KEGG   ENZYME: 1.14.14.147
Entry
EC 1.14.14.147              Enzyme                                 
Name
22alpha-hydroxysteroid 23-monooxygenase;
cytochrome P450 90C1;
CYP90D1;
CYP90C1;
3-epi-6-deoxocathasterone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (C-23-hydroxylating);
3-epi-6-deoxocathasterone 23-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
22alpha-hydroxysteroid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (C-23-hydroxylating)
Reaction(IUBMB)
(1) 3-epi-6-deoxocathasterone + [reduced NADPH---hemoprotein reductase] + O2 = 6-deoxotyphasterol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07792];
(2) (22S,24R)-22-hydroxy-5alpha-ergostan-3-one + [reduced NADPH---hemoprotein reductase] + O2 = 3-dehydro-6-deoxoteasterone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07791]
Reaction(KEGG)
R07791 R07792;
(other) R07786 R07787
Substrate
3-epi-6-deoxocathasterone [CPD:C16251];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
(22S,24R)-22-hydroxy-5alpha-ergostan-3-one [CPD:C15797]
Product
6-deoxotyphasterol [CPD:C15801];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
3-dehydro-6-deoxoteasterone [CPD:C15800]
Comment
A cytochrome P-450 (heme-thiolate) protein involved in brassinosteroid biosynthesis in plants. The enzyme has a relaxed substrate specificity, and C-23 hydroxylation can occur at different stages in the pathway. In Arabidopsis thaliana two isozymes, encoded by the CYP90C1 and CYP90D1 genes, have redundant activities.
History
EC 1.14.14.147 created 2010 as EC 1.14.13.112, transferred 2018 to EC 1.14.14.147, modified 2022
Pathway
ec00905  Brassinosteroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12637  3-epi-6-deoxocathasterone 23-monooxygenase
K12638  3-epi-6-deoxocathasterone 23-monooxygenase
Genes
ATHAT3G13730(CYP90D1) AT4G36380(ROT3)
ALY9298835 9303091 9321051
CRB17877976 17893533
CSAT104716496(ROT3) 104721136 104733119 104745632 104765165
EUSEUTSA_v10020591mg EUTSA_v10024901mg
BRP103842602 103862467 103868633 103870101
BNA106350972 106351089 106369862 106409581 106426522 106440615 106451959 106452300
BOE106302041 106307279 106327160 106344076
RSZ108805921 108839589 108849049 108857636
THJ104807476 104810577
CPAP110818395 110822476
CIT102610008 102623590
CICCICLE_v10025448mg CICLE_v10025479mg
PVY116106176 116118949
MINC123198912 123205796 123219507 123219819
TCC18596114 18610843
GRA105769910 105787502 105803759
GHI107906110 107913036 107936555 107943285 121209822
GAB108451933 108458395 108472765
DZI111274623 111280433 111293281 111301082 111316248
EGR104422732 104449963
GMX100782687 100797868 100799030 100812489
GSJ114375691 114381782 114417944 114420172 114422309
PVUPHAVU_002G167400g PHAVU_003G258400g PHAVU_010G139600g
VRA106768728 106773857 106777471
VAR108323332 108335338 108336674
VUN114166334 114174236 114179716
CCAJ109801110 109809062 109814118
APRC113851021 113856000 113858772 113870295
MTR11405666 11406632 25500440
CAM101488381 101505609 101509993
PSAT127074637 127076400 127076401 127120195
LJALj2g3v1728770.1(Lj2g3v1728770.1) Lj2g3v1728770.2(Lj2g3v1728770.2) Lj3g3v2693130.1(Lj3g3v2693130.1) Lj3g3v2693130.2(Lj3g3v2693130.2)
ADU107461434 107464245 107488015
AIP107612705 107618558 107642922
AHF112706501 112710103 112749491 112772650 112777343 112800770
LANG109348710 109358278
FVE101297721 101302700
RCN112187971 112188662
PPER18771246 18784338
PMUM103327717 103340160
PAVI110767804 110773923
PDUL117622280 117636128
MDM103400874 103431557 103453669
PXB103934102 103957186
ZJU107403831 107424559
MNT112094164 21388039
CSV101203911 101212581 101217830
CMO103485087 103490016 103500734
BHJ120073232 120086051 120091319
MCHA111005423 111016959
CMAX111479936 111496682
CMOS111458064 111463512
CPEP111782027 111790258
RCU8266971 8279118
JCU105631477 105639077
HBR110639585 110648648 110668522
MESC110608590 110626427
POP18096744 7465155 7495513
PEU105114341 105118255 105118442 105126648
PALZ118030995 118040884 118063091
JRE108991288 108992064 109007914
CILL122281481 122300963 122317271
QSU111995943 112000874 112003250
QLO115962687 115989007 115994450
TWL120013070 120015527
VVI100259060 100259945
VRI117912322 117921515
SLY101245605 101256170
SPEN107009038 107014078
SOT102582597 102598106
SSTN125849542 125859968
CANN107860019 107861744
NTA107771928 107795325 107795780 107816861
NSY104213362 104213597
NTO104118978 104121493
NAU109210485 109231818
INI109177767 109190236
ITR116000340 116023130
SIND105157742 105165748
OEU111367697
EGT105965330 105975773
SSPL121767418 121768407 121771701 121771730
APAN127244242 127262812
HAN110886538 110894209 110915370
ECAD122579553 122596266
LSV111897310 111910313 122194394
CCAV112513182 112517254
DCR108219828 108220552
CSIN114270454 114279029 114309759
BVG104895094 104900369
SOE110785866 110798894
CQI110682556 110684296 110685861 110719834
NNU104586612 104595594 104610557
MING122074479 122078744 122089610
TSS122653626 122661980 122667105
PSOM113288714 113288788 113347873 113349511
PDA103712873 103718648 103723970
EGU105040406 105042258 105047955 105059996
MUS103969391 103975346 103991264 103995745 103999307
ZOF121976062 122044032
DCT110094797 110107619
PEQ110020222 110022604
AOF109828140 109835455
NCOL116256449 116256903
ATR18429333 18448141
SMOSELMODRAFT_151754(ROT3B-1) SELMODRAFT_157387(CYP90E3) SELMODRAFT_182839(ROT3A-2)
 » show all
Reference
1  [PMID:15703058]
  Authors
Kim GT, Fujioka S, Kozuka T, Tax FE, Takatsuto S, Yoshida S, Tsukaya H.
  Title
CYP90C1 and CYP90D1 are involved in different steps in the brassinosteroid biosynthesis pathway in Arabidopsis thaliana.
  Journal
Plant J 41:710-21 (2005)
DOI:10.1111/j.1365-313X.2004.02330.x
Reference
2  [PMID:17138693]
  Authors
Ohnishi T, Szatmari AM, Watanabe B, Fujita S, Bancos S, Koncz C, Lafos M, Shibata K, Yokota T, Sakata K, Szekeres M, Mizutani M
  Title
C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis.
  Journal
Plant Cell 18:3275-88 (2006)
DOI:10.1105/tpc.106.045443
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.147
IUBMB Enzyme Nomenclature: 1.14.14.147
ExPASy - ENZYME nomenclature database: 1.14.14.147
BRENDA, the Enzyme Database: 1.14.14.147

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