KEGG ENZYME: 1.14.14.135

ENZYME: 1.14.14.135

Entry

EC 1.14.14.135              Enzyme

Name

glyceollin synthase;
dimethylallyl-3,6a,9-trihydroxypterocarpan cyclase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

(6aS,11aS)-3,6a,9-trihydroxy-2-prenylpterocarpan,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (cyclizing)

Reaction(IUBMB)

(1) (6aS,11aS)-3,6a,9-trihydroxy-2-prenylpterocarpan + [reduced NADPH---hemoprotein reductase] + O2 = glyceollin II + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R07195];
(2) (6aS,11aS)-3,6a,9-trihydroxy-2-prenylpterocarpan + [reduced NADPH---hemoprotein reductase] + O2 = glyceollin III + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R07196];
(3) (6aS,11aS)-3,6a,9-trihydroxy-4-prenylpterocarpan + [reduced NADPH---hemoprotein reductase] + O2 = glyceollin I + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R07197]

Reaction(KEGG)

R07195 R07196 R07197;
(other) R07356 R07357

Substrate

(6aS,11aS)-3,6a,9-trihydroxy-2-prenylpterocarpan [CPD:C15509];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
(6aS,11aS)-3,6a,9-trihydroxy-4-prenylpterocarpan [CPD:C15510]

Product

glyceollin II;
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
glyceollin III [CPD:C15511];
glyceollin I

Comment

A cytochrome P-450 (heme-thiolate) protein purified from soybean.

History

EC 1.14.14.135 created 2004 as EC 1.14.13.85, transferred 2018 to EC 1.14.14.135

Pathway

ec00943

Isoflavonoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Reference