aflatoxin B synthase;
ordA (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
8-O-methylsterigmatocystin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (aflatoxin-B-forming)
(1) 8-O-methylsterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B1 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN:
(2) 8-O-methyldihydrosterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B2 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN:
[reduced NADPH---hemoprotein reductase] [CPD:
A cytochrome P-450 (heme-thiolate) protein. Isolated from the mold Aspergillus parasiticus.
EC 184.108.40.206 created 2013 as EC 220.127.116.11, transferred 2018 to EC 18.104.22.168
ec01110 Biosynthesis of secondary metabolites
Bhatnagar D, Cleveland TE, Kingston DG
Enzymological evidence for separate pathways for aflatoxin B1 and B2 biosynthesis.
Yu J, Chang PK, Ehrlich KC, Cary JW, Montalbano B, Dyer JM, Bhatnagar D, Cleveland TE
Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2.
Udwary DW, Casillas LK, Townsend CA
Synthesis of 11-hydroxyl O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis.