KEGG   ENZYME: 1.14.14.135Help
Entry
EC 1.14.14.135              Enzyme                                 

Name
glyceollin synthase;
dimethylallyl-3,6a,9-trihydroxypterocarpan cyclase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (cyclizing)
Reaction(IUBMB)
(1) 2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [reduced NADPH---hemoprotein reductase] + O2 = glyceollin II + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R07195];
(2) 2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [reduced NADPH---hemoprotein reductase] + O2 = glyceollin III + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R07196];
(3) 4-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [reduced NADPH---hemoprotein reductase] + O2 = glyceollin I + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R07197]
Reaction(KEGG)
Substrate
2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan [CPD:C15509];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
4-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan [CPD:C15510]
Product
glyceollin II;
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
glyceollin III [CPD:C15511];
glyceollin I
Comment
A cytochrome P-450 (heme-thiolate) protein purified from soybean.
History
EC 1.14.14.135 created 2004 as EC 1.14.13.85, transferred 2018 to EC 1.14.14.135
Pathway
ec00943  Isoflavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Reference
1  [PMID:3369863]
  Authors
Welle R, Grisebach H.
  Title
Induction of phytoalexin synthesis in soybean: enzymatic cyclization of prenylated pterocarpans to glyceollin isomers.
  Journal
Arch Biochem Biophys 263:191-8 (1988)
DOI:10.1016/0003-9861(88)90627-3
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.135
IUBMB Enzyme Nomenclature: 1.14.14.135
ExPASy - ENZYME nomenclature database: 1.14.14.135
BRENDA, the Enzyme Database: 1.14.14.135

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