KEGG   ENZYME: 1.14.14.120
Entry
EC 1.14.14.120              Enzyme                                 
Name
dammarenediol 12-hydroxylase;
protopanaxadiol synthase;
CYP716A47
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
dammarenediol-II,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12beta-hydroxylating)
Reaction(IUBMB)
dammarenediol-II + [reduced NADPH---hemoprotein reductase] + O2 = protopanaxadiol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10540]
Reaction(KEGG)
R10540
Substrate
dammarenediol-II [CPD:C19829];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
protopanaxadiol [CPD:C20715];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from ginseng (Panax ginseng). Involved in the biosynthetic pathway of ginsenosides.
History
EC 1.14.14.120 created 2013 as EC 1.14.13.183, transferred 2018 to EC 1.14.14.120
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
Orthology
K20545  dammarenediol 12-hydroxylase
Reference
1  [PMID:22039120]
  Authors
Han JY, Kim HJ, Kwon YS, Choi YE
  Title
The Cyt P450 enzyme CYP716A47 catalyzes the formation of protopanaxadiol from dammarenediol-II during ginsenoside biosynthesis in Panax ginseng.
  Journal
Plant Cell Physiol 52:2062-73 (2011)
DOI:10.1093/pcp/pcr150
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.120
IUBMB Enzyme Nomenclature: 1.14.14.120
ExPASy - ENZYME nomenclature database: 1.14.14.120
BRENDA, the Enzyme Database: 1.14.14.120

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