KEGG   ENZYME: 1.14.13.93Help
Entry
EC 1.14.13.93               Enzyme                                 

Name
(+)-abscisic acid 8'-hydroxylase;
(+)-ABA 8'-hydroxylase;
ABA 8'-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
abscisate,NADPH:oxygen oxidoreductase (8'-hydroxylating)
Reaction(IUBMB)
(+)-abscisate + NADPH + H+ + O2 = 8'-hydroxyabscisate + NADP+ + H2O [RN:R07202]
Reaction(KEGG)
Substrate
(+)-abscisate;
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
8'-hydroxyabscisate [CPD:C15514];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). Catalyses the first step in the oxidative degradation of abscisic acid and is considered to be the pivotal enzyme in controlling the rate of degradation of this plant hormone [1]. CO inhibits the reaction, but its effects can be reversed by the presence of blue light [1]. The 8'-hydroxyabscisate formed can be converted into (-)-phaseic acid, most probably spontaneously. Other enzymes involved in the abscisic-acid biosynthesis pathway are EC 1.1.1.288 (xanthoxin dehydrogenase), EC 1.2.3.14 (abscisic-aldehyde oxidase) and EC 1.13.11.51 (9-cis-epoxycarotenoid dioxygenase).
History
EC 1.14.13.93 created 2005
Pathway
ec00906  Carotenoid biosynthesis
Orthology
K09843  (+)-abscisic acid 8'-hydroxylase
Genes
RSS: 109441020
ATH: AT2G29090(CYP707A2) AT3G19270(CYP707A4) AT4G19230(CYP707A1) AT5G45340(CYP707A3)
ALY: ARALYDRAFT_479442 ARALYDRAFT_481828 ARALYDRAFT_492925 ARALYDRAFT_683506
CRB: 17874776 17880652 17887641 17892766
CSAT: 104700603 104718390 104723117 104724884 104733490 104746312 104749974 104760380 104765790 104771755 104784217 104786786
EUS: EUTSA_v10000876mg EUTSA_v10016581mg EUTSA_v10020656mg EUTSA_v10025059mg
BRP: 103827806 103839129 103849374 103857276 103859803 103861043 103869474
BNA: 106361235 106373335 106374933 106379426 106386394 106391221 106391222 106395068 106416491 106416608 106419785 106425473 106427439 106428338 106444094 106446905 111197871 111204767 111206585 111208232 111209689 111215074
BOE: 106292604 106301431 106304342 106319075 106322586 106332542 106336684 106337621 106344046
THJ: 104798530 104798797 104800678 104806199 104811149 104819469
CIT: 102611561(CYP707A4) 102613470(CYP707A1) 102616423(CYP707A3) 102627021(CYP707A2)
LJA: Lj0g3v0285509.1(Lj0g3v0285509.1) Lj0g3v0332329.1(Lj0g3v0332329.1) Lj0g3v0340109.1(Lj0g3v0340109.1) Lj2g3v1550580.1(Lj2g3v1550580.1) Lj5g3v0290410.1(Lj5g3v0290410.1)
SLY: 100136887(CYP707A1) 101249565(CYP707A2) 101254720(SlCYP707A3) 101266767
DOSA: Os02t0703600-01(Os02g0703600) Os08t0472800-01(Os08g0472800) Os09t0457100-01(Os09g0457100)
ATS: 109739218(LOC109739218) 109763237(LOC109763237)
PPP: 112276337
MNG: MNEG_7206
 » show all
Taxonomy
Reference
1
  Authors
Cutler, A.J., Squires, T.M., Loewen, M.K. and Balsevich, J.J.
  Title
Induction of (+)-abscisic acid 8' hydroxylase by (+)-abscisic acid in cultured maize cells.
  Journal
J Exp Bot 48:1787-1795 (1997)
Reference
2  [PMID:9808729]
  Authors
Krochko JE, Abrams GD, Loewen MK, Abrams SR, Cutler AJ.
  Title
(+)-Abscisic acid 8'-hydroxylase is a cytochrome P450 monooxygenase
  Journal
Plant Physiol 118:849-60 (1998)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.93
IUBMB Enzyme Nomenclature: 1.14.13.93
ExPASy - ENZYME nomenclature database: 1.14.13.93
BRENDA, the Enzyme Database: 1.14.13.93
CAS: 153190-37-5

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