Entry
Name
aflatoxin B synthase;
ordA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
8-O-methylsterigmatocystin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (aflatoxin-B-forming)
Reaction(IUBMB)
(1) 8-O-methylsterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B1 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN:
R10319 ];
(2) 8-O-methyldihydrosterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B2 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN:
R10320 ]
Reaction(KEGG)
Substrate
8-O-methylsterigmatocystin [CPD:
C03686 ];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024 ];
O2 [CPD:
C00007 ];
8-O-methyldihydrosterigmatocystin [CPD:
C03944 ]
Product
Comment
A cytochrome P-450 (heme-thiolate) protein. Isolated from the mold Aspergillus parasiticus.
History
EC 1.14.14.117 created 2013 as EC 1.14.13.175, transferred 2018 to EC 1.14.14.117
Pathway
ec01110 Biosynthesis of secondary metabolites
Orthology
Genes
Taxonomy
Reference
Authors
Bhatnagar D, Cleveland TE, Kingston DG
Title
Enzymological evidence for separate pathways for aflatoxin B1 and B2 biosynthesis.
Journal
Reference
Authors
Yu J, Chang PK, Ehrlich KC, Cary JW, Montalbano B, Dyer JM, Bhatnagar D, Cleveland TE
Title
Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2.
Journal
Sequence
Reference
Authors
Udwary DW, Casillas LK, Townsend CA
Title
Synthesis of 11-hydroxyl O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis.
Journal
Sequence
Other DBs