KEGG   ENZYME: 1.14.14.111
Entry
EC 1.14.14.111              Enzyme                                 

Name
9beta-pimara-7,15-diene oxidase;
CYP99A3;
9beta-pimara-7,15-diene monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
9beta-pimara-7,15-diene,[reduced NADPH---hemoprotein reductase]:oxygen 19-oxidoreductase
Reaction(IUBMB)
9beta-pimara-7,15-diene + 3 O2 + 3 [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R09865];
(1a) 9beta-pimara-7,15-diene + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09862];
(1b) 9beta-pimara-7,15-dien-19-ol + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-al + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R09863];
(1c) 9beta-pimara-7,15-dien-19-al + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09864]
Reaction(KEGG)
R09862 R09863 R09864 R09865;
(other) R09921
Substrate
9beta-pimara-7,15-diene [CPD:C18225];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Product
9beta-pimara-7,15-dien-19-oate [CPD:C20149];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the phytoalexin momilactone. It also acts similarly on 9beta-stemod-13(17)-ene.
History
EC 1.14.14.111 created 2012 as EC 1.14.13.144, transferred 2018 to EC 1.14.14.111
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K16085  9beta-pimara-7,15-diene oxidase
Genes
OSA: 4335091 4335096
DOSA: Os04t0178400-01(Os04g0178400) Os04t0180400-01(Os04g0180400)
OBR: 102721444
BDI: 100834069
ATS: 109773970(LOC109773970)
SITA: 101767767 101776959
Reference
1  [PMID:21175892]
  Authors
Wang Q, Hillwig ML, Peters RJ
  Title
CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice.
  Journal
Plant J 65:87-95 (2011)
DOI:10.1111/j.1365-313X.2010.04408.x
  Sequence
[osa:4335091]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.111
IUBMB Enzyme Nomenclature: 1.14.14.111
ExPASy - ENZYME nomenclature database: 1.14.14.111
BRENDA, the Enzyme Database: 1.14.14.111

DBGET integrated database retrieval system