KEGG   ENZYME: 1.14.14.129
Entry
EC 1.14.14.129              Enzyme                                 

Name
long-chain acyl-CoA omega-monooxygenase;
long-chain acyl-CoA omega-hydroxylase;
CYP86A22 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
long-chain acyl-CoA,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (omega-hydroxylating)
Reaction(IUBMB)
(1) oleoyl-CoA + [reduced NADPH---hemoprotein reductase] + O2 = 18-hydroxyoleoyl-CoA + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10999];
(2) linoleoyl-CoA + [reduced NADPH---hemoprotein reductase] + O2 = 18-hydroxylinoleoyl-CoA + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11000]
Reaction(KEGG)
R10999 R11000
Substrate
oleoyl-CoA [CPD:C00510];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
linoleoyl-CoA [CPD:C02050]
Product
18-hydroxyoleoyl-CoA [CPD:C21005];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
18-hydroxylinoleoyl-CoA [CPD:C21006]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzymes from solanaceous plants are involved in the biosynthesis of stigmatic estolide, a lipid-based polyester that forms a major component of the exudate.
History
EC 1.14.14.129 created 2015 as EC 1.14.13.204, transferred 2018 to EC 1.14.14.129
Orthology
K20544  long-chain acyl-CoA omega-monooxygenase
Genes
SLY: 101254719
SPEN: 107028839
SOT: 102594638
CANN: 107865299
NTA: 107771909 107811390
NSY: 104243883
NTO: 104103878
NAU: 109220597
INI: 109147653
SIND: 105157979
 » show all
Reference
1  [PMID:19940120]
  Authors
Han J, Clement JM, Li J, King A, Ng S, Jaworski JG
  Title
The cytochrome P450 CYP86A22 is a fatty acyl-CoA omega-hydroxylase essential for Estolide synthesis in the stigma of Petunia hybrida.
  Journal
J Biol Chem 285:3986-96 (2010)
DOI:10.1074/jbc.M109.050765
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.129
IUBMB Enzyme Nomenclature: 1.14.14.129
ExPASy - ENZYME nomenclature database: 1.14.14.129
BRENDA, the Enzyme Database: 1.14.14.129

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