KEGG   ENZYME: 1.14.14.158
Entry
EC 1.14.14.158              Enzyme                                 
Name
carotenoid epsilon hydroxylase;
CYP97C1;
LUT1;
CYP97C;
carotene epsilon-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
alpha-carotene,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
(1) alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2 = alpha-cryptoxanthin + [oxidized NADPH-hemoprotein reductase] + H2O [RN:R07850];
(2) zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 = lutein + [oxidized NADPH-hemoprotein reductase] + H2O [RN:R07531]
Reaction(KEGG)
R07531 R07850
Substrate
alpha-carotene [CPD:C05433];
[reduced NADPH-hemoprotein reductase];
O2 [CPD:C00007];
zeinoxanthin [CPD:C08590]
Product
alpha-cryptoxanthin [CPD:C15981];
[oxidized NADPH-hemoprotein reductase];
H2O [CPD:C00001];
lutein [CPD:C08601]
Comment
A cytochrome P-450 (heme-thiolate) protein.
History
EC 1.14.14.158 created 2011 as EC 1.14.99.45, transferred 2018 to EC 1.14.14.158
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09837  carotenoid epsilon hydroxylase
Genes
ATHAT3G53130(LUT1)
ALY9313971
CRB17887227
CSAT104711869 104781166 104791575
EUSEUTSA_v10010267mg EUTSA_v10023482mg
BRP103850280
BNA106352760 106452442
BOE106300296
RSZ108814454
THJ104818583
CPAP110822860
CIT102625870
CICCICLE_v10011420mg
PVY116143852
MINC123228821
TCC18598092
GRA105761792
GHI121209533 121223568
GAB108470606
HSYR120158085
DZI111296450
GMX100797221 100803324
GSJ114372632 114382715
PVUPHAVU_007G2265001
VRA106772401
VAR108326589
VUN114192824
VUM124828972
CCAJ109818061
APRC113872166
MTR11429551
TPRA123907402
CAM101497194 101497524
PSAT127093315
VVO131644652
LJALj5g3v0681210.1(Lj5g3v0681210.1)
ADU107476248
AIP107626444
AHF112728911 112759486
LANG109360988
PCIN129304715
QSAO6P43_006591
FVE101308016
RCN112191404
PPER18771385
PMUM103339783
PAVI110747444
PDUL117635766
MDM103454736
MSYL126599597
PXB103933149 103964559
ZJU107432039
MNT21405423
CSAV115723298
CSV101204961
CMO103500849
BHJ120073806
MCHA111013687
CMAX111471281
CMOS111463211
CPEP111782219
RCU8283943
MEAN126678007
JCU105642313
HBR110662670
MESC110620012
POP18100110
PEU105121750
PALZ118048373
JRE108985270 118347955
CILL122275262
CAVE132191717
QSU111984908
QLO115953099
TWL119985060
VVI100243621
VRI117920315 117920676
SLY100322877(CYP97C11)
SPEN107002514
SOT102591274
SSTN125865574
SDUL129875508
CANN107843608
LBB132607146
NTA107761917 107797056
NSY104210223
NTO104114442
NAU109237187
INI109181096
ITR116006108
SIND105174233
OEU111404128
EGT105952850
SSPL121798854
SMIL130997867
SHIS125199993 125217902
APAN127251569
HAN110882135
ECAD122581976
LSV111913167
CCAV112519301
DCR108220021
CSIN114262489 114262504
RVL131324690
AEW130752467
BVG104898583
SOE110788578
CQI110683094 110738483
ATRI130827141
MOF131167963
NNU104586192
MING122087098
TSS122655775
PSOM113313399
OSA4349290
DOSAOs10t0546600-01(Os10g0546600)
OBR102700091
OGL127753455
BDI100835344
ATS109740072
TDC119274766 119338491
TAES123051536 123127494 123181586
LPER127308289
LRD124706203
SBI8058776
ZMA103637620
SITA101758095
SVS117836012
PVIR120651787 120690677
PHAI112876006
PDA103719551
EGU105034264
MUS103970366
ZOF122003038 122007293
DCT110099518
PEQ110030448
AOF109825600
MSIN131224791
NCOL116253613
ATR18424541
SMOSELMODRAFT_186370(CYP97C15) SELMODRAFT_187123
PPP112283348
VCNVOLCADRAFT_83281
MNGMNEG_1212
CSLCOCSUDRAFT_63212
CVRCHLNCDRAFT_51247
APROF751_6907
OLUOSTLU_33533
OTAOT_ostta09g02540
BPGBathy10g01980
MISMICPUN_95887
MPPMICPUCDRAFT_32152
 » show all
Reference
1  [PMID:8837513]
  Authors
Pogson B, McDonald KA, Truong M, Britton G, DellaPenna D
  Title
Arabidopsis carotenoid mutants demonstrate that lutein is not essential for photosynthesis in higher plants.
  Journal
Plant Cell 8:1627-39 (1996)
DOI:10.1105/tpc.8.9.1627
Reference
2  [PMID:14709673]
  Authors
Tian L, Musetti V, Kim J, Magallanes-Lundback M, DellaPenna D.
  Title
The Arabidopsis LUT1 locus encodes a member of the cytochrome p450 family that is required for carotenoid epsilon-ring hydroxylation activity.
  Journal
Proc Natl Acad Sci U S A 101:402-7 (2004)
DOI:10.1073/pnas.2237237100
  Sequence
[ath:AT3G53130]
Reference
3  [PMID:21450689]
  Authors
Stigliani AL, Giorio G, D'Ambrosio C
  Title
Characterization of P450 carotenoid beta- and epsilon-hydroxylases of tomato and  transcriptional regulation of xanthophyll biosynthesis in root, leaf, petal and fruit.
  Journal
Plant Cell Physiol 52:851-65 (2011)
DOI:10.1093/pcp/pcr037
  Sequence
[sly:100322877]
Reference
4  [PMID:26030746]
  Authors
Chang S, Berman J, Sheng Y, Wang Y, Capell T, Shi L, Ni X, Sandmann G, Christou P, Zhu C
  Title
Cloning and Functional Characterization of the Maize (Zea mays L.) Carotenoid Epsilon Hydroxylase Gene.
  Journal
PLoS One 10:e0128758 (2015)
DOI:10.1371/journal.pone.0128758
Reference
5  [PMID:28858245]
  Authors
Reddy CS, Lee SH, Yoon JS, Kim JK, Lee SW, Hur M, Koo SC, Meilan J, Lee WM, Jang JK, Hur Y, Park SU, Kim AYB
  Title
Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora.
  Journal
Molecules 22:E1449 (2017)
DOI:10.3390/molecules22091449
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.158
IUBMB Enzyme Nomenclature: 1.14.14.158
ExPASy - ENZYME nomenclature database: 1.14.14.158
BRENDA, the Enzyme Database: 1.14.14.158

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