KEGG   ENZYME: 1.14.14.155Help
Entry
EC 1.14.14.155              Enzyme                                 

Name
3,6-diketocamphane 1,2-monooxygenase;
3,6-diketocamphane lactonizing enzyme;
3,6-DKCMO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(-)-bornane-2,5-dione,FMNH2:oxygen oxidoreductase (1,2-lactonizing)
Reaction(IUBMB)
(-)-bornane-2,5-dione + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O [RN:R12149]
Reaction(KEGG)
Substrate
(-)-bornane-2,5-dione [CPD:C21929];
O2 [CPD:C00007];
FMNH2 [CPD:C01847]
Product
(-)-5-oxo-1,2-campholide [CPD:C21930];
FMN [CPD:C00061];
H2O [CPD:C00001]
Comment
A Baeyer-Villiger monooxygenase isolated from camphor-grown strains of Pseudomonas putida and encoded on the cam plasmid. Involved in the degradation of (-)-camphor. Requires a dedicated NADH---FMN reductase [cf. EC 1.5.1.42, FMN reductase (NADH)] [1,2]. The product spontaneously converts to [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate.
History
EC 1.14.14.155 created 2018
Orthology
K23020  3,6-diketocamphane 1,6-monooxygenase
Genes
PSET: THL1_4129
NAR: Saro_0522
NRE: BES08_04050
SGI: SGRAN_3241
SPHD: HY78_03445
SCH: Sphch_3057
SBD: ATN00_18810
SPHT: K426_23019
RHA: RHA1_ro05412
Taxonomy
Reference
1  [PMID:23524667]
  Authors
Iwaki H, Grosse S, Bergeron H, Leisch H, Morley K, Hasegawa Y, Lau PC
  Title
Camphor pathway redux: functional recombinant expression of 2,5- and 3,6-diketocamphane monooxygenases of Pseudomonas putida ATCC 17453 with their cognate flavin reductase catalyzing Baeyer-Villiger reactions.
  Journal
Appl Environ Microbiol 79:3282-93 (2013)
DOI:10.1128/AEM.03958-12
  Sequence
Reference
2  [PMID:26527149]
  Authors
Isupov MN, Schroder E, Gibson RP, Beecher J, Donadio G, Saneei V, Dcunha SA, McGhie EJ, Sayer C, Davenport CF, Lau PC, Hasegawa Y, Iwaki H, Kadow M, Balke K, Bornscheuer UT, Bourenkov G, Littlechild JA
  Title
The oxygenating constituent of 3,6-diketocamphane monooxygenase from the CAM plasmid of Pseudomonas putida: the first crystal structure of a type II Baeyer-Villiger monooxygenase.
  Journal
Acta Crystallogr D Biol Crystallogr 71:2344-53 (2015)
DOI:10.1107/S1399004715017939
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.155
IUBMB Enzyme Nomenclature: 1.14.14.155
ExPASy - ENZYME nomenclature database: 1.14.14.155
BRENDA, the Enzyme Database: 1.14.14.155

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