KEGG   ENZYME: 1.14.14.132
Entry
EC 1.14.14.132              Enzyme                                 

Name
(-)-4'-demethyl-deoxypodophyllotoxin 4-hydroxylase;
CYP82D61 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(-)-deoxypodophyllotoxin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
(-)-4'-demethyldeoxypodophyllotoxin + [reduced NADPH---hemoprotein reductase] + O2 = (-)-4'-demethylepipodophyllotoxin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11212]
Reaction(KEGG)
R11212
Substrate
(-)-4'-demethyldeoxypodophyllotoxin [CPD:C10552];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(-)-4'-demethylepipodophyllotoxin [CPD:C21185];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein characterized from the plant Sinopodophyllum hexandrum. The enzyme produces the direct precursor to etoposide, a potent anticancer drug. It can also act on (-)-deoxypodophyllotoxin with lower efficiency.
History
EC 1.14.14.132 created 2016 as EC 1.14.13.214, transferred 2018 to EC 1.14.14.132
Orthology
K21206  (-)-4'-demethyl-deoxypodophyllotoxin 4-hydroxylase
Reference
1  [PMID:26359402]
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.132
IUBMB Enzyme Nomenclature: 1.14.14.132
ExPASy - ENZYME nomenclature database: 1.14.14.132
BRENDA, the Enzyme Database: 1.14.14.132

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