KEGG   ENZYME: 1.14.14.115
Entry
EC 1.14.14.115              Enzyme                                 
Name
11-oxo-beta-amyrin 30-oxidase;
CYP72A;
CYP72A154;
11-oxo-beta-amyrin 30-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
11-oxo-beta-amyrin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (30-hydroxylating)
Reaction(IUBMB)
11-oxo-beta-amyrin + 3 O2 + 3 [reduced NADPH---hemoprotein reductase] = glycyrrhetinate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R10316];
(1a) 11-oxo-beta-amyrin + O2 + [reduced NADPH---hemoprotein reductase] = 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10313];
(1b) 30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH---hemoprotein reductase] = glycyrrhetaldehyde + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R10314];
(1c) glycyrrhetaldehyde + O2 + [reduced NADPH---hemoprotein reductase] = glycyrrhetinate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10315]
Reaction(KEGG)
Substrate
11-oxo-beta-amyrin [CPD:C19943];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
30-hydroxy-11-oxo-beta-amyrin [CPD:C20510];
glycyrrhetaldehyde [CPD:C20511]
Product
glycyrrhetinate [CPD:C02283];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
30-hydroxy-11-oxo-beta-amyrin [CPD:C20510];
glycyrrhetaldehyde [CPD:C20511]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from the plant Glycyrrhiza uralensis (licorice) is involved in the biosynthesis of the triterpenoid saponin glycyrrhizin. The enzyme from the plant Medicago truncatula can also hydroxylate beta-amyrin.
History
EC 1.14.14.115 created 2013 as EC 1.14.13.173, transferred 2018 to EC 1.14.14.115
Orthology
K17873  11-oxo-beta-amyrin 30-oxidase
Genes
GMX100781923 100790445 102667924
GSJ114376255 114386791 114416844
PVUPHAVU_005G0634001 PHAVU_011G161700g
VRA106762812 106780026 106780477
VAR108334287 108338792 108338865
VUN114168348 114169728 114169729 114185885
CCAJ109795352 109798847 109798864 109813753
APRC113853697 113864694 113864769
MTR11410378 11411801 11415378 25487125
CAM101495615 101500151 101506810
LJALj0g3v0329019.1(Lj0g3v0329019.1) Lj6g3v0920050.3(Lj6g3v0920050.3)
ADU107459746 107459880 107476421 107485529
AIP107613581 107618986 107629316
AHF112703474 112703487 112736390 112772325 112776928 112784743 112797321
LANG109354704
 » show all
Reference
1  [PMID:22128119]
  Authors
Seki H, Sawai S, Ohyama K, Mizutani M, Ohnishi T, Sudo H, Fukushima EO, Akashi T, Aoki T, Saito K, Muranaka T
  Title
Triterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin.
  Journal
Plant Cell 23:4112-23 (2011)
DOI:10.1105/tpc.110.082685
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.115
IUBMB Enzyme Nomenclature: 1.14.14.115
ExPASy - ENZYME nomenclature database: 1.14.14.115
BRENDA, the Enzyme Database: 1.14.14.115

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