062_25870

PAEP:

PA1S_26620

PAER:

PA1R_gp2826

PAEM:

U769_26885

PAEL:

T223_27080

PAES:

SCV20265_5564

PAEU:

BN889_03196(vanA)

PAEG:

AI22_07375

PAEC:

M802_5072

PAEO:

M801_4939

SECH:

B18_11340

PRE:	PCA10_51390(vanA)

PCQ:	PcP3B5_56530(tsaM1_3)

PPAA:

B7D75_25510

PPU:	PP_3736(vanA)

PPF:

Pput_2027

PPG:	PputGB1_2170

PPW:	PputW619_2045

PPB:	PPUBIRD1_2026

PPI:

YSA_09409

PPX:	T1E_1717(vanA)

PPUT:

L483_19445

PPUN:

PP4_20850(vanA)

PFV:	Psefu_4050

POR:	APT59_07750

PPSL:

BJP27_22580

PALD:

LU682_011510

PST:	PSPTO_2904(vanA)

PSB:

Psyr_2708

PSYR:

N018_13725

PSP:	PSPPH_2475(vanA)

PSAV:

PSA3335_15475

PAMG:

BKM19_016235

PAVL:

BKM03_14475

PVD:	CFBP1590__2812(vanA)

PCAB:

JGS08_12860

PCOF:

POR16_12565

PTRE:

I9H09_12500

PSYI:

MME58_14090

PAST:

N015_13745

PFL:	PFL_3471(vanA)

PPRC:

PFLCHA0_c35050(vanA)

PPRO:

PPC_3614

PFE:	PSF113_2802

PZA:	HU749_016095

PFS:

PFLU_3293

PPZ:	H045_05725

PTV:	AA957_00695

PCG:	AXG94_01515

PVR:	PverR02_12230

PMED:

E3Z27_15085

PMUD:

NCTC8068_02156(kshA_1)

PMAB:

U0037_16460

PLUL:

FOB45_22220

PBA:	PSEBR_a2987

PBC:	CD58_14310

PDR:	H681_21940

PSV:	PVLB_10605

PSK:	U771_11805

PCH:	EY04_12300

PCZ:	PCL1606_35110

PCP:	JM49_17570

PCHP:

C4K32_2627

PFZ:	AV641_10035

PSES:

PSCI_1617

PSEM:

TO66_13045

PPSY:

AOC04_06905

PSOS:

POS17_3599

PUM:	HGP31_09950

POJ:	PtoMrB4_29730(vanA)

PPSH:

G5J76_09050 G5J76_17005

PVK:	EPZ47_14290

PEZ:	HWQ56_13535

PBZ:	GN234_02485

PMAO:

PMYSY11_2124(vanA)

PASG:

KSS96_11865

PALV:

KSS97_15845

PCAS:

LOY40_15195

PJU:	L1P09_14540

PKM:	PZ739_11030

PTAI:

ICN73_22530

PKK:	QQ992_10500

PCUC:

PSH97_15370

PTRL:

OU419_25130

PPAE:

LDL65_24295

PRHZ:

CRX69_17870

PSJY:

AA098_17195

PSKU:

KUIN1_26820

PHEF:

PSH57_15000

PSLP:

BHQ29_09540

PFOU:

OZ911_17110

PBEJ:

PSH84_18555

PSTT:

CH92_04375

ACB:

A1S_1116

ABY:	ABAYE2623(vanA)

ABN:

AB57_1200

ABB:	ABBFA_02396(tsaM1_1)

ABAB:

BJAB0715_01306

ABAZ:

P795_11745

ABAU:

IX87_05265

ABAA:

IX88_07830

ABAL:

ABLAC_19570

ACC:	BDGL_000396(vanA)

ANO:	RR32_12590

ALC:	OTEC02_13335

ACAL:

BUM88_05435

ASEI:

I8T81_13365

ACD:	AOLE_13720

ACI:	ACIAD0980(vanA)

AEI:	AOY20_07280

ACW:	A0J50_11960

AHL:	AHTJS_03680

ASOL:

BEN76_08720

AGU:	AS4_34520(vanA)

AUG:

URS_1853

ATN:	FM020_02805

ATZ:	M5E07_08245

AVIV:

LF296_12220

ACIE:

KIP84_12870

ASUE:

F2A31_03660

ACIG:

BS636_01275

AAW:	AVL56_08685

ALR:	DS731_00575

PAT:

Patl_0844

ALII:

QR722_00590

CSA:

Csal_0344

HEL:	HELO_3940(vanA)

HAM:

HALO0093

HMD:	CTT34_17620

HSV:	HNO53_19215

HPRO:

LMS44_23210

HBH:	E4T21_20970

HASO:

B2G49_18375

HCAM:

I4484_18900

HNP:	SR894_21770

HTT:	HZS52_05285

HSX:	HNO51_18955

HTX:	EKK97_21215

SAJS:

QO259_02910

MMW:	Mmwyl1_1296

MME:	Marme_1172

MPC:	Mar181_2609

MPRI:

MP3633_2749

MARD:

IBG28_05285

MFOI:

JSY38_04510

MPRF:

J8N69_01790

MRHI:

KDW99_05575

MPON:

MACH16_08910(vanA)

MARL:

HH196_04930

MRZ:	KDW95_00635

GSN:	YC6258_03914

SALN:

SALB1_1089

NBL:	GJV52_04080

VIT:	ADP71_28920

VST:	LVJ81_00220

RSO:	RSp0222(vanA)

RSE:

F504_3661

RPU:	CDC45_18725

RNC:	GO999_23120

RSL:	RPSI07_mp0173(vanA)

RSN:	RSPO_m00210(vanA)

RSM:	CMR15_mp10192(vanA)

RSY:	RSUY_35550(tsaM1)

CBW:	RR42_s0708 RR42_s2469

CCUP:

BKK81_20160

CUP:	BKK80_21645

CUU:	BKK79_26810

BVI:	Bcep1808_3498

BVE:

AK36_3260

BUR:	Bcep18194_B0420

BCM:	Bcenmc03_5029

BCH:	Bcen2424_5243

BCJ:	BCAM2436(vanA)

BCEN:

DM39_5402

BCEW:

DM40_3099

BCEO:

I35_6336(vanA)

BAM:

Bamb_4591

BAC:	BamMC406_5124

BMJ:	BMULJ_05311(vanA)

BMU:

Bmul_3214

BMK:

DM80_4594

BMUL:

NP80_3339

BCT:

GEM_3253

BCED:

DM42_5545

BCEP:

APZ15_22685

BDL:

AK34_5017

BPYR:

ABD05_21690

BCON:

NL30_16005

BDF:	WI26_25000

BLAT:

WK25_28075

BTEI:

WS51_09170

BSEM:

WJ12_32220

BPSL:

WS57_01935

BMEC:

WJ16_30710

BSTL:

BBJ41_26115

BANN:

JFN94_16415

BARI:

NLX30_24955

BAEN:

L3V59_27000

BGL:	bglu_2g14550

BGU:

KS03_4484

BUK:

MYA_3124

BURK:

DM992_23760

BGE:	BC1002_3291

PSPW:

BJG93_19170

PCAF:

DSC91_002445

PACP:

FAZ97_28965

PHUN:

U0042_05490

PPK:	U875_22470

PPNO:

DA70_15150

PPNM:

LV28_15530

PRB:	X636_11695

PPUL:

RO07_09575

PSPU:

NA29_05490

PAPI:

SG18_10075

PVE:	UC34_01360

POX:	MB84_05695

PFG:	AB870_12615

PNR:	AT302_09260

PAND:

DRB87_01225

PFIB:

PI93_001180

PCOM:

NTU39_01585

TVL:	FAZ95_34465

TCAR:

U0034_04805

BOJ:	CBF45_16195

PUD:	G9Q38_04025 G9Q38_10650 G9Q38_11445 G9Q38_13355

CDN:	BN940_08501

PHN:	PAEH1_08445

PIG:	EGT29_00385

KGY:	EHF36_16230

NARM:

N7E01_02480

RFR:

Rfer_0280

RSB:	RS694_12765

RHY:	RD110_11775

RHF:	EUB48_13000

RHOA:

HZ993_17560

RHOB:

HTY51_17265

RMK:	RAN89_11135

RLH:	MIZ03_2327

POL:

Bpro_3387

PNA:

Pnap_2725

ACK:	C380_22780

ACRA:

BSY15_1230

ACIO:

EAG14_01170

ARAD:

KI609_21515

ACIQ:

ACDW_40190

ACIV:

EXV95_14995

AMON:

H9L24_11780

VEI:	Veis_1368 Veis_1485

DAC:

Daci_1352

DEL:	DelCs14_5161

DTS:	BI380_17925

DLA:	I6G47_17780

VAP:	Vapar_5693

VPE:	Varpa_1917

VPD:	VAPA_2c01950(vanA)

VAA:	AX767_16425

VBO:	CKY39_11895

VAM:	C4F17_00155

VAZ:	H7F35_22750

VAV:	IG196_09765

CTT:	CtCNB1_4192

CTES:

O987_24985

CRJ:	QMY55_20615

LIM:	L103DPR2_01485(tsaM1)

LIH:	L63ED372_00786(tsaM1_1)

HYR:	BSY239_1252 BSY239_2692

HYB:

Q5W_09005

HYL:	LPB072_17960

HPSE:

HPF_20485(tsaM2)

HYN:	F9K07_04905

HTN:	KI616_20740

HCZ:	G9Q37_03425 G9Q37_13935

HYY:	JNX00_10565

CBAA:

SRAA_2261

CBAB:

SMCB_0812

HGR:	DW355_04310

STHM:

IS481_07625

SNN:	EWH46_19805

SMIO:

CATMQ487_47070(vanA)

ATER:

MW290_13485

LCH:

Lcho_1335

HSE:	Hsero_3992(vanA)

HSZ:	ACP92_19930

HRB:	Hrubri_3944(vanA)

HEE:	hmeg3_01210

HHF:	E2K99_20795

HFR:	G5S34_21680

HEW:	HBDW_40810

MASW:

AM586_11615

MLIR:

LPB04_14690

MALI:

EYF70_16035

MUM:	FCL38_02380

MFLA:

GO485_27725

PLUE:

EWM63_00310

DZO:	SR858_08485

TMJ:	P0M04_23915

GLC:	JQN73_01740

AZR:	CJ010_15660

TCL:

Tchl_3188

TAK:	Tharo_2222

AGC:	BSY240_3610

RROS:

D4A92_02925

PDES:

FE840_020065

BJA:	blr2390(vanA)

BJU:	BJ6T_74460

BJP:	RN69_36110

BRA:

BRADO1867

BBT:

BBta_2184

BRS:	S23_56180(vanA)

AOL:

S58_56900

BRC:	BCCGELA001_27935 BCCGELA001_31305

BRAD:

BF49_2414

BIC:	LMTR13_06900

BRO:	BRAD285_5428(vanA)

BRK:	CWS35_13720

BOT:	CIT37_00690

BRQ:	CIT40_06450

BGQ:	X265_29230

BGZ:	XH91_27175

BSYM:

CIT39_05220

BBET:

F8237_07970

BARH:

WN72_12040

BVZ:	BRAD3257_6671(vanA)

BEL:	BE61_29140(vanA) BE61_81100(vanA)

BDG:	LPJ38_33955

BSEP:

HAP48_0007260

BQB:	J4P68_0028645

BSEI:

KMZ68_01225

BBAN:

J4G43_013150

BCAN:

BcanWSM471_10990

BAUT:

QA635_10045 QA635_34875

BBRA:

QA636_09125 QA636_36405

BGK:	IC762_08185

BCOU:

IC761_27405

BPAH:

QA639_35115

BXN:	I3J27_08065

BROS:

QUH67_08280

BRAZ:

LRP30_09785

BOY:	LQG66_14775

BRAY:

ML401_07180

RPA:	TX73_018755

RPB:

RPB_1908

RPT:

Rpal_4139

RPX:	Rpdx1_1730

RBK:	E0H22_08395

RHOR:

ONR75_28135

TRB:	HB776_01805

BOS:	BSY19_2758

XDI:	EZH22_08910

MNO:

Mnod_7105

META:

Y590_19195

MAQU:

Maq22A_c10720(hcaE)

MEE:	DA075_24625

MTEA:

DK419_21100

MIND:

mvi_02480(vanA)

MOC:	BB934_00220

MTEZ:

HPT29_013210

PEM:	OF122_05180

DEI:	C4375_09775

DSAL:

K1X15_17485

DEVO:

H4N61_07385

DOY:	JI749_05685

MHAR:

L1P08_06815

BRN:	D1F64_22325

CCR:

CC_2393

CCS:	CCNA_02476(vanA)

CAK:

Caul_1137

CSE:

Cseg_2340

BRF:	E4M01_02225

CON:	TQ29_16445

PPRU:

FDP22_15025

PGV:	SL003B_2863

GYJ:	AADW23_06585

NPP:	PP1Y_Mpl10434

NPN:	JI59_20890

NOL:	NSDW_32770

SINA:

KNJ79_13900

SSAN:

NX02_17555

SPAN:

AWL63_04695

SKR:	BRX40_12235

SPII:

G7077_01585

SSUA:

FPZ54_06190

SBIN:

SBA_ch2_5410(vanA_3) SBA_ch2_5500(vanA_4)

SHAN:

PPZ50_15715

SPHB:

EP837_03492(vanA)

SPHY:

CHN51_00900

BLAS:

BSY18_3501

BFW:	B5J99_08300

SPHS:

ETR14_08005

RGI:	RGI145_21085

ROS:	CTJ15_00925

RMUC:

FOB66_01760

ACIM:

GT370_03550

AZL:	AZL_b03640(vanA)

AZZ:	DEW08_26970

AZS:	E6C72_25000

NAO:	Y958_28550

NCB:	C0V82_19015

FER:	FNB15_12425

THAI:

IT893_15360

TTB:	MACH01_30180(vanA)

GSO:	PH603_12895

AVP:	AVENP_2155(ligM)

PARH:

I5S86_11620

MSEI:

MSEDJ_44440

CGL:	Cgl2383(Cgl2383)

CGB:	cg2616(vanA)

CGU:	WA5_2300(VanA)

CGT:

cgR_2265

CGS:	C624_11570

CGG:	C629_11580

CGM:	cgp_2616(vanA)

CGJ:

AR0_11410

CGQ:	CGLAR1_11260

CGX:	SB89_11065

CEF:

CE0634

CVA:	CVAR_2696(vanA)

CHN:	A605_00720

CCJZ:

ccrud_10600

CSUR:

N24_2454(vanA)

CCOE:

CETAM_07705(tsaM1)

CPOY:

GP475_09050

BFV:	C628_11540

RPY:	Y013_13470

RRZ:	CS378_17620

RHQ:	IM25_04815

RRT:	4535765_04621

ROZ:	CBI38_25030

GPO:	GPOL_c13810

GOI:	LK459_09205

SHY:

SHJG_0203

MTS:

MTES_2133

MIH:	BJP65_08820

MOO:	BWL13_01875(tsaM1)

AUM:	AURMO_01281

SALC:

C2138_12115

SALD:

FVA74_12750

LRP:	MUN76_08415

ARR:	ARUE_c24110(vanA)

ACRY:

AC20117_04925

ARTP:

E5206_02400

ASUF:

MNQ99_02465

ACAO:

NF551_01670

AKO:	N9A08_14415

GLU:	F0M17_01970

GNC:	QQS42_02090

KPL:	KPaMU14_06260

KRS:	EQG70_02565

CCYC:

SCMU_37970

NAE:	BHE16_09630

PAEY:

KUF55_17160

NAKA:

H7F38_22045

GOB:

Gobs_2826

SVI:	Svir_26080

PBRO:

HOP40_32900

PSDS:

K1T35_16975

ACTY:

OG774_04295

JTL:	M6D93_11230

MARM:

YQ22_04915

MARB:

CJ263_16380

» show all

Reference

1 [PMID:3170489]

Authors

Brunel F, Davison J

Title

Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase.

Journal

J Bacteriol 170:4924-30 (1988)
DOI:10.1128/JB.170.10.4924-4930.1988

Sequence

[ag:AAA26019]

Reference

2 [PMID:9098058]

Authors

Priefert H, Rabenhorst J, Steinbuchel A

Title

Molecular characterization of genes of Pseudomonas sp. strain HR199 involved in bioconversion of vanillin to protocatechuate.

Journal

J Bacteriol 179:2595-607 (1997)
DOI:10.1128/JB.179.8.2595-2607.1997

Sequence

[ag:CAA72287]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.82

IUBMB Enzyme Nomenclature:

1.14.13.82

ExPASy - ENZYME nomenclature database:

1.14.13.82

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.13.82

BRENDA, the Enzyme Database:

1.14.13.82

CAS:	39307-11-4

DBGET integrated database retrieval system