KEGG   ENZYME: 1.14.14.125
Entry
EC 1.14.14.125              Enzyme                                 

Name
monacolin L hydroxylase;
LovA (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
monacolin L acid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (8-hydroxylating)
Reaction(IUBMB)
monacolin L acid + O2 + [reduced NADPH---hemoprotein reductase] = monacolin J acid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10794]
Reaction(KEGG)
R10794
Substrate
monacolin L acid [CPD:C20853];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024]
Product
monacolin J acid [CPD:C20854];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from fungi also catalyses the reaction of EC 1.14.14.124, dihydromonacolin L hydroxylase.
History
EC 1.14.14.125 created 2014 as EC 1.14.13.198, transferred 2018 to EC 1.14.14.125
Pathway
ec00999  Biosynthesis of various secondary metabolites - part 1
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20980  dihydromonacolin L/monacolin L hydroxylase
Reference
1  [PMID:21495633]
  Authors
Barriuso J, Nguyen DT, Li JW, Roberts JN, MacNevin G, Chaytor JL, Marcus SL, Vederas JC, Ro DK
  Title
Double oxidation of the cyclic nonaketide dihydromonacolin L to monacolin J by a  single cytochrome P450 monooxygenase, LovA.
  Journal
J Am Chem Soc 133:8078-81 (2011)
DOI:10.1021/ja201138v
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.125
IUBMB Enzyme Nomenclature: 1.14.14.125
ExPASy - ENZYME nomenclature database: 1.14.14.125
BRENDA, the Enzyme Database: 1.14.14.125

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