KEGG   ENZYME: 1.14.14.134Help
Entry
EC 1.14.14.134              Enzyme                                 

Name
beta-amyrin 24-hydroxylase;
sophoradiol 24-hydroxylase;
CYP93E1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
beta-amyrin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (24-hydroxylating)
Reaction(IUBMB)
(1) beta-amyrin + [reduced NADPH---hemoprotein reductase] + O2 = 24-hydroxy-beta-amyrin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09604];
(2) sophoradiol + [reduced NADPH---hemoprotein reductase] + O2 = 24-hydroxysophoradiol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09605]
Reaction(KEGG)
Substrate
beta-amyrin [CPD:C08616];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
sophoradiol [CPD:C19733]
Product
24-hydroxy-beta-amyrin [CPD:C19732];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
24-hydroxysophoradiol [CPD:C08980]
Comment
A cytochrome P-450 (heme-thiolate) protein. Found in plants and participates in the biosynthesis of soybean saponins.
History
EC 1.14.14.134 created 2011 as EC 1.14.99.43, transferred 2018 to EC 1.14.14.134
Pathway
ec00909  Sesquiterpenoid and triterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K15814  beta-amyrin 24-hydroxylase
Genes
GMX: 100037459(CYP93E1)
PVU: PHAVU_008G128700g
VRA: 106758614
VAR: 108327766
MTR: MTR_7g056103
CAM: 101495076
LJA: Lj1g3v3555800.1(Lj1g3v3555800.1)
ADU: 107484048
AIP: 107639472
LANG: 109352528
Taxonomy
Reference
1  [PMID:16478469]
  Authors
Shibuya M, Hoshino M, Katsube Y, Hayashi H, Kushiro T, Ebizuka Y
  Title
Identification of beta-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay.
  Journal
FEBS J 273:948-59 (2006)
DOI:10.1111/j.1742-4658.2006.05120.x
  Sequence
[gmx:100037459]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.134
IUBMB Enzyme Nomenclature: 1.14.14.134
ExPASy - ENZYME nomenclature database: 1.14.14.134
BRENDA, the Enzyme Database: 1.14.14.134

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