KEGG   ENZYME: 1.14.14.111
Entry
EC 1.14.14.111              Enzyme                                 
Name
9beta-pimara-7,15-diene oxidase;
CYP99A3;
9beta-pimara-7,15-diene monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
9beta-pimara-7,15-diene,[reduced NADPH---hemoprotein reductase]:oxygen 19-oxidoreductase
Reaction(IUBMB)
9beta-pimara-7,15-diene + 3 O2 + 3 [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R09865];
(1a) 9beta-pimara-7,15-diene + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09862];
(1b) 9beta-pimara-7,15-dien-19-ol + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-al + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R09863];
(1c) 9beta-pimara-7,15-dien-19-al + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09864]
Reaction(KEGG)
R09862 R09863 R09864 R09865;
(other) R09921
Substrate
9beta-pimara-7,15-diene [CPD:C18225];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Product
9beta-pimara-7,15-dien-19-oate [CPD:C20149];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the phytoalexin momilactone. It also acts similarly on 9beta-stemod-13(17)-ene.
History
EC 1.14.14.111 created 2012 as EC 1.14.13.144, transferred 2018 to EC 1.14.14.111
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K16085  9beta-pimara-7,15-diene oxidase
Genes
OSA4335091 4335096
DOSAOs04t0178400-01(Os04g0178400) Os04t0180400-01(Os04g0180400)
OBR102705642 102721444
BDI100836875
ATS109731407 109734305 109743692 109750535 109773965 109773967 109773970 109774538 109776121 109780586 109780589
TDC119271910 119272669 119278132 119281969 119351866 119356226 119358650 119368198
TAES123041873 123041878 123043471 123046476 123048254 123048256 123048257 123048258 123049849 123049853 123054377 123055487 123057860 123063080 123064868 123071979 123074029 123075233 123100267 123126305 123169592 123184294 123184298 123184300 123184301 123184302 123185921 123185949 123190208
SBI8069842 8080269 8081807 8085991
ZMA100273010 103641402 103652787
SITA101767767 101772065 101772739 101776139 101776959
SVS117841995 117842511 117859811 117860031 117860109 117865140
PVIR120651251 120660277 120674828 120690916
PHAI112879668 112896256
 » show all
Reference
1  [PMID:21175892]
  Authors
Wang Q, Hillwig ML, Peters RJ
  Title
CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice.
  Journal
Plant J 65:87-95 (2011)
DOI:10.1111/j.1365-313X.2010.04408.x
  Sequence
[osa:4335091]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.111
IUBMB Enzyme Nomenclature: 1.14.14.111
ExPASy - ENZYME nomenclature database: 1.14.14.111
BRENDA, the Enzyme Database: 1.14.14.111

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