KEGG   ENZYME: 1.14.14.152
Entry
EC 1.14.14.152              Enzyme                                 

Name
beta-amyrin 11-oxidase;
CYP88D6
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
beta-amyrin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
beta-amyrin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = 11-oxo-beta-amyrin + 2 [oxidized NADPH---hemoprotein reductase] + 3 H2O (overall reaction) [RN:R09817];
(1a) beta-amyrin + [reduced NADPH---hemoprotein reductase] + O2 = 11alpha-hydroxy-beta-amyrin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09815];
(1b) 11alpha-hydroxy-beta-amyrin + [reduced NADPH---hemoprotein reductase] + O2 = 11-oxo-beta-amyrin + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R09816]
Reaction(KEGG)
Substrate
beta-amyrin [CPD:C08616];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
11alpha-hydroxy-beta-amyrin [CPD:C19942]
Product
11-oxo-beta-amyrin [CPD:C19943];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
11alpha-hydroxy-beta-amyrin [CPD:C19942]
Comment
A cytochrome P-450 (heme-thiolate) protein from the plant Glycyrrhiza uralensis (Chinese licorice) that participates in the glycyrrhizin biosynthesis pathway. The enzyme is also able to oxidize 30-hydroxy-beta-amyrin to 11alpha,30-dihydroxy-beta-amyrin but this is not thought to be part of glycyrrhizin biosynthesis.
History
EC 1.14.14.152 created 2011 as EC 1.14.13.134, transferred 2018 to EC 1.14.14.152
Orthology
K17875  beta-amyrin 11-oxidase
Genes
VRA: 106773832
VAR: 108346707
VUN: 114166655 114166656
CCAJ: 109791034 109814269
MTR: MTR_4g005230 MTR_8g012970 MTR_8g089800
CAM: 101495501 101495829 101496150 101496479
LJA: Lj3g3v0730100.1(Lj3g3v0730100.1) Lj3g3v1983610.1(Lj3g3v1983610.1) Lj3g3v1983620.1(Lj3g3v1983620.1) Lj3g3v1983620.2(Lj3g3v1983620.2) Lj3g3v1993700.1(Lj3g3v1993700.1) Lj3g3v1993710.1(Lj3g3v1993710.1)
LANG: 109347422
Reference
1  [PMID:18779566]
  Authors
Seki H, Ohyama K, Sawai S, Mizutani M, Ohnishi T, Sudo H, Akashi T, Aoki T, Saito K, Muranaka T
  Title
Licorice beta-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin.
  Journal
Proc Natl Acad Sci U S A 105:14204-9 (2008)
DOI:10.1073/pnas.0803876105
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.152
IUBMB Enzyme Nomenclature: 1.14.14.152
ExPASy - ENZYME nomenclature database: 1.14.14.152
BRENDA, the Enzyme Database: 1.14.14.152

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