KEGG   ENZYME: 1.14.14.116Help
Entry
EC 1.14.14.116              Enzyme                                 

Name
averantin hydroxylase;
AVN hydroxylase;
avnA (gene name);
CYP60A1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(1'S)-averantin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5'-hydroxylating)
Reaction(IUBMB)
(1) (1'S)-averantin + [reduced NADPH---hemoprotein reductase] + O2 = (1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10317];
(2) (1'S)-averantin + [reduced NADPH---hemoprotein reductase] + O2 = (1'S,5'R)-5'-hydroxyaverantin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10318]
Reaction(KEGG)
Substrate
(1'S)-averantin [CPD:C20499];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(1'S,5'S)-5'-hydroxyaverantin [CPD:C20500];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
(1'S,5'R)-5'-hydroxyaverantin [CPD:C20501]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the saprophytic mold Aspergillus parasiticus. Involved in aflatoxin biosynthesis. Does not react with (1'R)-averantin.
History
EC 1.14.14.116 created 2013 as EC 1.14.13.174, transferred 2018 to EC 1.14.14.116
Pathway
ec00254  Aflatoxin biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K17645  averantin hydroxylase
Genes
PAN: PODANSg8563
ANI: AN7818.2
AOR: AO090026000021
AFV: AFLA_139260
Taxonomy
Reference
1  [PMID:8368836]
  Authors
Yabe K, Matsuyama Y, Ando Y, Nakajima H, Hamasaki T
  Title
Stereochemistry during aflatoxin biosynthesis: conversion of norsolorinic acid to averufin.
  Journal
Appl Environ Microbiol 59:2486-92 (1993)
Reference
2  [PMID:9097431]
  Authors
Yu J, Chang PK, Cary JW, Bhatnagar D, Cleveland TE
  Title
avnA, a gene encoding a cytochrome P-450 monooxygenase, is involved in the conversion of averantin to averufin in aflatoxin biosynthesis in Aspergillus parasiticus.
  Journal
Appl Environ Microbiol 63:1349-56 (1997)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.116
IUBMB Enzyme Nomenclature: 1.14.14.116
ExPASy - ENZYME nomenclature database: 1.14.14.116
BRENDA, the Enzyme Database: 1.14.14.116

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