KEGG   ENZYME: 1.14.14.12Help
Entry
EC 1.14.14.12               Enzyme                                 

Name
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase;
HsaA
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione,FMNH2:oxygen oxidoreductase
Reaction(IUBMB)
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH2 + O2 = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H2O [RN:R09819]
Reaction(KEGG)
Substrate
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C19944];
FMNH2 [CPD:C01847];
O2 [CPD:C00007]
Product
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C04793];
FMN [CPD:C00061];
H2O [CPD:C00001]
Comment
This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone. It can use either FADH or FMNH2 as flavin cofactor. The enzyme forms a two-component system with a reductase (HsaB) that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit.
History
EC 1.14.14.12 created 2011
Pathway
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K16047  3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
Genes
RGL: CS053_01745
VAF: D1115_22760
PRE: PCA10_43690
PCQ: PcP3B5_25640(hsaA_1) PcP3B5_37050(hsaA_3) PcP3B5_37450(hsaA_4)
PPUT: L483_14090
PPUN: PP4_27210
PFE: PSF113_3461 PSF113_3462
PVR: PverR02_11725
PBA: PSEBR_a976
PPUU: PputUW4_01682
PKC: PKB_4323
PSET: THL1_4411
SPL: Spea_2129
SHL: Shal_2101
PHA: PSHAa0877
PTN: PTRA_a1004(hsaA)
PNG: PNIG_a1056(hsaA)
RSY: RSUY_11610(hsaA)
REH: H16_B0638(h16_B0638)
CNC: CNE_2c05830(hsaA)
CGD: CR3_0799(hsaA)
BCJ: BCAM1647
BCEN: DM39_6202
BCEO: I35_5507
BAM: Bamb_5026
BCT: GEM_4008
BCED: DM42_6311
BCON: NL30_03950
BTEI: WS51_05860
BPSL: WS57_05845
BMEC: WJ16_26975
BGO: BM43_6910
BFN: OI25_4616
BAV: BAV0595
CTES: O987_07755
DAR: Daro_0902
BJA: bll6096
BRS: S23_21790
BRAD: BF49_5537
NWI: Nwi_0937
NHA: Nham_3406
XAU: Xaut_0933
SNO: Snov_0210
CSE: Cseg_1728
SPHM: G432_01990
STAX: MC45_16520
SPHI: TS85_13285
SSAN: NX02_12720
SSY: SLG_18380
SPHB: EP837_03391(hsaA)
SMIC: SmB9_33370
MTU: Rv3570c(hsaA)
MTC: MT3675
MRA: MRA_3609
MTUR: CFBS_3787
MTD: UDA_3570c
MTUC: J113_25000
MTUE: J114_19095
MTUH: I917_25085
MTUL: TBHG_03511
MTUT: HKBT1_3772
MTUU: HKBT2_3781
MBO: BQ2027_MB3601C(hsaa)
MBB: BCG_3635c
MBT: JTY_3636
MBX: BCGT_3437
MAF: MAF_35830
MMIC: RN08_3947
MCQ: BN44_110065(hsaA)
MCV: BN43_90071(hsaA)
MCX: BN42_90068(hsaA)
MCZ: BN45_100084(hsaA)
MLP: MLM_0735
MHAD: B586_04445
MSHG: MSG_00802(hsaA_1) MSG_02472 MSG_04563(hsaA_2)
MPHL: MPHLCCUG_00491(hsaA_1) MPHLCCUG_01764(hsaA_2) MPHLCCUG_02628(hsaA_4) MPHLCCUG_03656(hsaA_5) MPHLCCUG_03657(hsaA_6)
MTHN: 4412656_00960(hsaA_1) 4412656_01361(hsaA_3) 4412656_04094(hsaA_5)
MHAS: MHAS_00635(hsaA_1) MHAS_02456(hsaA_2) MHAS_03779(hsaA_3)
MAB: MAB_0584
MSAL: DSM43276_00265(hsaA_1) DSM43276_00502(hsaA_2)
MTER: 4434518_02237(hsaA_2) 4434518_03729(hsaA_3) 4434518_04155(hsaA_4)
ASD: AS9A_0924
NFR: ERS450000_00305(hsaA_1) ERS450000_00680(hsaA_2) ERS450000_00694(hsaA_3) ERS450000_01850(hsaA_6) ERS450000_03762(hsaA_8)
RHA: RHA1_ro04539(hsaA)
RHB: NY08_3127
RHU: A3Q40_00865(hsaA)
RRT: 4535765_00582(hsaA_1) 4535765_00630(hsaA_2) 4535765_00632(hsaA_3) 4535765_01483(hsaA_4) 4535765_04054(hsaA_6) 4535765_04572(hsaA_7) 4535765_04586(hsaA_8)
GBR: Gbro_0920
GOR: KTR9_0820(hsaA)
GRU: GCWB2_04175(hsaA1) GCWB2_08185(hsaA2)
GOM: D7316_02455(hsaA_1) D7316_02566(hsaA_2)
TPR: Tpau_3845
SRT: Srot_0299
SHY: SHJG_0396
SALS: SLNWT_2215
SFI: SFUL_4664
SALU: DC74_7445
SALL: SAZ_38430
STRE: GZL_08548
STRM: M444_37895
SMAL: SMALA_8231
SALJ: SMD11_0294
SLX: SLAV_27430(hsaA1)
SGE: DWG14_00038(hsaA_1) DWG14_00204(hsaA_2) DWG14_08423(hsaA_3)
NDK: I601_0579(hsaA_1) I601_3470(hsaA_2)
SRO: Sros_6977
AMD: AMED_2522
AJA: AJAP_26405(hsaA)
AMYY: YIM_14450(hsaA1) YIM_23025(hsaA2) YIM_36670(hsaA6) YIM_40880(hsaA8)
PAUT: Pdca_45790
KAL: KALB_5124
AHG: AHOG_16455(hsaA1)
SAQ: Sare_2879
TBI: Tbis_1633
CWO: Cwoe_2133
TRO: trd_A0928
 » show all
Taxonomy
Reference
1  [PMID:20448045]
  Authors
Dresen C, Lin LY, D'Angelo I, Tocheva EI, Strynadka N, Eltis LD
  Title
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism.
  Journal
J Biol Chem 285:22264-75 (2010)
DOI:10.1074/jbc.M109.099028
  Sequence
[mtu:Rv3570c]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.12
IUBMB Enzyme Nomenclature: 1.14.14.12
ExPASy - ENZYME nomenclature database: 1.14.14.12
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.14.12
BRENDA, the Enzyme Database: 1.14.14.12

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