KEGG   ENZYME: 1.14.13.82
Entry
EC 1.14.13.82               Enzyme                                 
Name
vanillate monooxygenase;
4-hydroxy-3-methoxybenzoate demethylase;
vanillate demethylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
vanillate:oxygen oxidoreductase (demethylating)
Reaction(IUBMB)
vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde [RN:R05274]
Reaction(KEGG)
R05274
Substrate
vanillate [CPD:C06672];
O2 [CPD:C00007];
NADH [CPD:C00004];
H+ [CPD:C00080]
Product
3,4-dihydroxybenzoate [CPD:C00230];
NAD+ [CPD:C00003];
H2O [CPD:C00001];
formaldehyde [CPD:C00067]
Comment
Forms part of the vanillin degradation pathway in Arthrobacter sp.
History
EC 1.14.13.82 created 2000 as EC 1.2.3.12, transferred 2003 to EC 1.14.13.82
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K03862  vanillate monooxygenase
Genes
PAEPA4904(vanA)
PAEVN297_5074
PAEIN296_5074
PAUPA14_64800(vanA)
PAPPSPA7_5628(vanA)
PAGPLES_52901(vanA)
PAFPAM18_5015(vanA)
PNCNCGM2_0620(vanA)
PAEBNCGM1900_5646(vanA)
PDKPADK2_26010
PSGG655_25860
PRPM062_25870
PAEPPA1S_26620
PAERPA1R_gp2826
PAEMU769_26885
PAELT223_27080
PAESSCV20265_5564
PAEUBN889_03196(vanA)
PAEGAI22_07375
PAECM802_5072
PAEOM801_4939
PREPCA10_51390(vanA)
PCQPcP3B5_56530(tsaM1_3)
PPUPP_3736(vanA)
PPFPput_2027
PPGPputGB1_2170
PPWPputW619_2045
PPBPPUBIRD1_2026
PPIYSA_09409
PPXT1E_1717(vanA)
PPUTL483_19445
PPUNPP4_20850(vanA)
PFVPsefu_4050
PORAPT59_07750
PSTPSPTO_2904(vanA)
PSBPsyr_2708
PSYRN018_13725
PSPPSPPH_2475(vanA)
PSAVPSA3335_15475
PAMGBKM19_016235
PAVLBKM03_14475
PVDCFBP1590__2812(vanA)
PCABJGS08_12860
PFLPFL_3471(vanA)
PPRCPFLCHA0_c35050(vanA)
PPROPPC_3614
PFEPSF113_2802
PFSPFLU_3293
PPZH045_05725
PTVAA957_00695
PCGAXG94_01515
PVRPverR02_12230
PSTTCH92_04375
PLULFOB45_22220
PBAPSEBR_a2987
PBCCD58_14310
PDRH681_21940
PSVPVLB_10605
PSKU771_11805
PCHEY04_12300
PCZPCL1606_35110
PCPJM49_17570
PFZAV641_10035
PSESPSCI_1617
PSEMTO66_13045
PPSYAOC04_06905
PSOSPOS17_3599
PPSLBJP27_22580
PUMHGP31_09950
POJPtoMrB4_29730(vanA)
PPSHG5J76_09050 G5J76_17005
PVKEPZ47_14290
PEZHWQ56_13535
PBZGN234_02485
PMAOPMYSY11_2124(vanA)
PASGKSS96_11865
PALVKSS97_15845
PZAHU749_016095
ACBA1S_1116
ABYABAYE2623(vanA)
ABNAB57_1200
ABBABBFA_02396(tsaM1_1)
ABABBJAB0715_01306
ABAZP795_11745
ABAUIX87_05265
ABAAIX88_07830
ABALABLAC_19570
ACCBDGL_000396(vanA)
ANORR32_12590
ALCOTEC02_13335
ACALBUM88_05435
ASEII8T81_13365
ACDAOLE_13720
ACIACIAD0980(vanA)
AEIAOY20_07280
ACWA0J50_11960
AHLAHTJS_03680
ASOLBEN76_08720
AGUAS4_34520(vanA)
AUGURS_1853
ATNFM020_02805
PATPatl_0844
AAWAVL56_08685
ALRDS731_00575
CSACsal_0344
HELHELO_3940(vanA)
HAMHALO0093
HMDCTT34_17620
HTTHZS52_05285
HCAMI4484_18900
HSXHNO51_18955
HSVHNO53_19215
HTXEKK97_21215
MMWMmwyl1_1296
MMEMarme_1172
MPCMar181_2609
MPRIMP3633_2749
MARDIBG28_05285
MFOIJSY38_04510
MPRFJ8N69_01790
GSNYC6258_03914
NBLGJV52_04080
VITADP71_28920
RSORSp0222(vanA)
RSLRPSI07_mp0173(vanA)
RSNRSPO_m00210(vanA)
RSMCMR15_mp10192(vanA)
RSEF504_3661
RSYRSUY_35550(tsaM1)
RPUCDC45_18725
CBWRR42_s2469
CCUPBKK81_20160
CUPBKK80_21645
CUUBKK79_26810
BVIBcep1808_3498
BVEAK36_3260
BURBcep18194_B0420
BCNBcen_3124
BCHBcen2424_5243
BCMBcenmc03_5029
BCJBCAM2436(vanA)
BCENDM39_5402
BCEWDM40_3099
BCEOI35_6336(vanA)
BAMBamb_4591
BACBamMC406_5124
BMJBMULJ_05311(vanA)
BMUBmul_3214
BMKDM80_4594
BMULNP80_3339
BCTGEM_3253
BCEDDM42_5545
BCEPAPZ15_22685
BDLAK34_5017
BPYRABD05_21690
BCONNL30_16005
BDFWI26_25000
BLATWK25_28075
BTEIWS51_09170
BSEMWJ12_32220
BPSLWS57_01935
BMECWJ16_30710
BSTLBBJ41_26115
BANNJFN94_16415
BGLbglu_2g14550
BGUKS03_4484
BUKMYA_3124
BURKDM992_23760
BGEBC1002_3291
PSPWBJG93_19170
PCAFDSC91_002445
PACPFAZ97_28965
PPKU875_22470
PPNODA70_15150
PPNMLV28_15530
PRBX636_11695
PPULRO07_09575
PSPUNA29_05490
PAPISG18_10075
PVEUC34_01360
POXMB84_05695
PFGAB870_12615
PNRAT302_09260
PANDDRB87_01225
PFIBPI93_001180
PUDG9Q38_04025 G9Q38_10650 G9Q38_11445 G9Q38_13355
CDNBN940_08501
PHNPAEH1_08445
PIGEGT29_00385
RFRRfer_0280
RHYRD110_11775
RHFEUB48_13000
PNAPnap_2725
ACKC380_22780
ACRABSY15_1230
ACIOEAG14_01170
AMONH9L24_11780
ARADKI609_21515
VEIVeis_1485
DACDaci_1352
DELDelCs14_5161
DTSBI380_17925
DLAI6G47_17780
VAPVapar_5693
VPDVAPA_2c01950(vanA)
CTTCtCNB1_4192
CTESO987_24985
LIML103DPR2_01485(tsaM1)
LIHL63ED372_00786(tsaM1_1)
HYRBSY239_1252 BSY239_2692
HYLLPB072_17960
HPSEHPF_20485(tsaM2)
HYNF9K07_04905
HTNKI616_20740
CBAASRAA_2261
CBABSMCB_0812
STHMIS481_07625
HSEHsero_3992(vanA)
HSZACP92_19930
HRBHrubri_3944(vanA)
HEEhmeg3_01210
HHFE2K99_20795
HFRG5S34_21680
MASWAM586_11615
MALIEYF70_16035
MUMFCL38_02380
MFLAGO485_27725
GLCJQN73_01740
LCHLcho_1335
SNNEWH46_19805
AZRCJ010_15660
TCLTchl_3188
TAKTharo_2222
AVPAVENP_2155(ligM)
CCRCC_2393
CCSCCNA_02476(vanA)
CSECseg_2340
SPANAWL63_04695
SECHB18_11340
BLASBSY18_3501
BFWB5J99_08300
PARHI5S86_11620
 » show all
Reference
1  [PMID:3170489]
  Authors
Brunel F, Davison J
  Title
Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase.
  Journal
J Bacteriol 170:4924-30 (1988)
DOI:10.1128/JB.170.10.4924-4930.1988
  Sequence
Reference
2  [PMID:9098058]
  Authors
Priefert H, Rabenhorst J, Steinbuchel A
  Title
Molecular characterization of genes of Pseudomonas sp. strain HR199 involved in bioconversion of vanillin to protocatechuate.
  Journal
J Bacteriol 179:2595-607 (1997)
DOI:10.1128/JB.179.8.2595-2607.1997
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.82
IUBMB Enzyme Nomenclature: 1.14.13.82
ExPASy - ENZYME nomenclature database: 1.14.13.82
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.82
BRENDA, the Enzyme Database: 1.14.13.82
CAS: 39307-11-4

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