Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

(-)-vetispiradiene,[reduced NADPH---hemoprotein reductase]:oxygen 2alpha-oxidoreductase

(-)-vetispiradiene + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = solavetivone + 2 [oxidized NADPH---hemoprotein reductase] + 3 H2O (overall reaction) [RN:R10073];
(1a) (-)-vetispiradiene + [reduced NADPH---hemoprotein reductase] + O2 = solavetivol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09576];
(1b) solavetivol + [reduced NADPH---hemoprotein reductase] + O2 = solavetivone + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R09577]

R09576 R09577 R10073
Reaction

(-)-vetispiradiene [CPD:C12142];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
solavetivol [CPD:C19711]

A cytochrome P-450 (heme-thiolate) protein. The enzyme from the plant Hyoscymus muticus also hydroxylates valencene at C-2 to give the alpha-hydroxy compound, nootkatol, and this is converted into nootkatone. 5-Epiaristolochene and epieremophilene are hydroxylated at C-2 to give a 2beta-hydroxy derivatives that are not oxidized further.

EC 1.14.14.151 created 2011 as EC 1.14.13.121, transferred 2018 to EC 1.14.14.151

1  [PMID:17715131]

Takahashi S, Yeo YS, Zhao Y, O'Maille PE, Greenhagen BT, Noel JP, Coates RM, Chappell J

Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates.

J Biol Chem 282:31744-54 (2007)
DOI:10.1074/jbc.M703378200

[ag:ABS00393]