Entry |
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Name |
aflatoxin B synthase;
ordA (gene name)
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
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Sysname |
8-O-methylsterigmatocystin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (aflatoxin-B forming)
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Reaction(IUBMB) |
(1) 8-O-methylsterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B1 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN: R10319];
(2) 8-O-methyldihydrosterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B2 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN: R10320]
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Reaction(KEGG) |
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Substrate |
8-O-methylsterigmatocystin [CPD: C03686];
[reduced NADPH---hemoprotein reductase] [CPD: C03024];
O2 [CPD: C00007];
8-O-methyldihydrosterigmatocystin [CPD: C03944]
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Product |
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Comment |
A cytochrome P-450 (heme-thiolate) protein. Isolated from the mold Aspergillus parasiticus.
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History |
EC 1.14.14.117 created 2013 as EC 1.14.13.175, transferred 2018 to EC 1.14.14.117
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Pathway |
ec01110 | Biosynthesis of secondary metabolites |
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Orthology |
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Genes |
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Reference |
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Authors |
Bhatnagar D, Cleveland TE, Kingston DG |
Title |
Enzymological evidence for separate pathways for aflatoxin B1 and B2 biosynthesis. |
Journal |
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Reference |
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Authors |
Yu J, Chang PK, Ehrlich KC, Cary JW, Montalbano B, Dyer JM, Bhatnagar D, Cleveland TE |
Title |
Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2. |
Journal |
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Sequence |
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Reference |
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Authors |
Udwary DW, Casillas LK, Townsend CA |
Title |
Synthesis of 11-hydroxyl O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis. |
Journal |
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Sequence |
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Other DBs |
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