KEGG   ENZYME: 1.14.14.110
Entry
EC 1.14.14.110              Enzyme                                 

Name
2-hydroxy-1,4-benzoxazin-3-one monooxygenase;
BX5 (gene name);
CYP71C3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (N-hydroxylating)
Reaction(IUBMB)
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [reduced NADPH---hemoprotein reductase] + O2 = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07423]
Reaction(KEGG)
R07423
Substrate
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one [CPD:C15769];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one [CPD:C15770];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
History
EC 1.14.14.110 created 2012 as EC 1.14.13.140, transferred 2018 to EC 1.14.14.110
Pathway
ec00402  Benzoxazinoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13226  2-hydroxy-1,4-benzoxazin-3-one monooxygenase
Genes
ATS: 109753210
ZMA: 103652726
Reference
1  [PMID:16668055]
  Authors
Bailey BA, Larson RL.
  Title
Maize Microsomal Benzoxazinone N-Monooxygenase.
  Journal
Plant Physiol 95:792-796 (1991)
DOI:10.1104/pp.95.3.792
Reference
2  [PMID:10385992]
  Authors
Glawischnig E, Grun S, Frey M, Gierl A
  Title
Cytochrome P450 monooxygenases of DIBOA biosynthesis: specificity and conservation among grasses.
  Journal
Phytochemistry 50:925-30 (1999)
DOI:10.1016/S0031-9422(98)00318-5
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.110
IUBMB Enzyme Nomenclature: 1.14.14.110
ExPASy - ENZYME nomenclature database: 1.14.14.110
BRENDA, the Enzyme Database: 1.14.14.110

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