Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

8-O-methylsterigmatocystin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (aflatoxin-B forming)

(1) 8-O-methylsterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B1 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN:R10319];
(2) 8-O-methyldihydrosterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B2 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN:R10320]

R10319 R10320
Reaction

8-O-methylsterigmatocystin [CPD:C03686];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
8-O-methyldihydrosterigmatocystin [CPD:C03944]

A cytochrome P-450 (heme-thiolate) protein. Isolated from the mold Aspergillus parasiticus.

EC 1.14.14.117 created 2013 as EC 1.14.13.175, transferred 2018 to EC 1.14.14.117

1  [PMID:1902378]

Bhatnagar D, Cleveland TE, Kingston DG

Enzymological evidence for separate pathways for aflatoxin B1 and B2 biosynthesis.

Biochemistry 30:4343-50 (1991)
DOI:10.1021/bi00231a033

2  [PMID:9835571]

Yu J, Chang PK, Ehrlich KC, Cary JW, Montalbano B, Dyer JM, Bhatnagar D, Cleveland TE

Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2.

Appl Environ Microbiol 64:4834-41 (1998)
DOI:10.1128/AEM.64.12.4834-4841.1998

[ag:AAS66031]

3  [PMID:11996570]

Udwary DW, Casillas LK, Townsend CA

Synthesis of 11-hydroxyl O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis.

J Am Chem Soc 124:5294-303 (2002)
DOI:10.1021/ja012185v

[ag:AAS66031]