KEGG   ENZYME: 1.14.14.115
Entry
EC 1.14.14.115              Enzyme                                 
Name
11-oxo-beta-amyrin 30-oxidase;
CYP72A;
CYP72A154;
11-oxo-beta-amyrin 30-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
11-oxo-beta-amyrin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (30-hydroxylating)
Reaction(IUBMB)
11-oxo-beta-amyrin + 3 O2 + 3 [reduced NADPH---hemoprotein reductase] = glycyrrhetinate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R10316];
(1a) 11-oxo-beta-amyrin + O2 + [reduced NADPH---hemoprotein reductase] = 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10313];
(1b) 30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH---hemoprotein reductase] = glycyrrhetaldehyde + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R10314];
(1c) glycyrrhetaldehyde + O2 + [reduced NADPH---hemoprotein reductase] = glycyrrhetinate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10315]
Reaction(KEGG)
R10313 R10314 R10315 R10316
Substrate
11-oxo-beta-amyrin [CPD:C19943];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
30-hydroxy-11-oxo-beta-amyrin [CPD:C20510];
glycyrrhetaldehyde [CPD:C20511]
Product
glycyrrhetinate [CPD:C02283];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
30-hydroxy-11-oxo-beta-amyrin [CPD:C20510];
glycyrrhetaldehyde [CPD:C20511]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from the plant Glycyrrhiza uralensis (licorice) is involved in the biosynthesis of the triterpenoid saponin glycyrrhizin. The enzyme from the plant Medicago truncatula can also hydroxylate beta-amyrin.
History
EC 1.14.14.115 created 2013 as EC 1.14.13.173, transferred 2018 to EC 1.14.14.115
Orthology
K17873  11-oxo-beta-amyrin 30-oxidase
Genes
GMX: 100781923 100790445 102667924
GSJ: 114376255 114386791 114416844
PVU: PHAVU_005G0634001 PHAVU_011G161700g
VRA: 106762812 106780026 106780477
VAR: 108334287 108338792 108338865
VUN: 114168348 114169728 114169729 114185885
CCAJ: 109795352 109798847 109798864 109813753
APRC: 113853697 113864694 113864769
MTR: 11410378 11411801 11415378 25487125
TPRA: 123894540 123894972 123894993
CAM: 101495615 101500151 101506810
PSAT: 127075638 127075642
LJA: Lj0g3v0329019.1(Lj0g3v0329019.1) Lj6g3v0920050.3(Lj6g3v0920050.3)
ADU: 107459746 107459880 107476421 107485529
AIP: 107613581 107618986 107629316
AHF: 112703474 112703487 112736390 112772325 112776928 112784743 112797321
LANG: 109354704
 » show all
Reference
1  [PMID:22128119]
  Authors
Seki H, Sawai S, Ohyama K, Mizutani M, Ohnishi T, Sudo H, Fukushima EO, Akashi T, Aoki T, Saito K, Muranaka T
  Title
Triterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin.
  Journal
Plant Cell 23:4112-23 (2011)
DOI:10.1105/tpc.110.082685
  Sequence
[ag:BAL45207]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.115
IUBMB Enzyme Nomenclature: 1.14.14.115
ExPASy - ENZYME nomenclature database: 1.14.14.115
BRENDA, the Enzyme Database: 1.14.14.115

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Chemical substance (8)   
   KEGG COMPOUND (8)   
Chemical reaction (8)   
   KEGG REACTION (4)   
   KEGG RCLASS (4)   
Gene (56)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (55)   
Protein sequence (90)   
   UniProt (86)   
   SWISS-PROT (2)   
   RefSeq(pep) (2)   
DNA sequence (3)   
   RefSeq(nuc) (2)   
   GenBank (1)   
Protein domain (4)   
   InterPro (4)   
All databases (169)   

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