KEGG   ENZYME: 1.14.13.83Help
Entry
EC 1.14.13.83               Enzyme                                 

Name
precorrin-3B synthase;
precorrin-3X synthase;
CobG
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
precorrin-3A,NADH:oxygen oxidoreductase (20-hydroxylating)
Reaction(IUBMB)
precorrin-3A + NADH + H+ + O2 = precorrin-3B + NAD+ + H2O [RN:R05217]
Reaction(KEGG)
Substrate
precorrin 3A [CPD:C05772];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
precorrin 3B [CPD:C06406];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
An iron-sulfur protein. An oxygen atom from dioxygen is incorporated into the macrocycle at C-20. In the aerobic cobalamin biosythesis pathway, four enzymes are involved in the conversion of precorrin-3A to precorrin-6A. The first of the four steps is carried out by EC 1.14.13.83, precorrin-3B synthase (CobG), yielding precorrin-3B as the product. This is followed by three methylation reactions, which introduce a methyl group at C-17 (CobJ; EC 2.1.1.131), C-11 (CobM; EC 2.1.1.133) and C-1 (CobF; EC 2.1.1.152) of the macrocycle, giving rise to precorrin-4, precorrin-5 and precorrin-6A, respectively.
History
EC 1.14.13.83 created 2004
Pathway
ec00860  Porphyrin and chlorophyll metabolism
ec01100  Metabolic pathways
Orthology
K02229  precorrin-3B synthase
Genes
FAU: Fraau_1285
PAE: PA2906
PAEV: N297_3009(cobG)
PAEI: N296_3009(cobG)
PAU: PA14_26485
PAP: PSPA7_2248(cobG)
PAG: PLES_21581
PAF: PAM18_2056
PNC: NCGM2_4016
PAEB: NCGM1900_3405
PAEP: PA1S_10880
PAEM: U769_10420
PAEL: T223_10890
PAEG: AI22_22990
PAEC: M802_3006(cobG)
PAEO: M801_2874(cobG)
PMY: Pmen_4571
PMK: MDS_4903
PRE: PCA10_06440(cobG)
PPSE: BN5_4376(nirA)
PCQ: PcP3B5_08200(sir_1)
PPU: PP_4829(cobG)
PPF: Pput_4707
PPT: PPS_4673
PPI: YSA_03772
PPX: T1E_3711
PPUH: B479_23600
PPUT: L483_29295
PPUN: PP4_48990(cobG)
PPUD: DW66_5064
PMON: X969_23050
PMOT: X970_22685
PSB: Psyr_4417
PSYR: N018_22885
PAMG: BKM19_025865(cobG)
PAVL: BKM03_25620(cobG)
PPRC: PFLCHA0_c06640(nirA)
PPRO: PPC_0668
PFS: PFLU_0604
PFE: PSF113_0640(cobG)
PFC: PflA506_0584(cobG)
PFW: PF1751_v1c05660(cobG)
PFB: VO64_3679
PMAN: OU5_2849
PEN: PSEEN4871(cobG)
PPUU: PputUW4_00538(cobG)
PKC: PKB_0655
PSES: PSCI_2413
PSEM: TO66_03125
PSOS: POS17_0648
PANR: A7J50_0586
PSET: THL1_740
PSIL: PMA3_02495
ACX: Achr_f740(cobG)
TKM: TK90_0801
TVR: TVD_04725
HEL: HELO_1833(cobG)
HAM: HALO2053
EME: CEM_066(cobG)
BMA: BMA1161
BML: BMA10229_A0264(cobG)
BMN: BMA10247_0896(cobG)
BMAL: DM55_2997(cobG)
BMAE: DM78_1463(cobG)
BMAQ: DM76_2979(cobG)
BMAI: DM57_62
BMAF: DM51_890(cobG)
BMAZ: BM44_2182(cobG)
BMAB: BM45_1746(cobG)
BPS: BPSL1759
BPM: BURPS1710b_2113(cobG)
BPL: BURPS1106A_1967(cobG)
BPD: BURPS668_1950(cobG)
BPR: GBP346_A1991(cobG)
BPSE: BDL_283(cobG)
BPSM: BBQ_1620(cobG)
BPSU: BBN_1745(cobG)
BPSD: BBX_2220(cobG)
BPK: BBK_3245(cobG)
BPSH: DR55_2853(cobG)
BPSA: BBU_456(cobG)
BPSO: X996_2474(cobG)
BUT: X994_923(cobG)
BTE: BTH_I2398
BTJ: BTJ_834(cobG)
BTZ: BTL_2077(cobG)
BTD: BTI_2041(cobG)
BTV: BTHA_2276(cobG)
BTHE: BTN_2690(cobG)
BTHM: BTRA_2356(cobG)
BTHA: DR62_2838
BTHL: BG87_2298(cobG)
BOK: DM82_1296(cobG)
BOC: BG90_3396(cobG)
BVE: AK36_2237(cobG)
BCN: Bcen_1194
BCJ: BCAL1726
BCEN: DM39_1638(cobG)
BCEW: DM40_2297(cobG)
BCEO: I35_1639
BAM: Bamb_1574
BMU: Bmul_1568
BMJ: BMULJ_01676(cobG)
BMK: DM80_3324(cobG)
BMUL: NP80_1764(cobG)
BCT: GEM_1742
BCED: DM42_23(cobG)
BDL: AK34_1437(cobG)
BCON: NL30_14715
BUB: BW23_49(cobG)
BLAT: WK25_08190
BTEI: WS51_18885
BSEM: WJ12_08285
BPSL: WS57_26840
BMEC: WJ16_08390
BSTG: WT74_08620
BGU: KS03_2696(cobG)
BGO: BM43_3281(cobG)
BUL: BW21_2106(cobG)
BGP: BGL_1c18550(cobG)
BXE: Bxe_B1245
BXB: DR64_6560(cobG)
BPH: Bphy_3143
BFN: OI25_6988(cobG)
PARB: CJU94_22965(cobG)
PHS: C2L64_26525(cobG)
PTER: C2L65_21860(cobG)
PGP: CUJ91_23005(cobG)
PLG: NCTC10937_00830(sir_1)
DAC: Daci_5851
CTES: O987_11765
MLO: mlr1378
AMIH: CO731_02904(sir_2)
SME: SMc03193(cobG)
SMX: SM11_chr3096(cobG)
SMI: BN406_02780(cobG)
SMEL: SM2011_c03193(cobG)
SMER: DU99_16110
SMD: Smed_2818
RHI: NGR_c29810(cobG)
SFH: SFHH103_02988(cobG)
SFD: USDA257_c53970(cobG)
SIX: BSY16_3269(cobG)
SAME: SAMCFNEI73_Ch3353(cobG)
EAD: OV14_0449(cobG)
ATU: Atu2803(cobG)
ARA: Arad_9596(cobG)
ATF: Ach5_26870(cobG)
AGR: AGROH133_09256(cobG)
ARO: B0909_26060(cobG)
RET: RHE_PE00455(cobG)
REC: RHECIAT_PA0000378(cobG)
REL: REMIM1_PD00478(cobG)
REP: IE4803_PA00414(cobG)
REI: IE4771_PC00427(cobG)
RLE: pRL110631(cobG)
RLG: Rleg_7174
RTR: RTCIAT899_PC06990(cobG)
RIR: BN877_I2891(cobG)
RHL: LPU83_pLPU83d0187(cobG)
RGA: RGR602_PC01216(cobG)
RHN: AMJ98_PD00464(cobG)
RPHA: AMC79_PB00387(cobG)
RHT: NT26_3123(cobG)
RHX: AMK02_PE00471(cobG)
RHK: Kim5_PB00447(cobG)
REZ: AMJ99_PC00465(cobG)
NEO: CYG48_18570(cobG)
NEN: NCHU2750_36450(cobG)
SHZ: shn_19590
BME: BMEI0715
BMEL: DK63_712(cobG)
BMI: BMEA_A1333(cobG)
BMZ: BM28_A1297(cobG)
BMEE: DK62_142(cobG)
BMF: BAB1_1304
BABO: DK55_1281(cobG)
BABR: DO74_609(cobG)
BABT: DK49_1038(cobG)
BABB: DK48_839(cobG)
BABU: DK53_1265(cobG)
BABS: DK51_195(cobG)
BABC: DO78_1186(cobG)
BMS: BR1286
BSUI: BSSP1_I0407(cobG)
BSUP: BSPT1_I0414(cobG)
BSUV: BSPT2_I0409(cobG)
BSUC: BSSP2_I1312(cobG)
BMT: BSUIS_A1336(cobG)
BSZ: DK67_1012(cobG)
BOV: BOV_1249(cobG)
BCS: BCAN_A1309(cobG)
BCAR: DK60_1325(cobG)
BCAS: DA85_06170
BMR: BMI_I1299(cobG)
BPP: BPI_I1338(cobG)
BPV: DK65_94(cobG)
BVL: BF3285c1_2096(cobG)
OAN: Oant_1899
OAH: DR92_1387(cobG)
OCH: CES85_1434(cobG)
BJA: blr3264
BRA: BRADO4906
BBT: BBta_3145
BRS: S23_47400
AOL: S58_27840(cobG)
BRAD: BF49_1434
BRO: BRAD285_2330(cobG)
BRK: CWS35_19605(cobG)
BOT: CIT37_37240(cobG)
BRQ: CIT40_10805(cobG)
BOS: BSY19_876(cobG)
XAU: Xaut_3285
AZC: AZC_3936(cobG)
SNO: Snov_2570
MEX: Mext_1438
MEA: Mex_1p1327(cobG)
MDI: METDI2099(cobG)
MCH: Mchl_1713
MPO: Mpop_1435
MET: M446_2439
MNO: Mnod_2150
MOR: MOC_3496
META: Y590_06690
MAQU: Maq22A_c09430(cysI)
METD: C0214_08165(cobG)
BID: Bind_3508
MSL: Msil_3267
HDN: Hden_2698
PHL: KKY_929
DEI: C4375_01915(cobG)
MSC: BN69_3121
MCG: GL4_0404
HDI: HDIA_3058(cobG)
SIL: SPO2864
JAN: Jann_2930
RDE: RD1_3826(cobG)
RLI: RLO149_c006180(cobG)
PDE: Pden_2533
PZH: CX676_19960(cobG)
PARO: CUV01_00705(cobG)
PGD: Gal_02676
PHQ: D1820_17190(cobG)
CID: P73_0491
MALG: MALG_01209
SULZ: C1J03_01430(cobG)
NAR: Saro_0341
SPHP: LH20_21945
STAX: MC45_16195
SJP: SJA_C2-03100(cobG)
SPMI: K663_18391
SPHB: EP837_03434(cobG)
SINB: SIDU_11835
SPHT: K426_22459
GOH: B932_2913
AMV: ACMV_P1_00620(cobG)
GXY: GLX_19990
GXL: H845_2986
KEU: S101446_02477(cobG)
APT: APA01_14560(cobG)
APW: APA42C_14560(cobG)
APF: APA03_14560(cobG)
APU: APA07_14560(cobG)
APG: APA12_14560(cobG)
APQ: APA22_14560(cobG)
APX: APA26_14560(cobG)
APZ: APA32_14560(cobG)
APK: APA386B_318(CobG)
ASZ: ASN_3532(cobG)
ATO: CIW82_13855(cobG)
ROS: CTJ15_10580(cobG)
AZL: AZL_d03290(cobG)
ALI: AZOLI_p40247(cobG)
AZT: TSH58p_24040(cobG)
AZM: DM194_24375(cobG)
NAO: Y958_29295(cobG)
MTU: Rv2064(cobG)
MTV: RVBD_2064
MTC: MT2124
MRA: MRA_2078(cobG)
MTUR: CFBS_2185(cobG)
MTO: MTCTRI2_2102(cobG)
MTD: UDA_2064(cobG)
MTN: ERDMAN_2279(cobG)
MTUE: J114_11050
MTUH: I917_14550
MTUL: TBHG_02022
MTUT: HKBT1_2179(cobG)
MTUU: HKBT2_2180(cobG)
MTQ: HKBS1_2184(cobG)
MBO: BQ2027_MB2090(cobG)
MBB: BCG_2083(cobG)
MBT: JTY_2077(cobG)
MBM: BCGMEX_2067(cobG)
MAF: MAF_20790(cobG)
MMIC: RN08_2288
MCE: MCAN_20871(cobG)
MCQ: BN44_40356(cobG)
MCV: BN43_31257(cobG)
MCX: BN42_30375(cobG)
MCZ: BN45_50371(cobG)
MPA: MAP_1810(cobG)
MAO: MAP4_2018
MAVI: RC58_10055
MAVU: RE97_10065
MIT: OCO_24280
MIA: OCU_24150
MID: MIP_03355
MYO: OEM_22730
MIR: OCQ_22820
MMAL: CKJ54_11005(cobG)
MUL: MUL_2288
MMC: Mmcs_2489
MKM: Mkms_2534
MJL: Mjls_2526
MMI: MMAR_3044
MMM: W7S_11715
MHAD: B586_10815
MSM: MSMEG_3871(cobG)
MSG: MSMEI_3781(cobG)
MVA: Mvan_3429
MGI: Mflv_3106
MFT: XA26_35740(cobG)
MVQ: MYVA_3340
MAB: MAB_2200c
MABB: MASS_2127
MCHE: BB28_11065
MSTE: MSTE_02139
ASD: AS9A_2041
CDI: DIP1231
CDIP: ERS451417_01231(cobG)
CAR: cauri_1273(cobG)
CKP: ckrop_0898(cobG)
CPU: cpfrc_01024(cobG)
CPL: Cp3995_1043(cobG)
CPG: Cp316_1064(cobG)
CPP: CpP54B96_1039(cobG)
CPK: Cp1002_1020(cobG)
CPQ: CpC231_1019(cobG)
CPX: CpI19_1025(cobG)
CPZ: CpPAT10_1019(cobG)
COR: Cp267_1068(cobG)
COP: Cp31_1029(cobG)
COD: Cp106_1002(cobG)
COS: Cp4202_1012(cobG)
COI: CpCIP5297_1037(cobG)
COE: Cp258_1034(cobG)
COU: Cp162_1018(cobG)
CPSE: CPTA_01591
CPSU: CPTB_02173
CPSF: CPTC_01581
CUL: CULC22_01094(cobG)
CUC: CULC809_01079(cobG)
CUE: CULC0102_1201(cobG)
CUN: Cul210932_1133(cobG)
CUS: CulFRC11_1103(cobG)
CUQ: Cul210931_1085(cobG)
CUZ: Cul05146_1159(cobG)
CUJ: CUL131002_1117(cobG)
COA: DR71_80(cobG)
CMV: CMUST_07935(cobG)
CSP: WM42_0083
CPHO: CPHO_06140
CAMG: CAMM_06675
CMIN: NCTC10288_01251(cobG)
NFA: NFA_31400
NCY: NOCYR_3312(cobG)
NSR: NS506_05033(cobG)
NTP: CRH09_17450(cobG)
RER: RER_31420(cobG)
REY: O5Y_14425
ROP: ROP_02920(cobG)
REQ: REQ_23580(cobG)
RHB: NY08_990
RQI: C1M55_15675(cobG)
GBR: Gbro_2481
GPO: GPOL_c24150(cobG)
GOR: KTR9_2390
GOC: CXX93_12410(cobG)
GIT: C6V83_11340(cobG)
TPR: Tpau_2192
SALB: XNR_4668
SGR: SGR_3824
SGB: WQO_16615
SCB: SCAB_9781(cobG)
SFA: Sfla_3029
SBH: SBI_01765
SVE: SVEN_3517
STRP: F750_3737(cobG)
SFI: SFUL_3820
SALU: DC74_4103
SALL: SAZ_21870
STRE: GZL_04792
SLD: T261_4279
STRM: M444_26935
SPRI: SPRI_5139
SLAU: SLA_3556
SALJ: SMD11_0881(cobG)
SLX: SLAV_09390(sir1)
SFK: KY5_6082
KSK: KSE_64810(cobG)
LMOI: VV02_21340
MPH: MLP_03490(cobG)
NDK: I601_3837
KFL: Kfla_5082
PSIM: KR76_17040
TFU: Tfu_0318
NDA: Ndas_1626
NAL: B005_0636
SRO: Sros_5592
FAL: FRAAL2331(cobG)
NML: Namu_0151
GOB: Gobs_0576
BSD: BLASA_1265(cobG)
MMAR: MODMU_0657(cobG)
SEN: SACE_5953
AMD: AMED_7063(cobG)
AMN: RAM_36240
AMM: AMES_6955(cobG)
AMZ: B737_6955(cobG)
AOI: AORI_1667(cobG)
AMQ: AMETH_5466(cobG)
PDX: Psed_3022
PSEA: WY02_28250
PSEH: XF36_14705
AMI: Amir_1783
SESP: BN6_25830
ALO: CRK57394
MIL: ML5_4312
ASE: ACPL_6899(cobG)
ACTN: L083_6534
AFS: AFR_33980
ACTS: ACWT_6768
TBI: Tbis_2494
AEY: CDG81_07520(cobG)
ABAS: ACPOL_5051
 » show all
Taxonomy
Reference
1  [PMID:8226690]
  Authors
Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F.
  Title
Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas denitrificans.
  Journal
J Bacteriol 175:7430-40 (1993)
DOI:10.1128/JB.175.22.7430-7440.1993
Reference
2  [PMID:8405386]
  Authors
Scott AI, Roessner CA, Stolowich NJ, Spencer JB, Min C, Ozaki SI.
  Title
Biosynthesis of vitamin B12. Discovery of the enzymes for oxidative ring contraction and insertion of the fourth methyl group.
  Journal
FEBS Lett 331:105-8 (1993)
DOI:10.1016/0014-5793(93)80306-F
Reference
3  [PMID:12195810]
  Authors
Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC.
  Title
The biosynthesis of adenosylcobalamin (vitamin B12).
  Journal
Nat Prod Rep 19:390-412 (2002)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.83
IUBMB Enzyme Nomenclature: 1.14.13.83
ExPASy - ENZYME nomenclature database: 1.14.13.83
BRENDA, the Enzyme Database: 1.14.13.83
CAS: 152787-63-8

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