KEGG   ENZYME: 1.14.14.114
Entry
EC 1.14.14.114              Enzyme                                 

Name
amorpha-4,11-diene 12-monooxygenase;
CYP71AV1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
amorpha-4,11-diene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)
Reaction(IUBMB)
amorpha-4,11-diene + 3 O2 + 3 [reduced NADPH---hemoprotein reductase] = artemisinate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R10026];
(1a) amorpha-4,11-diene + O2 + [reduced NADPH---hemoprotein reductase] = artemisinic alcohol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10023];
(1b) artemisinic alcohol + O2 + [reduced NADPH---hemoprotein reductase] = artemisinic aldehyde + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R10024];
(1c) artemisinic aldehyde + O2 + [reduced NADPH---hemoprotein reductase] = artemisinate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10025]
Reaction(KEGG)
Substrate
amorpha-4,11-diene [CPD:C16028];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
artemisinic alcohol [CPD:C20307];
artemisinic aldehyde [CPD:C20308]
Product
artemisinate [CPD:C20309];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
artemisinic alcohol [CPD:C20307];
artemisinic aldehyde [CPD:C20308]
Comment
A cytochrome P-450 (heme-thiolate) protein. Cloned from the plant Artemisia annua (sweet wormwood). Part of the biosynthetic pathway of artemisinin.
History
EC 1.14.14.114 created 2012 as EC 1.14.13.158, transferred 2018 to EC 1.14.14.114
Orthology
K21716  amorpha-4,11-diene 12-monooxygenase
Reference
1  [PMID:16458889]
  Authors
Teoh KH, Polichuk DR, Reed DW, Nowak G, Covello PS
  Title
Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin.
  Journal
FEBS Lett 580:1411-6 (2006)
DOI:10.1016/j.febslet.2006.01.065
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.114
IUBMB Enzyme Nomenclature: 1.14.14.114
ExPASy - ENZYME nomenclature database: 1.14.14.114
BRENDA, the Enzyme Database: 1.14.14.114

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